X-Meeting presentations

POTENTIAL OF THE OLFACTORY RECEPTOR OR51F2 GENE AS BIOMARKER TO METASTATIC CERVICAL CANCER

Published in 26/04/2022 - ISBN: 978-65-5941-645-5

Link All Papers
LinkSubmission
Paper Title
POTENTIAL OF THE OLFACTORY RECEPTOR OR51F2 GENE AS BIOMARKER TO METASTATIC CERVICAL CANCER
Authors
  • Natália Gregório Custódio
  • Angelo Neto
  • Tamires Caixeta Alves
  • Álvaro Percínio Costa
  • Laurence Rodrigues do Amaral
  • Paulo Guilherme O. Salles
  • Matheus Gomes
  • Wander Jesus Jeremias
  • Leticia da Conceição Braga
  • Fábio Ribeiro Queiroz
Modality
Xpress presentation
Subject area
Omics
Publishing Date
26/04/2022
Country of Publishing
Brasil
Language of Publishing
Inglês
Paper Page
https://www.even3.com.br/anais/xmeetingxp2021/420081-potential-of-the-olfactory-receptor-or51f2-gene-as-biomarker-to-metastatic-cervical-cancer
ISBN
978-65-5941-645-5
Keywords
Cervical cancer, biomarkers, metastasis, prognosis
Summary
Cervical cancer (CC) is one of the most common malignancies that affect women. According to the World Health Organization, it is the second leading cause of cancer mortality in patients aged 20 to 39 years and has a significant impact on women's health. Even with the prevention, screening, and diagnosis protocols applied to the pathology, the prognosis of patients with cervical cancer remains ineffective, especially in metastatic ones. The present study aimed to explore the olfactory receptor family 51 (OR51) as a differentially expressed gene (DEG) between Nonmetastatic (NM) and Metastatic (M) patients with cervical cancer through high throughput sequencing. Thus, 7 M and 8 NM patients were selected. The cell suspensions were produced from the biopsies and used for fluorescence activated cell sorting (FACS). Stem and nonstem tumoral cells were separately collected and sequenced using the Illumina platform. Only nonstem cells were analyzed in the present study. The expression analysis was performed with DESeq2, considering differentially expressed genes (DEGs) those with logfoldchange > 1 or < -1 and padj < 0,05. The statistical Wald test and adjustment p-value with method Benjamini Hochberg was also performed with DESeq2. OR51F2 was found differentially expressed when comparing NM and M patients, with evident overexpression in group M, similarly to the VEGFA gene, the hallmark of cancer. Paradoxically, genes associated with epithelial mesenchymal transition (EMT) and metastases were upregulated in NM patients, suggesting that these patients, who present invasive features, appear to be more susceptible to metastatic dissemination. Data indicate, therefore, that the OR51F2 gene is expected to be a potential biomarker of metastatic status in CC, which corroborates other studies involving olfactory receptors for the disease in different sites, such as breast cancer. These results open the way towards understanding the role of this gene as a biomarker for CC. Additional studies are already in progress to elucidate this.
Title of the Event
X-Meeting XPerience 2021
Title of the Proceedings of the event
X-Meeting presentations
Name of the Publisher
Even3
Means of Dissemination
Meio Digital

How to cite

CUSTÓDIO, Natália Gregório et al.. POTENTIAL OF THE OLFACTORY RECEPTOR OR51F2 GENE AS BIOMARKER TO METASTATIC CERVICAL CANCER.. In: X-Meeting presentations. Anais...São Paulo(SP) AB3C, 2021. Available in: https//www.even3.com.br/anais/xmeetingxp2021/420081-POTENTIAL-OF-THE-OLFACTORY-RECEPTOR-OR51F2-GENE-AS-BIOMARKER-TO-METASTATIC-CERVICAL-CANCER. Access in: 31/05/2025

Paper

Even3 Publicacoes