X-Meeting presentations

DIFFERENTIAL GENE EXPRESSION IN SKELETAL MUSCLE OF PIGS WITH EXTREME VALUES OF OLEIC ACID CONTENT

Published in 08/11/2023 - ISBN: 978-65-272-0061-1

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Paper Title
DIFFERENTIAL GENE EXPRESSION IN SKELETAL MUSCLE OF PIGS WITH EXTREME VALUES OF OLEIC ACID CONTENT
Authors
  • Izally Carvalho Gervásio
  • Simara Larissa Fanalli
  • Lucas Echevarria Nascimento
  • Mariah Castro Durval
  • Heidge Fukumasu
  • Júlio Cesar de Carvalho Balieiro
  • Luciana Correia De Almeida Regitano
  • Luiz F. Brito
  • Aline Silva Mello Cesar
Modality
Poster
Subject area
RNA and transcriptomics
Publishing Date
08/11/2023
Country of Publishing
Brazil | Brasil
Language of Publishing
Inglês
Paper Page
https://www.even3.com.br/anais/xmeeting2023/643296-differential-gene-expression-in-skeletal-muscle-of-pigs-with-extreme-values-of-oleic-acid-content
ISBN
978-65-272-0061-1
Keywords
RNA-Seq, differential expression, pork, DESeq2
Summary
The meat's fatty acid composition is directly linked with pork quality, eating experience, and nutritional value for human health. Monounsaturated oleic acid (OA, C18:1) is a fatty acid associated with a lower risk of human metabolic diseases, including inflammation and obesity. The proportion of OA in intramuscular fat (IMF) is highly influenced by the animals’ diet and genetic background, and it can modulate the gene expression level in different tissues. Considering the importance of OA for human health and its relationship with metabolic processes, the main objectives of this study were to investigate the differences in the gene expression level in the skeletal muscle of pigs the Large White line that deposited extreme levels of OA in IMF when fed a basal diet supplemented with canola oil (CO), soybean oil (SOY), or fish oil (FO) and identify metabolic pathways and gene networks involved in the metabolic processes identified. This study was approved by the Animal Care and Use Committee of Luiz de Queiroz College of Agriculture (ESALQ/USP). Among 54 Large White immunocastrated male pigs, 9 and 11 presented low and high OA content in IMF, respectively, based on the adjusted phenotypic values by a linear model in the R software. The Tukey test was used to identify significant differences between treatments at a significance level of 5%. RNA-Seq data from the selected animals were used to identify differentially expressed genes (DEG) in the skeletal muscle of pigs. The quality of the raw RNA-Seq reads was evaluated using the FastQC v.0.11.8 software. The reads were filtered using Trim Galore v.0.6.5 software and aligned to the Sus Scrofa 11.1 reference genome available on the Ensembl database. The mRNA abundance for all annotated genes was calculated using the STAR v.2.7.6a package and gene expression levels were normalized using the R package DESeq2. The results obtained through the statistical mean test performed between the high and low OA groups showed significant differences (p < 0.05). A total of 142 DEG (log2-FC difference = 1 or = - 1; FDR-corrected p-value < 0.1) were identified between the group of animals with low and high OA deposition in IMF. Of these, 69 DEG were down-regulated and 73 were up-regulated. Among the up-regulated genes, SLC9A3 (log2FC +2.55), NCEH1 (log2FC +1.88), and EPHX1 (og2FC +1.78) are key candidate genes related to the bile secretion pathway. On the other hand, the adipocyte lipolysis regulation pathway was enriched with down-regulated genes, including GNAI1 (log2FC -1.63), PLIN1 (log2FC -3.90), and IRS3 (log2FC -3.99). These findings provide important insights into the mechanisms involved in OA deposition in pork meat and may contribute to the development of nutritional strategies to improve meat quality.
Title of the Event
X-Meeting / BSB 2023
City of the Event
Curitiba
Title of the Proceedings of the event
X-Meeting presentations
Name of the Publisher
Even3
Means of Dissemination
Meio Digital

How to cite

GERVÁSIO, Izally Carvalho et al.. DIFFERENTIAL GENE EXPRESSION IN SKELETAL MUSCLE OF PIGS WITH EXTREME VALUES OF OLEIC ACID CONTENT.. In: X-Meeting presentations. Anais...Curitiba(PR) Campus da indústria, 2023. Available in: https//www.even3.com.br/anais/xmeeting2023/643296-DIFFERENTIAL-GENE-EXPRESSION-IN-SKELETAL-MUSCLE-OF-PIGS-WITH-EXTREME-VALUES-OF-OLEIC-ACID-CONTENT. Access in: 30/08/2025

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