THE ORIGIN OF THE GENES THAT CONTROL PLACENTAL DEVELOPMENT: HOW LONG BEFORE THE APPEARANCE OF PLACENTAL ORGANISMS DID THEY ARISE?

Kadu Penuela Sanches Estevam; José Miguel Ortega

Título do Trabalho

Autores

Kadu Penuela Sanches Estevam
José Miguel Ortega

Modalidade

Poster

Área temática

Phylogeny and Evolution

Data de Publicação

02/10/2025

País da Publicação

Brazil | Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/xmeeting-2025/1066286-the-origin-of-the-genes-that-control-placental-development--how-long-before-the-appearance-of-placental-organism

ISBN

978-65-272-1722-0

Palavras-Chave

Keywords: gene evolution; ontology; phylogeny.

Resumo

We developed a web application named GO-Genesis (http://biodados.icb.ufmg.br/gogenesis) to trace the origin of proteins annotated to Gene Ontology Terms. Using this application, we discovered many terms with recent Least Common Ancestors (LCA) of targeted proteins, all implicated in Mammalian reproductive structures such as uterus, vagina, and sperm head. Here we analyze proteins associated with "Embryonic Placenta Development" (GO:0001892): the embryonically driven progression of placenta formation to maturity. The placenta is an organ of metabolic interchange between fetus and mother, partly of embryonic origin and partly of maternal origin. GO-Genesis identified Theria as the clade of origin for proteins from 56 species, with 25 proteins in Homo sapiens and 41 in Mus musculus. We analyzed each human protein using TaxOnTree, which performs BLAST searches in Uniprot Complete Proteomes Database, creates multiple alignments with MUSCLE, and builds trees with FastTree software. TaxOnTree adds taxonomic information to tree branches, enabling manual LCA analysis for each gene. The most recent gene, CSF2 (Granulocyte-macrophage colony-stimulating factor), appeared in Eutheria. This cytokine, which stimulates hematopoietic precursor cell growth and differentiation, likely contributed significantly to the GO process due to its recent origin. Heat shock factor protein 1 (HSP1) emerged in Tetrapoda. Several genes originated in aquatic vertebrates: HIF1A (Hypoxia-inducible factor 1-alpha) and PDFGB (Platelet-derived growth factor subunit B) in Sarcopterygii; ESRRB, TFEB, GATA2, and SP3 in Euteleostomi; and SPINT1, EGFR, IGF2, CEBPB, ARNT, PKD2, and EPAS1 in Gnathostomata. Six additional genes appeared in earlier organisms, from Metazoa to Bilateria. STRING-DB analysis mapped the protein interactions to embryonic placenta development, in utero embryonic development, reproductive structure development, and embryonic organ development. In Reactome, cellular response to hypoxia emerged as the predominant pathway, forming the second largest protein-protein interaction cluster in STRING-DB. These findings indicate that embryonic placenta development is primarily controlled by genes that arose in aquatic vertebrates, particularly from sharks to coelacanths, with two more recent additions: one in our common ancestor with frogs and another in placental organisms. A comprehensive pathway incorporating both original and interacting proteins is under development.

Título do Evento: 21º Congresso Brasileiro de Bioinformática: X Meeting 2025
Cidade do Evento: João Pessoa
Título dos Anais do Evento: Proceedings of the 21st Brazilian Congress on Bioinformatics (X-Meeting)
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

ESTEVAM, Kadu Penuela Sanches; ORTEGA, José Miguel. THE ORIGIN OF THE GENES THAT CONTROL PLACENTAL DEVELOPMENT: HOW LONG BEFORE THE APPEARANCE OF PLACENTAL ORGANISMS DID THEY ARISE?.. In: Proceedings of the 21st Brazilian Congress on Bioinformatics (X-Meeting). Anais...João Pessoa(PB) Centro Universitário - UNIESP, 2025. Disponível em: https//www.even3.com.br/anais/xmeeting-2025/1066286-THE-ORIGIN-OF-THE-GENES-THAT-CONTROL-PLACENTAL-DEVELOPMENT--HOW-LONG-BEFORE-THE-APPEARANCE-OF-PLACENTAL-ORGANISM. Acesso em: 20/10/2025