X-Meeting presentations

NEW INSIGHTS THROUGH RE-SEQUENCING AND CORRECTION OF ASSEMBLY IN CORYNEBACTERIUM PSEUDOTUBERCULOSIS GENOMES

Published in 26/04/2022 - ISBN: 978-65-5941-645-5

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Paper Title
NEW INSIGHTS THROUGH RE-SEQUENCING AND CORRECTION OF ASSEMBLY IN CORYNEBACTERIUM PSEUDOTUBERCULOSIS GENOMES
Authors
  • Thiago de Jesus Sousa
  • Anne Cybelle Pinto Gomide
  • Diego Lucas Neres Rodrigues
  • Rodrigo Profeta
  • Arun Kumar Jaiswal
  • Rodrigo Dias de Oliveira Carvalho
  • Flávia Figueira Aburjaile
  • Nubia Seyffert
  • Thiago Luiz de Paula Castro
  • MATEUS MATIUZZI DA COSTA
  • Renata de Faria Silva Souza
  • Bertram Brenig
  • Siomar de Castro Soares
  • Vasco Ariston de Carvalho Azevedo
Modality
Xpress presentation
Subject area
Omics
Publishing Date
26/04/2022
Country of Publishing
Brasil
Language of Publishing
Inglês
Paper Page
https://www.even3.com.br/anais/xmeetingxp2021/422924-new-insights-through-re-sequencing-and-correction-of-assembly-in-corynebacterium--pseudotuberculosis-genomes
ISBN
978-65-5941-645-5
Keywords
Corynebacterium pseudotuberculosis, Illumina Hiseq, Genome assembly, SNPs, Pan-genomic.
Summary
Due to the continuous increase of the accessibility of genomes sequenced on NGS platforms, it has been possible to cover different platforms and perform quality assembly, achieving better accuracy in the genomic data. However, a combination of high-performance assembly strategies, such as the optical map, ordering by reference, and validation by the paired library, is still necessary for robust results. From high-quality assembled genomes, it is possible to perform comparative genomics to investigate the organism's biology based on the gene sequences and genome plasticity analyses. In this way, this work contemplates the genomic analysis of 50 strains of Corynebacterium pseudotuberculosis biovar ovis, a bacterium distributed worldwide, which causes different diseases in different hosts. The genomes were sequenced, assembled, and validated for structural analysis. This sequencing technology was selected (Illumina Hiseq 2500) due to the high quality of reads with an average quality score Phred score of more than 35 and coverage of ~900-fold. The ab initio assembly method was efficient, making it possible to generate assemblies with an average of 8 contigs per genome. Then, after gap closure, all 50 genomes were deposited as complete genomes at GenBank. This strategy made it possible to update pan genomic studies, where new genes were identified and the absence of others that had not been previously described. Also, it was possible to identify SNPs and non-synonymous mutations. Thus, with an entire set of 50 strains of the biovar ovis and using the Cp1002 strain (GCA_000144935.3) as a reference, a total of 3410 SNPs were detected. After comparisons and gene predictions, 2836 mutations were detected in protein-coding regions. The new genome versions, including strains from different countries and hosts, with high quality, may contribute to discoveries on new targets for vaccines and drugs against this bacterium, which causes significant damage to agribusiness.
Title of the Event
X-Meeting XPerience 2021
Title of the Proceedings of the event
X-Meeting presentations
Name of the Publisher
Even3
Means of Dissemination
Meio Digital

How to cite

SOUSA, Thiago de Jesus et al.. NEW INSIGHTS THROUGH RE-SEQUENCING AND CORRECTION OF ASSEMBLY IN CORYNEBACTERIUM PSEUDOTUBERCULOSIS GENOMES.. In: X-Meeting presentations. Anais...São Paulo(SP) AB3C, 2021. Available in: https//www.even3.com.br/anais/xmeetingxp2021/422924-NEW-INSIGHTS-THROUGH-RE-SEQUENCING-AND-CORRECTION-OF-ASSEMBLY-IN-CORYNEBACTERIUM--PSEUDOTUBERCULOSIS-GENOMES. Access in: 18/07/2025

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