IN VITRO INFECTION OF ADULT FLEAS OF CTENOCEPHALIDES FELIS FELIS BY HETERORHABDITIS INDICA (LPP30) AND HETERORHABDITIS BACTERIOPHORA (HP88)

Ana Caroline Ferreira De Souza; Danielle Pereira Da Silva; Américo de Castro Monteiro Sobrinho; Marcia Cristina de Azevedo Prata; Thaís Ribeiro Correia Azevedo; Melissa Carvalho Machado do Couto Chambarelli

Título do Trabalho

Autores

Ana Caroline Ferreira De Souza
Danielle Pereira Da Silva
Américo de Castro Monteiro Sobrinho
Marcia Cristina de Azevedo Prata
Thaís Ribeiro Correia Azevedo
Melissa Carvalho Machado do Couto Chambarelli

Modalidade

Resumo

Área temática

Ectoparasitos: sistemática, epidemiologia e controle

Data de Publicação

10/03/2025

País da Publicação

Brasil

Idioma da Publicação

pt-BR

Página do Trabalho

https://www.even3.com.br/anais/ix-simposio-internacional-em-ciencias-veterinarias-sincvet-ix-international-symposium-in-veterinary-sciences-isvs-499466/1029637-in-vitro-infection-of-adult-fleas-of-ctenocephalides-felis-felis-by-heterorhabditis-indica-(lpp30)-and-heterorha

ISBN

978-65-272-1246-1

Palavras-Chave

: Entomopathogenic nematodes, Ctenocephalides felis felis, biological control, adults.

Resumo

Fleas of the genus Ctenocephalides felis felis are considered the most prevalent ectoparasites infesting dogs and cats and hold great importance in veterinary medicine, as they not only cause irritation and itching but also serve as vectors for various pathogens that can lead to diseases in animals and humans. Their control is typically carried out through the use of chemical products applied in infested environments and by treating animals with topical or oral formulations. However, in recent years, the indiscriminate and improper use of these products has contributed to the emergence of resistant populations of these arthropods in some regions of the world. Additionally, these substances can cause environmental contamination and mammalian intoxication, as well as represent a high financial cost. In this context, there is an increasing demand for alternative methods that can be used alone or in combination with conventional chemical products. Entomopathogenic nematodes (EPNs), widely used in the biological control of agricultural pests, have been studied as a promising option for controlling arthropods of veterinary relevance. The present study aimed to evaluate the infection and susceptibility of adult Ctenocephalides felis felis fleas by Heterorhabditis indica (LPP30) and Heterorhabditis bacteriophora (HP88) under laboratory conditions. The study was conducted in Annex 1 of LCM/UFRRJ. A total of 200 C. felis felis fleas were used for each species of EPNs, separated into 75 males and 75 females, placed in 6 plastic jars containing filter paper, with 25 fleas per jar and a suspension of 1500 µL of nematodes at a concentration of 2085 EPNs/jar for Heterorhabditis bacteriophora (HP88) and 2670 EPNs/jar for Heterorhabditis indica (LPP30). A control group was used, separated into 2 jars each containing 25 males and 25 females of adult C. felis felis fleas on filter paper and 1500 µL of distilled water. After infection, the samples were maintained in a BOD incubator at 25±1°C, 70% RH. Daily evaluations were conducted to verify flea susceptibility, and after 16 to 17 days, the adults were dissected. The evaluation of susceptibility and infection was conducted by observing the presence of EPNs within the fleas. During dissection, the presence of EPNs, both juveniles and adults, was observed within the fleas. No EPNs were observed within the adults in the control group. In the groups containing EPNs, 72% and 68% susceptibility rates were obtained for males and females of Heterorhabditis indica (LPP30), respectively, and 70.66% and 72% for Heterorhabditis bacteriophora (HP88) for males and females. Based on these results, it was possible to experimentally assess that adult fleas, both males and females, are susceptible to infection by these species of nematodes, which may in the future be used as a biological control method for this arthropod.

Título do Evento: IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS)
Título dos Anais do Evento: Anais do IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS)
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

SOUZA, Ana Caroline Ferreira De et al.. IN VITRO INFECTION OF ADULT FLEAS OF CTENOCEPHALIDES FELIS FELIS BY HETERORHABDITIS INDICA (LPP30) AND HETERORHABDITIS BACTERIOPHORA (HP88).. In: Anais do IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS). Anais...Seropédica(RJ) UFRRJ, 2025. Disponível em: https//www.even3.com.br/anais/ix-simposio-internacional-em-ciencias-veterinarias-sincvet-ix-international-symposium-in-veterinary-sciences-isvs-499466/1029637-IN-VITRO-INFECTION-OF-ADULT-FLEAS-OF-CTENOCEPHALIDES-FELIS-FELIS-BY-HETERORHABDITIS-INDICA-(LPP30)-AND-HETERORHA. Acesso em: 03/03/2026