DESIGN OF A MULTI-EPITOPE CHIMERIC VACCINE TARGETING SURFACE PROTEINS OF LEISHMANIA INFANTUM

Davi Salles Xavier; João Marcelo Pereira Alves

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Submission

Paper Title

DESIGN OF A MULTI-EPITOPE CHIMERIC VACCINE TARGETING SURFACE PROTEINS OF LEISHMANIA INFANTUM

Authors

Davi Salles Xavier
João Marcelo Pereira Alves

Modality

Poster

Subject area

Proteins and Proteomics

Publishing Date

21/11/2024

Country of Publishing

Brazil | Brasil

Language of Publishing

Inglês

Paper Page

https://www.even3.com.br/anais/xmeeting-2024/832817-design-of-a-multi-epitope-chimeric-vaccine-targeting-surface-proteins-of-leishmania-infantum

ISBN

978-65-272-0843-3

Keywords

reverse vaccinology, multi-epitope vaccine, Leishmania infantum

Summary

Considering the relevance of surface proteins in parasitic development and direct interaction with the host organism, certain approaches are intended to utilize these components as potentiating vaccines for diseases of global importance. Some in silico screening strategies can facilitate the selection of these targets and circumvent the challenge of antigenic complexity, selecting targets that will be more easily recognized by the immune system (such as externalized proteins, for example) and construction of multi-epitope compounds. Leishmaniasis is a neglected pathology responsible for nearly two thousand new cases and between 20 and 30 thousand deaths annually. The visceral manifestation is characterized as a more severe form of the disease and is caused mainly by L. infantum, which still does not have a well-established therapeutic or vaginal model. That said, this project presents a reverse vaccinology pipeline for initial screening of protein targets with vaccine potential and subsequent submission of the proteins found to epitope prediction for the construction of a multi-epitope chimeric protein. For this, the proteome of Leishmania infantum (UP000008153) was selected from the UniProt database, and submitted to a succession of tools aimed at selecting (i) surface or extracellular proteins (predicted by DeepLoc), (ii) wide distribution of orthologs (performed by OrthoFinder), which shows (iii) low similarity with the host (performed by BLASTp), and which have (iv) negative prediction for allergenicity (performed by AlgPred 2.0) and (v) positive for antigenicity (performed by VaxiJen). Seven proteins were identified and some of them have already been experimentally associated with essential functions in the survival of the parasite and in establishing the relationship with the host cells, according to the literature. Others are hypothetical proteins that may have novel vaccine applicability. The construction of the chimera was carried out initially by submitting the proteins found for signal peptide cleavage (performed by SignalP 6.0) and subsequent prediction of lymphocyte epitopes TCD4+ (performed by netMHCII 2.3), TCD8+ (performed by netCTL 1.2) and B (performed by BepiPred 2.0). The ideal chimeric configuration is still being standardized, exploring some physicochemical parameters of different linkers, adjuvants and epitope arrangement.

Title of the Event: 20º Congresso Brasileiro de Bioinformática: X-Meeting 2024
City of the Event: Salvador
Title of the Proceedings of the event: X-Meeting presentations
Name of the Publisher: Even3
Means of Dissemination: Meio Digital

How to cite

XAVIER, Davi Salles; ALVES, João Marcelo Pereira. DESIGN OF A MULTI-EPITOPE CHIMERIC VACCINE TARGETING SURFACE PROTEINS OF LEISHMANIA INFANTUM.. In: X-Meeting presentations. Anais...Salvador(BA) Hotel Deville Prime, 2024. Available in: https//www.even3.com.br/anais/xmeeting-2024/832817-DESIGN-OF-A-MULTI-EPITOPE-CHIMERIC-VACCINE-TARGETING-SURFACE-PROTEINS-OF-LEISHMANIA-INFANTUM. Access in: 21/05/2025