ORCHESTRATION DAYTIME METABOLISM OF SUGARCANE AND ENERGY-CANE: A METABOLOMIC, TRANSCRIPTOMIC AND NON-STRUCTURAL CARBOHYDRATE ANALYSIS

Jovanderson Jackson Barbosa da Silva; Luis Guilherme Furlan de Abreu; Allan Jhonathan Ramos Ferrari; Lucas Miguel de Carvalho; Adriana Grandis; Marcos Buckeridge; Taícia Pacheco Fill; Gonçalo Amarante Guimarães Pereira; Marcelo Falsarella Carazzolle

Paper Title

Authors

Jovanderson Jackson Barbosa da Silva
Luis Guilherme Furlan de Abreu
Allan Jhonathan Ramos Ferrari
Lucas Miguel de Carvalho
Adriana Grandis
Marcos Buckeridge
Taícia Pacheco Fill
Gonçalo Amarante Guimarães Pereira
Marcelo Falsarella Carazzolle

Modality

Xpress presentation

Subject area

Omics

Publishing Date

26/04/2022

Country of Publishing

Brasil

Language of Publishing

Inglês

Paper Page

https://www.even3.com.br/anais/xmeetingxp2021/423555-orchestration-daytime-metabolism-of-sugarcane-and-energy-cane--a-metabolomic-transcriptomic-and-non-structural-c

ISBN

978-65-5941-645-5

Keywords

Energy-cane, Sugarcane, Daytime metabolism

Summary

Energy-cane is a commercial hybrid originated from the crossing of commercial sugarcane (high sugar content) and Saccharum spontaneum (high fiber content) plants. The result of this cross resulted in plants with high fiber content, low sucrose content and higher productivity (2,5 times more) compared to commercial sugarcane hybrids, which made energy-cane an ideal plant to produce ethanol from second generation and bioelectricity. In Brazil, some companies have developed commercial varieties of energy-cane, such as GranBio S/A. Although sugarcane is widely studied from a genomic point of view and through molecular biology, very little is known about energy-cane. Here, we analyze carbohydrates and use transcriptomics and metabolomics approaches to understand how the profile of carbohydrates, metabolites and transcripts from the leaf (source) and stalk (sink) tissues of energy-cane and sugarcane behave during diurnal cycle and how these tissues interact metabolically with each other to adapt to the environment and sustain their growth. For this, we carried out an experiment in which the leaf and stalk tissues of sugarcane and energy-cane were collected during a 24-hour diurnal cycle. Then, carbohydrates were extracted and analyzed by HPAEC-PAD. To explore metabolic differences, samples were subjected to LC and GC-MS approaches. The spectra obtained were quantified by MZMine2 and the compounds identified through the GNPS database (Global Natural Product Social Molecular Networking) and as complementary identification we used CANOPUS (SIRIUS v. 4.9.3). To analyze the transcriptome, the RNA samples were sequenced by Illumina/HiSeq 4000. The transcriptome was assembled by Trinity (2.12.0), gene expression was obtained through Kallisto (v. 0.46.2) and annotation of transcripts was performed with PANNZER (Protein ANNotation with Z-scoRE). As a result, we identified that sugars (sucrose, glucose, fructose) and some metabolites underwent a phase shift during the diurnal variation in leaf and stem tissues and many biological pathways were more active in energy-cane than in sugarcane, such as the phenylpropanoid pathway. Although energy-cane has less sucrose than sugarcane, a higher concentration of starch was observed in the internodes of the energy-cane, suggesting that energy-cane uses this polymer to supply its bioenergetic metabolism. Most of the metabolites identified during the diurnal cycle were classified as organoheterocyclic compounds, oxygenated organic compounds, organic acids and derivatives, benzeneides, lipids and related molecules and phenylpropanoids. We also show that the greatest metabolic differences between sugarcane and energy-cane occur during the night period. The transcriptome is under analysis, it is expected that the expression of genes related to biomass production are more active in energy-cane, as seen in the metabolome. As next steps, we will integrate transcriptomics and metabolomics data through co-expression network analysis. Thus, the knowledge generated about the metabolism of energy-cane compared to sugarcane will enable the development of more productive varieties to supply the bioenergy sector.

Title of the Event: X-Meeting XPerience 2021
Title of the Proceedings of the event: X-Meeting presentations
Name of the Publisher: Even3
Means of Dissemination: Meio Digital

How to cite

SILVA, Jovanderson Jackson Barbosa da et al.. ORCHESTRATION DAYTIME METABOLISM OF SUGARCANE AND ENERGY-CANE: A METABOLOMIC, TRANSCRIPTOMIC AND NON-STRUCTURAL CARBOHYDRATE ANALYSIS.. In: X-Meeting presentations. Anais...São Paulo(SP) AB3C, 2021. Available in: https//www.even3.com.br/anais/xmeetingxp2021/423555-ORCHESTRATION-DAYTIME-METABOLISM-OF-SUGARCANE-AND-ENERGY-CANE--A-METABOLOMIC-TRANSCRIPTOMIC-AND-NON-STRUCTURAL-C. Access in: 04/06/2025