X-Meeting presentations

PROBIOGENOMICS OF BACILLUS VELEZENSIS 1273: IN SILICO, IN VITRO, AND IN VIVO APPROACHES

Published in 21/11/2024 - ISBN: 978-65-272-0843-3

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Paper Title
PROBIOGENOMICS OF BACILLUS VELEZENSIS 1273: IN SILICO, IN VITRO, AND IN VIVO APPROACHES
Authors
  • Eduarda Guimarães Sousa
  • Gabriela Munis Campos
  • Beatriz Gamarano Rocha de Souza
  • Laura Diniz Freitas
  • Rafael Junio de Oliveira
  • Siomar de Castro Soares
  • Vasco A de C Azevedo
Modality
Poster
Subject area
DNA and Genomics
Publishing Date
21/11/2024
Country of Publishing
Brazil | Brasil
Language of Publishing
Inglês
Paper Page
https://www.even3.com.br/anais/xmeeting-2024/837048-probiogenomics-of-bacillus-velezensis-1273--in-silico-in-vitro-and-in-vivo-approaches
ISBN
978-65-272-0843-3
Keywords
Probiogenomic, Bacillus velezensis, probiotic
Summary
Bacillus velezensis is a Gram-positive, spore-forming bacterium that has well-described probiotic properties in vitro and in applications such as biocontrol against phytopathogens, in the food industry and in aquaculture research. Activities such as antimicrobial and antifungal by their secondary metabolites, genes that encode bioactive compounds like vitamins and genes related to immunomodulation, anti-inflammatory cytokine production are reported. Bacterial spore forms are potential targets for probiotics, as shown in studies using probiotics in the human diet for diseases and infections on the gastrointestinal tract, and other applications like in animal feeds because of their high capacity to survive in adverse conditions. There is no scientific evidence on the probiotic potential of this strain in vivo, as well as which proteins and molecular mechanisms would be related to its beneficial effects and its safety status for consumption. Analyses at the genomic level, combined with in vitro and in vivo studies, have been used to characterize new probiotic candidates, providing new perspectives on the main genetic factors and molecular mechanisms associated with the functional and safety characteristics of these microorganisms. Initially, Bacillus isolate were sequenced using the Illumina HiSeq platform, and the sample were assembled and annotated. To perform phylogenomic analysis DNA-DNA hybridization was performed, to verify the species of the isolate. Average Nucleotide Identity was used to identify similarities between homologous regions of two genomes, followed by the construction of a phylogenomic tree and pangenome analysis to elucidate the phylogenomic relationships between Bacillus strains, using bioinformatics tools. Thus, with the probiogenomic approach, this study characterized the probiotic profile of BAC1273 in vivo, using in a healthy animal model, the research indicated that it is a potential probiotic strain, showing no histopathological damage to the intestinal mucosa in healthy animal models. Genetic factors associated with anti-inflammatory activity have also been identified through comparative genomics in other probiotic strains of the species. In addition, this strain shows antibacterial activity against pathogenic bacteria in vitro, possibly through bacteriocins and the production of organic acids, whose genes were identified in silico. At the phenotypic level, the antimicrobial resistance of BAC1273 to antibiotics and genes associated with virulence and toxic metabolites were analyzed. The phenotypic assays also showed that BAC1273 survives acid stress. Furthermore, no adverse effects were observed in the clinical and histopathological analysis of healthy mice after consumption of the strain for 13 consecutive days. Overall, the findings of the present work show that BAC1273 presents beneficial functional characteristics in vivo, in silico and in vitro and revealed that this strain presents a certain level of safety to be exploited for consumption and future probiotic applications.
Title of the Event
20º Congresso Brasileiro de Bioinformática: X-Meeting 2024
City of the Event
Salvador
Title of the Proceedings of the event
X-Meeting presentations
Name of the Publisher
Even3
Means of Dissemination
Meio Digital

How to cite

SOUSA, Eduarda Guimarães et al.. PROBIOGENOMICS OF BACILLUS VELEZENSIS 1273: IN SILICO, IN VITRO, AND IN VIVO APPROACHES.. In: X-Meeting presentations. Anais...Salvador(BA) Hotel Deville Prime, 2024. Available in: https//www.even3.com.br/anais/xmeeting-2024/837048-PROBIOGENOMICS-OF-BACILLUS-VELEZENSIS-1273--IN-SILICO-IN-VITRO-AND-IN-VIVO-APPROACHES. Access in: 18/07/2025

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