COMPARATIVE ANALYSIS OF TRANSPOSABLE ELEMENTS IN THE TILAPIA (OREOCHROMIS NILOTICUS) GENOME: A CASE STUDY OF STREPTOCOCCUS AGALACTIAE INFECTION

Lorena Maria Rudnik; Katia Brumatti; laurival antonio vilas Boas; LUCIENNE GARCIA PRETTO GIORDANO; Jorge Manuel de Oliveira Fernandes; Renan José Cassaroto Appel; ALEXANDRE ROSSI PASCHOAL

Paper Title

Authors

Lorena Maria Rudnik
Katia Brumatti
laurival antonio vilas Boas
LUCIENNE GARCIA PRETTO GIORDANO
Jorge Manuel de Oliveira Fernandes
Renan José Cassaroto Appel
ALEXANDRE ROSSI PASCHOAL

Modality

Poster

Subject area

RNA and transcriptomics

Publishing Date

21/11/2024

Country of Publishing

Brazil | Brasil

Language of Publishing

Inglês

Paper Page

https://www.even3.com.br/anais/xmeeting-2024/832991-comparative-analysis-of-transposable-elements-in-the-tilapia-(oreochromis-niloticus)-genome--a-case-study-of-stre

ISBN

978-65-272-0843-3

Keywords

Tilapia, Transposable elements, streptococcosis, bioinformatics, gene expression, aquaculture

Summary

The Nile tilapia (Oreochromis niloticus) is one of the most cultivated fishes in the world. Various diseases can occur in intensive farming, but streptococcosis, caused mainly by Streptococcus agalactiae, is the most common in tilapia farming, with mortality rates reaching up to 70%. Many studies have been conducted to better understand the interaction between tilapia and the pathogen during the infection process, contributing to the management of streptococcosis.Transposable elements (TEs) can introduce variations in the genome, influencing the expression of genes potentially associated with the disease, affecting both disease progression and the susceptibility of the animal host. This study analyzed the differential expression of TEs in Nile tilapia comparing healthy (control) and infected with two different serotypes of S. agalactiae: strain E8-UEL, serotype Ib, and strain Maranhão, serotype III. TE annotation was performed through the RepeatMasker tool, followed by comparative analysis using Cdhit and GFFCompare tools to assess the genomic differences of the elements between groups. Subsequently, the TEtranscripts tool allowed the assessment of TEs expression patterns. Results revealed a total of 3793 TEs, with 1071 TEs annotated in the control group, 1323 TEs in animals infected with the E8-UEL strain, and 1399 TEs in animals infected with the Maranhão strain, all distributed across 34 superfamilies. Notably, differential expression analysis indicated potential impacts of TEs on host gene expression during the infection, with distinct TE expression profiles observed within the genomes. These findings underscore the potential role of TEs in host-pathogen interaction between tilapia and S. agalactiae, offering new perspectives for the development of disease control strategies in aquaculture.

Title of the Event: 20º Congresso Brasileiro de Bioinformática: X-Meeting 2024
City of the Event: Salvador
Title of the Proceedings of the event: X-Meeting presentations
Name of the Publisher: Even3
Means of Dissemination: Meio Digital

How to cite

RUDNIK, Lorena Maria et al.. COMPARATIVE ANALYSIS OF TRANSPOSABLE ELEMENTS IN THE TILAPIA (OREOCHROMIS NILOTICUS) GENOME: A CASE STUDY OF STREPTOCOCCUS AGALACTIAE INFECTION.. In: X-Meeting presentations. Anais...Salvador(BA) Hotel Deville Prime, 2024. Available in: https//www.even3.com.br/anais/xmeeting-2024/832991-COMPARATIVE-ANALYSIS-OF-TRANSPOSABLE-ELEMENTS-IN-THE-TILAPIA-(OREOCHROMIS-NILOTICUS)-GENOME--A-CASE-STUDY-OF-STRE. Access in: 07/08/2025