Anais do IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS)

GENETIC DIVERSITY OF HEMOPLASMAS IN BATS FROM PARANÁ: PRELIMINARY RESULTS

Publicado em 10/03/2025 - ISBN: 978-65-272-1246-1

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Trabalho
Título do Trabalho
GENETIC DIVERSITY OF HEMOPLASMAS IN BATS FROM PARANÁ: PRELIMINARY RESULTS
Autores
  • Eduarda de Oliveira Silva Lima Machado
  • Isaac Leandro Lira Pinto
  • Thiago Dutra Dias
  • Laís Feliciano de Souza
  • Tatiana Pádua Tavares De Freitas
  • Letícia Pereira Da Silva
  • Victor Silva de Souza
  • Huarrisson Azevedo Santos
  • Bernardo Rodrigues Teixeira
  • Maristela Peckle Peixoto
Modalidade
Resumo
Área temática
Protozoários e Agentes transmitidos por vetores de importância em Saúde
Data de Publicação
10/03/2025
País da Publicação
Brasil
Idioma da Publicação
pt-BR
Página do Trabalho
https://www.even3.com.br/anais/ix-simposio-internacional-em-ciencias-veterinarias-sincvet-ix-international-symposium-in-veterinary-sciences-isvs-499466/1030930-genetic-diversity-of-hemoplasmas-in-bats-from-parana--preliminary-results
ISBN
978-65-272-1246-1
Palavras-Chave
Evolutionary distance, Phylogeny, Haplotypes, Hemotropic mycoplasmas
Resumo
New species and genotypes of hemotropic mycoplasmas have been described, but many remains to be investigated, especially in wild animals. The present study aimed to analyze the genetic diversity of hemotropic mycoplasmas in bats captured in Ponta Grossa, Paraná. Phylogenetic analysis was performed using a 970-bp 16S rDNA fragment database, which included a total of 30 Mycoplasma spp. sequences. Three sequences were obtained in this study, and 26 additional hemoplasma sequences were retrieved from GenBank. Mycoplasma pneumoniae was used as the outgroup. Alignment was carried out using the ClustalW method, and phylogenetic reconstruction was performed using maximum likelihood with 1,000 replicates. The best nucleotide substitution model was GTR+G+I. Genetic distances were calculated to evaluate intergroup, intragroup, and overall genetic distances using the maximum composite likelihood model. Analyses were performed using MEGA 11 software. For haplotype analysis, sequences of hemotropic mycoplasmas from the 'Haemofelis group' and the hemoplasmas obtained in this study were evaluated using DNAsp v. 6.12.03 software. The analysis determined the number of polymorphic sites (S), haplotypes (H), haplotype diversity (Hd), nucleotide diversity (p), and haplotype frequencies. A median-joining (MJ) haplotype network was constructed using PopART v. 1.7 to display intermediate haplotypes and mutations inferred from the analysis. Phylogenetic analysis placed the three sequenced samples from this study within the large 'Haemofelis group.' In this analysis, monophyletic clades with 5% or more evolutionary divergence between clades and high bootstrap support values were considered distinct genotypes and were designated A, B, C, D, E, F, G, and H. The overall mean distance was 11%, while the intragroup distances in the 'Haemofelis group' and the 'Suis group' were 7% and 8%, respectively. The intergroup distance was 21%. Two Mycoplasma sp. samples from Myotis riparius from this study clustered with hemoplasmas previously detected in Myotis lucifugus from the USA (KF713538) and Myotis chiloensis from Chile (MK295627), forming cluster C. One Mycoplasma sp. sample from Mimon benettii clustered with hemoplasmas detected in Desmodus rotundus from Peru (KY932722), forming cluster F. Nucleotide alignment polymorphism analysis of Mycoplasma 16S rDNA sequences identified 24 haplotypes with Hd = 0.997 ± 0.012, p = 0.06823 and S = 192. The haplotype network showed that each sequence corresponded to a unique and distinct haplotype, including the three sequences in this study, except for two that shared the same haplotype. These preliminary results indicate a high level of genetic diversity among the hemoplasmas detected in bats, as well as among other sequences of the large "Haemofelis group". These preliminary analyses provide valuable information on the Mycoplasmas of bats and will support future investigations of bacterial identification at the species level and epidemiology.
Título do Evento
IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS)
Título dos Anais do Evento
Anais do IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS)
Nome da Editora
Even3
Meio de Divulgação
Meio Digital

Como citar

MACHADO, Eduarda de Oliveira Silva Lima et al.. GENETIC DIVERSITY OF HEMOPLASMAS IN BATS FROM PARANÁ: PRELIMINARY RESULTS.. In: Anais do IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS). Anais...Seropédica(RJ) UFRRJ, 2025. Disponível em: https//www.even3.com.br/anais/ix-simposio-internacional-em-ciencias-veterinarias-sincvet-ix-international-symposium-in-veterinary-sciences-isvs-499466/1030930-GENETIC-DIVERSITY-OF-HEMOPLASMAS-IN-BATS-FROM-PARANA--PRELIMINARY-RESULTS. Acesso em: 11/03/2026

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