X-Meeting presentations

RETRO-MIRS: NOVEL AND FUNCTIONAL MIRNAS ORIGINATED FROM MRNA RETROTRANSPOSITION

Published in 08/11/2023 - ISBN: 978-65-272-0061-1

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Paper Title
RETRO-MIRS: NOVEL AND FUNCTIONAL MIRNAS ORIGINATED FROM MRNA RETROTRANSPOSITION
Authors
  • Rafael Luiz Vieira Mercuri
  • Helena Beatriz da Conceição
  • Gabriela Guardia
  • Gabriel Nassar Reich Goldstein
  • Maria Dulcetti Vibranovski
  • Ludwig Christian Hinske
  • Pedro A F Galante
Modality
Poster
Subject area
Phylogeny and Evolution
Publishing Date
08/11/2023
Country of Publishing
Brazil | Brasil
Language of Publishing
Inglês
Paper Page
https://www.even3.com.br/anais/xmeeting2023/634657-retro-mirs--novel-and-functional-mirnas-originated-from-mrna-retrotransposition
ISBN
978-65-272-0061-1
Keywords
miRNAs and Retrocopies
Summary
Reverse transcribed gene copies, aka retrocopies, have been recognized as a major source of evolutionary novelties. MicroRNAs (miRNAs) are small, highly conserved RNAs molecules among species that serve as key post-transcriptional regulators of gene expression. Although the birth and subsequent evolution of miRNAs have been addressed, but not fully. In this study, we carried out a comprehensive investigation of miRNAs origination through retroduplicated mRNA sequences (retrocopies). We identified 17 retroduplicated miRNAs (retro-miRs) that emerged from mRNAs retrocopies. Four of these retro-miRs had de novo origination within retrocopied sequences, while 13 retro-miRNAs were located within exon regions and were duplicated along with their host mRNAs. We found that retro-miRs are primates specific, including 5 retro-miRs conserved among all primates and two human-specific retro-miRs. All of the retro-miRs were expressed and had predicted and experimentally validated target genes, with the exception of miR-10527. Notably, the target genes of retro-miRs are involved in key biological processes, such as metabolic processes, cell signaling and regulation of neurotransmitters in the central nervous system. Additionally, we found that these retro-miRs have a potential oncogenic role in cancer, targeting key cancer genes and being overexpressed in several cancer types, including Liver Hepatocellular Carcinoma and Stomach Adenocarcinoma. Our findings demonstrate that mRNAs retrotransposition is a key mechanism for the generation of novel miRNAs (retro-miRs) in primates. These retro-miRs are expressed, conserved, have target genes with important cellular functions, and play roles in cancer. These findings provide a significant contribution to our understanding of miRNAs' evolution and the impact of retrocopies on gene regulation and disease.
Title of the Event
X-Meeting / BSB 2023
City of the Event
Curitiba
Title of the Proceedings of the event
X-Meeting presentations
Name of the Publisher
Even3
Means of Dissemination
Meio Digital

How to cite

MERCURI, Rafael Luiz Vieira et al.. RETRO-MIRS: NOVEL AND FUNCTIONAL MIRNAS ORIGINATED FROM MRNA RETROTRANSPOSITION.. In: X-Meeting presentations. Anais...Curitiba(PR) Campus da indústria, 2023. Available in: https//www.even3.com.br/anais/xmeeting2023/634657-RETRO-MIRS--NOVEL-AND-FUNCTIONAL-MIRNAS-ORIGINATED-FROM-MRNA-RETROTRANSPOSITION. Access in: 27/05/2025

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