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This volume contains the official proceedings of the 21st Annual X-Meeting, the premier scientific conference hosted by the Brazilian Association of Bioinformatics and Computational Biology (AB3C). The event was held from June 03 to 06, 2025, in the city of João Pessoa, Paraiba, Brazil.

The 21st X-Meeting brought together a diverse community of leading researchers, academics, graduate and undergraduate students, and professionals from the biotechnology industry. The conference provided a fertile ground for the dissemination of scientific knowledge and the fostering of collaborative networks, reinforcing its status as the most significant event in the field within Brazil.

The comprehensive scientific program featured keynote lectures from internationally recognized experts, multiple sessions for oral presentations, extensive poster exhibitions, round-table discussions, and specialized workshops. The contributions presented and discussed encompassed a wide spectrum of bioinformatics and computational biology, including key areas such as:

Database and Software Development

DNA and Genomics

Education and outreach

Phylogeny and Evolution

Proteins and Proteomics

RNA and transcriptomics

Systems Biology and Modeling

The collection of work presented in these proceedings represents a valuable snapshot of the state-of-the-art research in the field at the time of the conference. It stands as a testament to the scientific advancements and collaborative spirit that characterize the mission of AB3C and the X-Meeting, contributing to the continued progress of science and technology in Brazil and globally.