DE NOVO TRANSCRIPTOME ASSEMBLY AND FUNCTIONAL ANNOTATION OF THE ROSETTE AGENT: SPHAEROTHECUM DESTRUENS (OPISTHOKONTA; ICHTHYOSPOREA)

David Aciole Barbosa; Paulo Sallarola Takao; Rafaela de Campos Oliveira; Daniela Leite Jabes; Luiz Nunes; Fabiano Menegidio

Paper Title

Authors

David Aciole Barbosa
Paulo Sallarola Takao
Rafaela de Campos Oliveira
Daniela Leite Jabes
Luiz Nunes
Fabiano Menegidio

Modality

Poster

Subject area

RNA and transcriptomics

Publishing Date

08/11/2023

Country of Publishing

Brazil | Brasil

Language of Publishing

Inglês

Paper Page

https://www.even3.com.br/anais/xmeeting2023/637206-de-novo-transcriptome-assembly-and-functional-annotation-of-the-rosette-agent--sphaerothecum-destruens-(opisthoko

ISBN

978-65-272-0061-1

Keywords

Sphaerothecum destruens, RNA-seq, de novo transcriptome, rosette agent, disease

Summary

Sphaerothecum destruens, also known as the rosette agent, is one of the most important intracellular parasites of fish. The genus Sphaerotecum has only two species, S. destruens and S. caipira, and is currently classified in clade Opisthokonta, class Ichthyosporea (Mesomycetozoea). Such taxonomy place it close to metazoa and fungi, but the term ‘protist’ or ‘fungus-like’ is used to refer to Ichthyosporeans, which includes Dermocystida order, comprising several other parasites of amphibians and fishes. S. destruens, for instance, causes diseases in salmoniformes (salmons) and cypriniformes (carps), a problem that is expected to cause serious economic damage. Nonetheless, genomic information for S. destruens is extremely scarce, while no genome or transcriptome assembly exist for this parasite. Therefore, in the present report the first de novo transcriptome assembly and functional annotation of Sphaerotecum destruens is brought to light. The de novo transcriptome of S. destruens was assembled with Trinity and characterized comprising of 12,000 genes and 19,720 transcripts, with 42.35 GC%. Contigs assembled have median and average length of 756 and 1,086, respectively, in a total of 21,415,945 assembled bases. Transcriptome completeness assessed by Benchmarking Universal Single-Copy Orthologs (BUSCO), identified 76.8% of the universal complete genes in Eukaryote database. With the aid of Eukaryotic Non-Model Transcriptome Annotation Pipeline (EnTAP), 13.624 (69%) elements received functional annotation from NCBI's nr and RefSeq databases and from EggNOG database: 13.066 (66%) annotated to protein domains; 12.493 (63%) were assigned at least one Gene Ontology; 11.942 (60%) to Biological Process; 10.316 (52%) to Cellular Component; and (58%) to Molecular Function.

Title of the Event: X-Meeting / BSB 2023
City of the Event: Curitiba
Title of the Proceedings of the event: X-Meeting presentations
Name of the Publisher: Even3
Means of Dissemination: Meio Digital

How to cite

BARBOSA, David Aciole et al.. DE NOVO TRANSCRIPTOME ASSEMBLY AND FUNCTIONAL ANNOTATION OF THE ROSETTE AGENT: SPHAEROTHECUM DESTRUENS (OPISTHOKONTA; ICHTHYOSPOREA).. In: X-Meeting presentations. Anais...Curitiba(PR) Campus da indústria, 2023. Available in: https//www.even3.com.br/anais/xmeeting2023/637206-DE-NOVO-TRANSCRIPTOME-ASSEMBLY-AND-FUNCTIONAL-ANNOTATION-OF-THE-ROSETTE-AGENT--SPHAEROTHECUM-DESTRUENS-(OPISTHOKO. Access in: 25/05/2025