A PHYLOGENETIC STUDY OF THE  TRYPANOSOMATIDS TTAGGG REPEAT-BIND  FACTOR PROTEIN (TRF)

Arthur de Oliveira Passos; Maria Isabel Nogueira Cano

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Paper Title

A PHYLOGENETIC STUDY OF THE TRYPANOSOMATIDS TTAGGG REPEAT-BIND FACTOR PROTEIN (TRF)

Authors

Arthur de Oliveira Passos
Maria Isabel Nogueira Cano

Modality

Poster

Subject area

Phylogeny and Evolution

Publishing Date

08/11/2023

Country of Publishing

Brazil | Brasil

Language of Publishing

Inglês

Paper Page

https://www.even3.com.br/anais/xmeeting2023/643314-a-phylogenetic-study-of-the--trypanosomatids-ttaggg-repeat-bind--factor-protein-(trf)

ISBN

978-65-272-0061-1

Keywords

TRF, Shelterin, Telomere, trypanosomatids

Summary

At the end of each linear chromosome are nucleoprotein structures called telomeres, whose function is to protect and maintain the integrity of the genetic material. Telomeres are formed by tandem repeats of 5'-TTAGGG-3' associated with proteins such as components of the shelterin and CST complexes. Telomere structure is conserved in most eukaryotes, including protozoa of the genera Leishmania and Trypanosoma, obligate parasites of great medical relevance. The shelterin complex plays an important role in telomere protection and maintenance through its involvement with the regulation of telomere metabolism. Among these components are the TRF1 and TRF2 (TTAGG repeat-bind factor), which regulate telomerase activity in vertebrates, the enzyme responsible for telomere elongation. In addition, the TRF1 and TRF2 proteins are involved in T-loop formation, structures that protect the telomere from nuclease attack and prevent it from being recognized as DNA double-stranded break. Unlike the two isoforms found in humans, in trypanosomatids, there is a unique ortholog of the TRF proteins. The trypanosomatids TRF has been characterized in Trypanosoma brucei and partially in Leishmania amazonensis. In T. brucei, its interaction with other telomeric proteins demonstrated that it plays an important role in telomere metabolism, being considered a good target for drug design. The present work aims to characterize in silico the trypanosomatids TRF, seeking to identify conserved and specific domains and motifs in the putative sequences available in the protein databases. Based on the alignment with the T-coffee software, our preliminary data suggest the presence of a conserved myb domain involved in protein-telomeric DNA interaction and the homodimerization TRFH domain. The trypanosomatids TRFs also share a greater identity with the human TRF2, although they lack the N-terminal basic domain involved with chromatin condensation. Phylogenetic analysis showed the protein in the most diverse genera of the trypanosomatids group, indicating it is preserved along the taxon. Since the vertebrate TRFs seemed to be originated from a duplication of an ancestral gene, we are analyzing other trypanosomatid telomeric proteins, such as TBP1, that also bears a canonical myb binding domain, to check their potentiality to be another TRF. This work intends to elucidate the evolutionary history of the TRF protein in trypanosomatids.

Title of the Event: X-Meeting / BSB 2023
City of the Event: Curitiba
Title of the Proceedings of the event: X-Meeting presentations
Name of the Publisher: Even3
Means of Dissemination: Meio Digital

How to cite

PASSOS, Arthur de Oliveira; CANO, Maria Isabel Nogueira. A PHYLOGENETIC STUDY OF THE TRYPANOSOMATIDS TTAGGG REPEAT-BIND FACTOR PROTEIN (TRF).. In: X-Meeting presentations. Anais...Curitiba(PR) Campus da indústria, 2023. Available in: https//www.even3.com.br/anais/xmeeting2023/643314-A-PHYLOGENETIC-STUDY-OF-THE--TRYPANOSOMATIDS-TTAGGG-REPEAT-BIND--FACTOR-PROTEIN-(TRF). Access in: 29/08/2025