DEVELOPMENT AND CHARACTERIZATION OF 3D PRINTED CHITOSAN-BIOACTIVE GLASS SCAFFOLDS AIMING AT PERIPHERAL NERVE REGENERATION

Larissa RIbeiro Lourenço; Roger Borges; Mônica Beatriz Mathor; Carlos Alberto-Silva; Juliana Marchi

Título do Trabalho

Autores

Larissa RIbeiro Lourenço
Roger Borges
Mônica Beatriz Mathor
Carlos Alberto-Silva
Juliana Marchi

Modalidade

Oral Pitch and Poster

Área temática

3) Aplicações (uso de impressão 3D de materiais cerâmicos na área médica, odontológica, energia, industrial...)

Data de Publicação

05/11/2024

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/ceramicam-2024/880847-development-and-characterization-of-3d-printed-chitosan-bioactive-glass-scaffolds-aiming-at-peripheral-nerve-rege

ISBN

978-65-272-1074-0

Palavras-Chave

bioactive glass, chitosan, 3D printing, scaffold, nerve regeneration

Resumo

After a peripheral nerve injury, tissue regeneration and functional recovery are challenges to be overcome. Several materials and processing techniques have been studied to develop nervous scaffolds to support and improve the regenerative process. In such context, the development of chitosan-bioactive glass printed scaffolds is proposed to be investigated focusing on this application. Chitosan is a natural polymer that offers a biomimetic environment for cell development, and bioactive glasses (BG) can provide cell signaling through the ionic release that can enhance nervous tissue regeneration. The 3D printing technique allows the construction of complex designs with control of structural and biological properties. In this work, chitosan-BG composite scaffolds (BG concentration up to 5 wt%) were 3D printed to be applied in peripheral nerve regeneration. The scaffold properties were studied through scanning electron microscopy (SEM), swelling and degradation assays, and mechanical tensile analysis. Additionally, an indirect cytotoxicity assay was carried out to investigate the PC12 cell viability of the materials. Bioactive glass incorporation in the scaffold increased the apparent rugosity, allowed water absorption control, and optimized the degradation time of the scaffolds. Moreover, at lower concentrations, the addition of BG improved the mechanical properties of the scaffolds, while defects in their structure are present at higher BG amounts, leading to fragile behavior. The biological assay using PC-12 lineage showed that all scaffolds had high cell viability and could be successfully applied to tissue engineering. The overall results suggest that the BG addition improved the properties of the scaffolds for peripheral nerve regeneration.

Título do Evento: CeramicAM 2024 - 1º Encontro Brasileiro de Manufatura Aditiva de Cerâmica
Cidade do Evento: São Carlos
Título dos Anais do Evento: Proceedings of the Brazilian Meeting on Ceramic Additive Manufacturing (CeramicAM Brasil)
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

LOURENÇO, Larissa RIbeiro et al.. DEVELOPMENT AND CHARACTERIZATION OF 3D PRINTED CHITOSAN-BIOACTIVE GLASS SCAFFOLDS AIMING AT PERIPHERAL NERVE REGENERATION.. In: Proceedings of the Brazilian Meeting on Ceramic Additive Manufacturing (CeramicAM Brasil). Anais...São Carlos(SP) University of São Paulo, 2024. Disponível em: https//www.even3.com.br/anais/ceramicam-2024/880847-DEVELOPMENT-AND-CHARACTERIZATION-OF-3D-PRINTED-CHITOSAN-BIOACTIVE-GLASS-SCAFFOLDS-AIMING-AT-PERIPHERAL-NERVE-REGE. Acesso em: 29/05/2025