DIAGNOSIS OF PLASMODIUM SP. (HAEMOSPORIDA: HAEMOPROTEIDAE) IN A FREE-LIVING SPECIES OF RAMPHASTOS TOCO STATIUS MULLER, 1776 (PICIFORMES: RAMPHASTIDAE) FROM RIO DE JANEIRO, BRAZIL

Marcos Pereira da Silva Junior; Maria izabel Fernandes Gouveia Pereira; Gilliard do Nascimento Ferreira; Davi Guimarães de Almeida; Jônathan David Ribas Chagas; Bruna de Azevedo Baêta; Adlilton Pacheco De Oliveira; Matheus Dias Cordeiro; Jeferson Rocha Pires; Claudia Bezerra da Silva

Título do Trabalho

Autores

Marcos Pereira da Silva Junior
Maria izabel Fernandes Gouveia Pereira
Gilliard do Nascimento Ferreira
Davi Guimarães de Almeida
Jônathan David Ribas Chagas
Bruna de Azevedo Baêta
Adlilton Pacheco De Oliveira
Matheus Dias Cordeiro
Jeferson Rocha Pires
Claudia Bezerra da Silva

Modalidade

Resumo

Área temática

Protozoários e Agentes transmitidos por vetores de importância em Saúde

Data de Publicação

10/03/2025

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/ix-simposio-internacional-em-ciencias-veterinarias-sincvet-ix-international-symposium-in-veterinary-sciences-isvs-499466/1028515-diagnosis-of-plasmodium-sp-(haemosporida--haemoproteidae)-in-a-free-living-species-of-ramphastos-toco-statius-m

ISBN

978-65-272-1246-1

Palavras-Chave

molecular detection, protozoan, wild animals, toucan

Resumo

Studies involving hemoparasites are frequent in wild animals kept under human care and wild animals, where the occurrence of infection becomes directly dependent on a vector. The use of quarantine and preventive medicine protocols is necessary to maintain the in situ and ex situ conservation of specimens. The objective of this study was to investigate the presence of Plasmodium sp. in free-living wild birds received at the Wild Animal Recovery Clinic of the Estácio de Sá University (CRAS-UNESA), Vargem Pequena campus, Rio de Janeiro (CEUA-Nº. 022/2019). Whole blood samples were collected in ethylenediamine tetraacetic acid (EDTA) and blood smears were taken from 55 free-living wild birds that arrived at CRAS-UNESA between the years 2022 and 2023. The blood smears were stained using the 10% Giemsa method and observed under light microscopy at 1000x magnification, and the presence of intraerythrocytic gametocytes was observed. The EDTA-conditioned samples were subjected to DNA (deoxyribonucleic acid) extraction using a commercial Invitrogen® kit and nested-PCR to amplify the ~479 base of pairs fragment of the CytB gene (Hemosporida target DNA). The target DNA was amplified in four (n=4) analyzed samples, obtaining a frequency of 7.27% (n=4/55) of positivity for hemosporids, of which 1 (one) was Ramphastos toco (Toucan), 1 (one) Rupornis magnirostris (Roadside Hawk) and 2 (two) Columbina talpacoti (Ruddy Ground-Dove). The Ramphastos toco sample was selected for sequencing using the Sanger method, showing 99.9% similarity with the Plasmodium sp. sequence (KX171628) found in the Ramphastos toco specimen kept under human care in the state of São Paulo. The finding indicates the formation of an exclusive clade of the two findings, standing out from the other Plasmodium species. This highlights the importance of studies on the Piciformes order and its parasites, given their vast variety in commercial and free-living farming.

Título do Evento: IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS)
Título dos Anais do Evento: Anais do IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS)
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

JUNIOR, Marcos Pereira da Silva et al.. DIAGNOSIS OF PLASMODIUM SP. (HAEMOSPORIDA: HAEMOPROTEIDAE) IN A FREE-LIVING SPECIES OF RAMPHASTOS TOCO STATIUS MULLER, 1776 (PICIFORMES: RAMPHASTIDAE) FROM RIO DE JANEIRO, BRAZIL.. In: Anais do IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS). Anais...Seropédica(RJ) UFRRJ, 2025. Disponível em: https//www.even3.com.br/anais/ix-simposio-internacional-em-ciencias-veterinarias-sincvet-ix-international-symposium-in-veterinary-sciences-isvs-499466/1028515-DIAGNOSIS-OF-PLASMODIUM-SP-(HAEMOSPORIDA--HAEMOPROTEIDAE)-IN-A-FREE-LIVING-SPECIES-OF-RAMPHASTOS-TOCO-STATIUS-M. Acesso em: 02/08/2025