EVALUATING THE MICROBIOME-HOST INTERACTION OF A CALCAREOUS ALGA (CCA)

Carolina Salvador Duque Estrada; Diogo Antonio Tschoeke; Fabiano Thompson; Giselle Cavalcanti

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Submission

Paper Title

EVALUATING THE MICROBIOME-HOST INTERACTION OF A CALCAREOUS ALGA (CCA)

Authors

Carolina Salvador Duque Estrada
Diogo Antonio Tschoeke
Fabiano Thompson
Giselle Cavalcanti

Modality

Poster

Subject area

RNA and transcriptomics

Publishing Date

08/11/2023

Country of Publishing

Brazil | Brasil

Language of Publishing

Inglês

Paper Page

https://www.even3.com.br/anais/xmeeting2023/642506-evaluating-the-microbiome-host-interaction-of-a-calcareous-alga-(cca)

ISBN

978-65-272-0061-1

Keywords

rhodolite; metatranscriptome; microbiome

Summary

The rhodoliths are associated with a diverse microbial consortium, which play a fundamental role in their functioning. Changes in ocean carbonate chemistry driven by increased anthropogenic carbon dioxide (CO2) emissions promote ocean acidification. Thus, evaluating the microbial responses to environmental stresses in calcareous organisms such as rhodoliths becomes essential to assess the health of the host. The objective of the study is to describe, from the metatranscriptome, the microbiome associated with the holobiont Lithothamnion crispatum, from Santa Bárbara Island, in the Abrolhos Archipelago, Bahia (Brazil). Through in silico analyses, a total of twelve sequences were evaluated, nine referring to viable rhodoliths and three to the calcareous skeleton (non-viable), in two conditions of pH 8 (natural environment) and 7 (acidic environment). Raw data were pre-processed using Pinseq software (quality control), RiboPicker (ribosomal RNA removal), while the taxonomic and functional profile was evaluated using the MG-RAST program, while gene expression was assigned to the Gene Ontology server. The taxonomic characterization showed that the phyla Cyanobacteria, Proteobacteria, Bacteroidete, Firmicute, Thaumarchaeota and Virus are the most abundant in viable rhodoliths, while in the calcareous skeleton the presence of the same phyla was observed, with the exception of Cyanobacteria. In the results, it is possible to observe a microbial dynamics preserved in healthy rhodoliths and another composition preserved in the calcareous skeleton, both of which did not modulate their biofilms in relation to pH. The functional profile showed that the most active subsystems were photosynthesis, protein metabolism, respiration, cluster-based subsystem, carbohydrates, phage, prophage, transposable elements, plasmid and amino acids. In the nineteen major gene products observed, they are shown to be related to functions such as photosynthesis, respiration, protein metabolism, phage and prophage processing. These results infer the importance of the host-microbe interaction as an observation point for the conservation of rhodolith beds located in Brazil.

Title of the Event: X-Meeting / BSB 2023
City of the Event: Curitiba
Title of the Proceedings of the event: X-Meeting presentations
Name of the Publisher: Even3
Means of Dissemination: Meio Digital

How to cite

ESTRADA, Carolina Salvador Duque et al.. EVALUATING THE MICROBIOME-HOST INTERACTION OF A CALCAREOUS ALGA (CCA).. In: X-Meeting presentations. Anais...Curitiba(PR) Campus da indústria, 2023. Available in: https//www.even3.com.br/anais/xmeeting2023/642506-EVALUATING-THE-MICROBIOME-HOST-INTERACTION-OF-A-CALCAREOUS-ALGA-(CCA). Access in: 22/05/2025