X-Meeting presentations

MICROBIOTA DIVERSITY AND ASSOCIATION WITH PERFORMANCE PHENOTYPES IN YOUNG NELORE BULLS

Published in 21/11/2024 - ISBN: 978-65-272-0843-3

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Paper Title
MICROBIOTA DIVERSITY AND ASSOCIATION WITH PERFORMANCE PHENOTYPES IN YOUNG NELORE BULLS
Authors
  • Liz Jiannine Fuentes Rojas
  • Tainã Figueiredo Cardoso
  • Gabriel Alexander Colmenarez Pena
  • Wanderson Anjos da Silva
  • Bárbara Cunha Padilha Antonio
  • Liliane Conteville
  • Fernando De Oliveira Bussiman
  • Daniela Andressa Lino Lourenço
  • Lauro Fraga Almeida
  • Henrique Torres Ventura
  • Luiz Antônio Josahkian
  • Juliana Jorge Paschoal
  • Gerson Barreto Mourão
  • Luciana Correia De Almeida Regitano
Modality
Poster
Subject area
DNA and Genomics
Publishing Date
21/11/2024
Country of Publishing
Brazil | Brasil
Language of Publishing
Inglês
Paper Page
https://www.even3.com.br/anais/xmeeting-2024/831261-microbiota-diversity-and-association-with-performance-phenotypes-in-young-nelore-bulls
ISBN
978-65-272-0843-3
Keywords
Association, Metabarcoding, Microbiota diversity, Nelore bulls, Performance phenotypes
Summary
The bovine gastrointestinal tract is an ecosystem with a complex and diverse microbial community responsible for the overall health of the animals, being associated with their digestion and immunology. Next-generation sequencing studies using 16S rRNA sequencing describe the composition of the microbiota, which may be used in association with different animal performance phenotypes. This study aimed to explore the fecal microbiota of 475 Nelore bulls aged between 18 and 30 months, who participated in the 2019 to 2022 editions of the National Program for the Evaluation of Young Bulls (PNAT). Fecal samples were collected from the rectal ampulla of Nelore bulls and DNA extraction was performed from 150 mg of feces using the Quick-DNA ™ Fecal/Soil Microbe Miniprep Kit (ZYMO Research Corp). PCR target amplification for 16S rRNA was performed with designed primers and the amplicons were sequenced on an Illumina NovaSeq platform (2 × 250 bp). After sequencing, the raw reads were filtered for quality (>Q25) and trimmed at positions 250 (F) and 250 (R) using QIIME 2 v.2022.8. The filtered data was submitted to the DADA2 package to generate amplicon sequence variants (ASVs) and the sequences were annotated using the SILVA v.138.1 database. The resulting ASV table was used to determine alpha diversity (number of ASVs and the Shannon-Wiener index), beta diversity (Unweighted UniFrac distance) and to assess their association with performance phenotypes, using the linear mixed model implemented in the lme4qtl R package. We identi?ed a total of 86.780 bacterial ASVs and 3.621 archaeal ASVs in the young Nelore bulls’ microbiomes. Based on the alpha diversity, we could observe a large intra-year diversity considering both bacteria and archaeal populations. When comparing the Shannon index between each pair of years, we observed that all four groups were significantly different (p <= 0.05, Wilcoxon test) from each other regarding bacterial diversity, but that there were no significant differences regarding archaeal diversity. Regarding beta diversity, we observed no significant clustering of samples from each year, neither in bacteria nor in archaea. In total, five positive (for Gross feed efficiency, Gross feed intake, Observed dry matter intake, Average daily gain, and Average metabolic liveweight) and five negative (for Gross feed efficiency, Gross feed intake, Observed dry matter intake, Average daily gain, and Average metabolic liveweight) associations were found between bacterial ASVs at the family level and phenotypes. In archaea, three positive (for Average live weight, Average metabolic live weight, Average daily gain) associations were found between archaean ASVs at genus level and phenotypes. Further Genome Wide Association Analyses will be carried out, as well as estimates of heritability and genetic predictions considering microbiome parameters.
Title of the Event
20º Congresso Brasileiro de Bioinformática: X-Meeting 2024
City of the Event
Salvador
Title of the Proceedings of the event
X-Meeting presentations
Name of the Publisher
Even3
Means of Dissemination
Meio Digital

How to cite

ROJAS, Liz Jiannine Fuentes et al.. MICROBIOTA DIVERSITY AND ASSOCIATION WITH PERFORMANCE PHENOTYPES IN YOUNG NELORE BULLS.. In: X-Meeting presentations. Anais...Salvador(BA) Hotel Deville Prime, 2024. Available in: https//www.even3.com.br/anais/xmeeting-2024/831261-MICROBIOTA-DIVERSITY-AND-ASSOCIATION-WITH-PERFORMANCE-PHENOTYPES-IN-YOUNG-NELORE-BULLS. Access in: 06/05/2025

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