Proceedings of the 8th Brazilian Congress of Biotechnology

ANABASINE INTOXICATION AND ITS RELATION TO PLANT DEVELOMENT STAGES

Published in 21/11/2025 - ISBN: 978-65-272-1865-4

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Paper Title
ANABASINE INTOXICATION AND ITS RELATION TO PLANT DEVELOMENT STAGES
Authors
  • Vanessa Cristina Stein
  • Thaís Teixeira Valério Caetano
  • Thiago Alves Magalhães
  • Carlos Alexandre Carollo
  • Arthur Ladeira Macedo
  • Joao Maximo De Siqueira
Modality
Abstract
Subject area
Applications of Biotechnology in Environmental and Agricultural Areas;
Publishing Date
21/11/2025
Country of Publishing
Brazil | Brasil
Language of Publishing
en-US
Paper Page
https://www.even3.com.br/anais/biotecnologia2025/1181248-anabasine-intoxication-and-its-relation-to-plant-develoment-stages
ISBN
978-65-272-1865-4
Keywords
Alkaloid production, Invasive species, Nicotiana glauca, Plant phenology
Summary
Nicotiana glauca, commonly known as wild tobacco or tobacco bush, belongs to the Solanaceae family. It is native to South America but has become naturalized in various regions including Australia, California, Africa, and the Mediterranean. N. glauca is listed in the Global Invasive Species Database (GISD) and the Invasive Species Compendium (CABI). It is known for producing pyridine alkaloids, including anabasine, which is highly toxic. Anabasine is predominantly found in the leaves and can cause severe health issues such as neuromuscular blockade, respiratory arrest, and cardiovascular problems when ingested. Mistaken identity with edible plants like spinach has resulted in food poisoning cases in Israel and Brazil. Anabasine, a minor alkaloid constituent of tobacco, may contribute to tobacco addiction by mimicking or enhancing the effects of nicotine. Therefore, it is essential to investigate the production pattern of anabasine and its relationship to the developmental stages of the plant. This study aimed to establish the relationship between the phenological plant age, cultivation place, and the increase in anabasine concentration, which can lead to human intoxication cases.In this study, N. glauca plants were collected from three different rural areas in Brazil, during a year, to examine leaves at various stages of development. Samples were also obtained from cultivated plants in Marilândia, Minas Gerais, Brazil, as well as from Divinópolis, Minas Gerais, Brazil, and Arraial do Cabo, Rio de Janeiro, Brazil. In vitro cultivated plants on MS medium were included in the study. The collected leaves were dried, powdered, and stored. Alkaloid extraction was performed using a methanol and water mixture, followed by liquid-liquid extraction with chloroform. The anabasine content was determined using HPLC-DAD analysis with nicotine as a standard. The results indicated that anabasine production increases with the plant's development, peaking in adult leaves during the reproduction phase and declining afterward. In vitro plants showed similar anabasine production to young leaves. The successful adaptation of N. glauca in new environments poses a global problem, and the correlation between anabasine production and the plant's developmental stages has been understudied. The presence of substances produced by the plant can pose a risk to other species, especially when mistaken for edible plants. The findings from this study shed light on the pattern of anabasine production and its association with plant development, contributing to a better understanding of the potential risks associated with N. glauca and the importance of accurate identification. Acknowledgments: This study was supported by the Coordination for the Improvement of Higher Education Personnel – Brazil (CAPES) – Funding Code 001; the Research Support Foundation of the State of Minas Gerais (FAPEMIG); the National Council for Scientific and Technological Development (CNPq); the Civil Police of Minas Gerais (PCMG) in Lavras; the Brazilian Health Regulatory Agency (ANVISA); and the National Institute of Translational Science and Technology in Medicine (INCT-TM).
Title of the Event
8th Brazilian Biotechnology Congress.
City of the Event
Natal
Title of the Proceedings of the event
Proceedings of the 8th Brazilian Congress of Biotechnology
Name of the Publisher
Even3
Means of Dissemination
Meio Digital

How to cite

STEIN, Vanessa Cristina et al.. ANABASINE INTOXICATION AND ITS RELATION TO PLANT DEVELOMENT STAGES.. In: Proceedings of the 8th Brazilian Congress of Biotechnology. Anais...Natal(RN) Hotel Praia Mar, 2025. Available in: https//www.even3.com.br/anais/biotecnologia2025/1181248-ANABASINE-INTOXICATION-AND-ITS-RELATION-TO-PLANT-DEVELOMENT-STAGES. Access in: 11/03/2026

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