STUDY OF METABOLIC CHANGES INDUCED BY SARS-COV-2 SPIKE PROTEIN IN ZEBRAFISH (DANIO RERIO) WITH LOW-LEVEL LASER AS ANTI-INFLAMMATORY THERAPY.

Leonardo Santos Alexandre; Vinícius Guimarães Ferreira; Ives Charlie da Silva; Emanuel Carrilho

Paper Title

Authors

Leonardo Santos Alexandre
Vinícius Guimarães Ferreira
Ives Charlie da Silva
Emanuel Carrilho

Modality

Poster

Subject area

Systems Biology and Modeling

Publishing Date

08/11/2023

Country of Publishing

Brazil | Brasil

Language of Publishing

Inglês

Paper Page

https://www.even3.com.br/anais/xmeeting2023/628142-study-of-metabolic-changes-induced-by-sars-cov-2-spike-protein-in-zebrafish-(danio-rerio)-with-low-level-laser-as

ISBN

978-65-272-0061-1

Keywords

COVID-19, Zebrafish, Low Level Laser, Metabolomics, Bioinformatics, Mass Spectrometry, Liquid Chromatography.

Summary

The SARS-CoV-2 virus responsible for the COVID-19 pandemic acts in the organism hijacking the cellular biochemical machinery and using it for viral replication. This invasion promotes many metabolic changes within the cells. Also, most severe cases suffer from cytokine storm, an exacerbated immune system reaction that can cause death. Low-Level Laser Therapy (LLLT) has a high potential to treat inflammatory disorders, like cytokine storms, directly affecting the cell ATP production, dissociating NO, and promoting reactive oxygen species formation, modulating the expression of different second messengers. To study the metabolism, the use of animal models proves to be helpful, presenting a controlled environment to make scientific observations. The zebrafish (Danio rerio) animal model is very interesting for inflammatory disease studies. It has an immune system response very close to that of humans. With SARS-CoV-2 spike protein, the zebrafish organism can undergo an inflammatory state with cytokine release. The metabolism and its changes are studied by metabolomics, which focuses on understanding biological differences at a subcellular level and can help search for biomarkers, biological mechanisms, or evidence that can support the treatment or comprehension of a disease. In this work, the animals were separated into three groups: The control group was treated with LLLT, one group was exposed to SARS-CoV-2 spike protein, and the last was treated with LLLT after exposure to spike protein. The samples were collected in two-time frames, 6 h, and 24 h. Metabolites were extracted by Bligh and Dyer procedure, and UHPLC-MS further analyzed the lipidic fraction. The data were treated with MZmine software and submitted to the MetaboAnalyst platform for statistical analysis. There was realized ANOVA, PCA and PLS-DA analysis, and the 15 most influential compounds for differentiating exposed and healthy groups in the PLS-DA were annotated using HMDB, METLIN, and LIPIDMAPS databases. From metabolomics, the experiment was found to have no significative effect on zebrafish metabolism in 6 h experiments. For the 24 h experiment, the most relevant compounds belonged to 10 classes correlated to three biological mechanisms. The level changes in the annotation of three triglycerides and two diglycerides relate to the accumulation of lipids, resembling dyslipidemia in human patients and intrinsic inflammation. Also, two flavonoids and one triterpene saponin can correlate to this mechanism as anti-inflammatory agents being consumed or losing appetite during the experiment. A disorder in hormonal balance is present in changes observed in the decay of one isoprenoid, increase of one steroid ester and one sesquiterpenoid, and reflecting in one carotenoid decline. Also, annotating two sphingolipids and one ceramide could represent cell membrane adapting to mitigate protein infection. The LLLT was found to have an effect in lowering the lipid accumulation, with no signs of affecting the membrane changes, while partially regulating the hormonal disorder. The alterations observed suggest that the LLLT could help regulate back the metabolism of patients with COVID-19, speeding their recovery.

Title of the Event: X-Meeting / BSB 2023
City of the Event: Curitiba
Title of the Proceedings of the event: X-Meeting presentations
Name of the Publisher: Even3
Means of Dissemination: Meio Digital

How to cite

ALEXANDRE, Leonardo Santos et al.. STUDY OF METABOLIC CHANGES INDUCED BY SARS-COV-2 SPIKE PROTEIN IN ZEBRAFISH (DANIO RERIO) WITH LOW-LEVEL LASER AS ANTI-INFLAMMATORY THERAPY... In: X-Meeting presentations. Anais...Curitiba(PR) Campus da indústria, 2023. Available in: https//www.even3.com.br/anais/xmeeting2023/628142-STUDY-OF-METABOLIC-CHANGES-INDUCED-BY-SARS-COV-2-SPIKE-PROTEIN-IN-ZEBRAFISH-(DANIO-RERIO)-WITH-LOW-LEVEL-LASER-AS. Access in: 31/08/2025