MOLECULAR CHARACTERIZATION OF TRYPANOSOMA SP. IN WILD BIRDS IN DIFFERENT ALTITUDE GRADIENTS IN ITATIAIA NATIONAL PARK, BRAZIL.

Cristiane De Medeiros Campanha; Beatriz Bacelar Barbosa; Fernanda Gonçalves De Souza; Carla Alves Rabello; Júlia dos Santos Fonseca; Léo Paulis; Talys Henrique Assumpção Jardim; Patrícia Gonzaga Paulino; Huarrisson Azevedo Santos

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Trabalho

DOI

10.29327/9786527212461.1026978

Título do Trabalho

MOLECULAR CHARACTERIZATION OF TRYPANOSOMA SP. IN WILD BIRDS IN DIFFERENT ALTITUDE GRADIENTS IN ITATIAIA NATIONAL PARK, BRAZIL.

Autores

Cristiane De Medeiros Campanha
Beatriz Bacelar Barbosa
Fernanda Gonçalves De Souza
Carla Alves Rabello
Júlia dos Santos Fonseca
Léo Paulis
Talys Henrique Assumpção Jardim
Patrícia Gonzaga Paulino
Huarrisson Azevedo Santos

Modalidade

Resumo

Área temática

Protozoários e Agentes transmitidos por vetores de importância em Saúde

Data de Publicação

10/03/2025

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/ix-simposio-internacional-em-ciencias-veterinarias-sincvet-ix-international-symposium-in-veterinary-sciences-isvs-499466/1026978-molecular-characterization-of-trypanosoma-sp-in-wild-birds-in-different-altitude-gradients-in-itatiaia-national

ISBN

978-65-272-1246-1

Palavras-Chave

Trypanosoma, Atlantic Forest, wild birds, Phylogenetic analysis

Resumo

The rainforest is one of the largest centers of biodiversity and species endemism in the world. Relief is one of the elements responsible for producing so much diversity. Environmental conditions in the mountains are very different from those in lowland areas because they have a greater temperature range, water restrictions and greater geographical isolation. As a result, species experience adaptations that reflect environmental variations and can disrupt biotic exchanges with neighboring plains. Brazil has the greatest biological diversity on the planet, with more than 1,919 species of birds recorded in various ecosystems, above all in the Atlantic Rainforest, highlighted as a focal point for avifaunal diversity. This area is home to around 891 species, 203 of which are endemic. Most of these birds belong to the order Passerines , many of which are threatened with extinction. Hematozoan parasites such as Trypanosoma sp. are transmitted by a series of dipteran vectors abundantly present in this environment. Most susceptible hosts suffer from selective pressure, resulting in deleterious effects on the health, reproductive success, behavior and structure of wild avifauna. This study was carried out in regions of the Atlantic Forest, located in the Itatiaia National Park (PNI). The aim of this study was to correlate the elevation gradient of Itatiaia National Park with the prevalence and diversity of Trypanosoma sp. in the blood of wild birds using blood smears and PCR screening to verify the predominance of this hemoparasite. Of the 230 slide smears observed under microscopy, the infectious evolutionary form of Trypanosoma was found in 17 and 42 samples were positive for PCR. Prevalence in the lower elevation gradient, below 1,000, was higher than in the higher elevation gradient, above 2,000, which suggests that temperature, environmental conditions and vector diversity are more abundant in lower elevation areas, favoring transmission of the hematozoan. Phylogenetic analyses revealed 17 new sequences very close to Trypanosoma Polygranularis found in Peliperdix lathami in Cameroon, Africa. A single sequence clustered close to the Trypanosoma everetti clade, a branch close to the Trypanosoma everetti-like species found in the host Dumetella carolinensis, USA. Our study demonstrated the diversity and prevalence of trypanosome species in different altitudinal gradients in the PNI. It also pointed out that in Itatiaia National Park, the genetic diversity between Trypanosoma lineages in wild birds seems to be quite limited. Of the 18 sequences obtained during this study, an impressive 17 are closely related to Trypanosoma polygranularis according to their phylogenetic analysis. In addition, the Trypanosoma sp. strains identified in this research exhibit a wide host range, as they were observed in no less than 12 different bird species.

Título do Evento: IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS)
Título dos Anais do Evento: Anais do IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS)
Nome da Editora: Even3
Meio de Divulgação: Meio Digital
DOI

Como citar

CAMPANHA, Cristiane De Medeiros et al.. MOLECULAR CHARACTERIZATION OF TRYPANOSOMA SP. IN WILD BIRDS IN DIFFERENT ALTITUDE GRADIENTS IN ITATIAIA NATIONAL PARK, BRAZIL... In: Anais do IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS). Anais...Seropédica(RJ) UFRRJ, 2025. Disponível em: https//www.even3.com.br/anais/ix-simposio-internacional-em-ciencias-veterinarias-sincvet-ix-international-symposium-in-veterinary-sciences-isvs-499466/1026978-MOLECULAR-CHARACTERIZATION-OF-TRYPANOSOMA-SP-IN-WILD-BIRDS-IN-DIFFERENT-ALTITUDE-GRADIENTS-IN-ITATIAIA-NATIONAL. Acesso em: 20/07/2025