ALTERED INTERCELLULAR COMMUNICATION IN RESPONSE TO HIGH AMYLOID BUILD-UP

Loren Dos Santos; Roberto Tadeu Raittz; Katia de Paiva Lopes

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Paper Title

ALTERED INTERCELLULAR COMMUNICATION IN RESPONSE TO HIGH AMYLOID BUILD-UP

Authors

Loren Dos Santos
Roberto Tadeu Raittz
Katia de Paiva Lopes

Modality

Poster

Subject area

RNA and transcriptomics

Publishing Date

21/11/2024

Country of Publishing

Brazil | Brasil

Language of Publishing

Inglês

Paper Page

https://www.even3.com.br/anais/xmeeting-2024/831451-altered-intercellular-communication-in-response-to-high-amyloid-build-up

ISBN

978-65-272-0843-3

Keywords

cell-cell communication, single-nuclei RNASeq, dementia, Alzheimer's Disease, Human brain

Summary

The pathology of Alzheimer's disease (AD) is characterized by the accumulation of ß-amyloid (Aß) plaques and neurofibrillary tangles caused by hyperphosphorylated tau. AD is the main cause of dementia, a condition that is estimated to affect 55 million worldwide. It is a progressive disorder that causes brain atrophy and cell degeneration causing memory loss and confusion. In every human brain, there are physiological processes involving intercellular communications that can occur through autocrine, paracrine, juxtacrine and endocrine connections, which involve ligands and receptor complexes. These ligand-receptor complexes activate or inhibit the expression of specific pathways responsible for events within and between cells in healthy and diseased states. Therefore, understanding the intercellular communication in the human brain is essential to explore the potential mechanisms behind synaptic loss, a hallmark in neurodegenerative diseases. Here, we leveraged single-nuclei RNASeq (snRNASeq) data obtained from postmortem human brain tissues of 48 individuals from the Religious Orders Study (ROS) and the Rush Memory and Aging Project (MAP). The data was previously annotated into eight major cell-type groups, namely: Astrocytes, Endothelial Cells, Excitatory and Inhibitory Neurons, Microglia, Oligodendrocytes, Oligodendrocyte precursor cells and Pericytes. For the cell-communication analysis, we focused on the low and high amyloid groups (35,140 and 35,494 cells, respectively) and ran the CellChat pipeline. Preliminary results indicate differences between these two groups. High Aß group showed an increase in cell-cell links of 80% in Microglia, 31% in Endothelial cells, and 17% in Oligodendrocytes compared to low Aß. On the other hand, there was a decrease of 42.2% of connections in Excitatory Neurons in high Aß compared to low Aß (187 vs 108 total, respectively). The results provide evidence that different cell communications occur when the brain is challenged, opening new roads for future analysis. Our next approach will incorporate the tau information and perform the cell communication analysis for a broad spectrum of the disease. We will also associate the ligand-receptor pairs with a measure of longitudinal cognitive decline to capture the different interactions throughout a patient's life.

Title of the Event: 20º Congresso Brasileiro de Bioinformática: X-Meeting 2024
City of the Event: Salvador
Title of the Proceedings of the event: X-Meeting presentations
Name of the Publisher: Even3
Means of Dissemination: Meio Digital

How to cite

SANTOS, Loren Dos; RAITTZ, Roberto Tadeu; LOPES, Katia de Paiva. ALTERED INTERCELLULAR COMMUNICATION IN RESPONSE TO HIGH AMYLOID BUILD-UP.. In: X-Meeting presentations. Anais...Salvador(BA) Hotel Deville Prime, 2024. Available in: https//www.even3.com.br/anais/xmeeting-2024/831451-ALTERED-INTERCELLULAR-COMMUNICATION-IN-RESPONSE-TO-HIGH-AMYLOID-BUILD-UP. Access in: 19/07/2025