Proceedings of the XV Latin American Workshop and Symposium on Anaerobic Digestion: Contributing to the Energy Transition and Decarbonization

INFLUENCE OF CLIMATE AND DIET ON METHANE POTENTIAL FROM DAIRY MANURE IN AND OUT OF LACTATION

Published in 11/12/2025 - ISSN: -

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Paper Title
INFLUENCE OF CLIMATE AND DIET ON METHANE POTENTIAL FROM DAIRY MANURE IN AND OUT OF LACTATION
Authors
  • Marcus Lívio Carlin
  • Jéssica Jacinta Silva
  • Renata Piacentini Rodriguez
Modality
Artigo
Subject area
Post-treatment and conditioning of anaerobic effluents, biogas and digestate
Publishing Date
11/12/2025
Country of Publishing
Brazil | Brasil
Language of Publishing
en-US
Paper Page
https://www.even3.com.br/anais/workshop-simposio-digestao-anaerobia/1180151-influence-of-climate-and-diet-on-methane-potential-from-dairy-manure-in-and-out-of-lactation
ISSN
-
Keywords
Keywords: biogas; anaerobic digestion; energy potential; cattle manure; livestock.
Summary
INFLUENCE OF CLIMATE AND DIET ON THE METHANE POTENTIAL OF DAIRY MANURE DURING AND OUTSIDE LACTATION Marcus L. Carlin 1 *, Jessica J. Silva 1 & Renata P. Rodriguez 2 1 Aluno de doutorado / Ciências Ambientais / Instituto de Ciência e Tecnologia/ Universidade Federal de Alfenas/Poços de Caldas, Minas Gerais, Brasil. 2 Professor Doutor/ Instituto de Ciência e Tecnologia/ Universidade Federal de Alfenas/Poços de Caldas, Minas Gerais, Brasil . *Correspondente endereço de e-mail do autor: marcus.carlin@sou.unifal-mg.edu.br Highlights • Manure from lactating cows produces more methane due to more digestible and energy-rich diets. • Seasonal climate influences methane production by altering feed quality and animal metabolism. • Winter samples from non-lactating cows showed the lowest methane production potential. The intensification of dairy cattle production systems is a consolidated reality with a continued upward trend. The goal is to maximize animal efficiency in smaller areas. One consequence of this intensification is the accumulation of waste in increasingly smaller spaces, making it essential to implement techniques that can mitigate the resulting environmental impact. The improvement of these techniques should avoid empirical practice without well-founded theoretical criteria, as the incorrect use of this waste can contribute to the contamination of surface water resources and soil and groundwater pollution. The energy use of cattle manure through biogas production is a well-established technique for managing this material, contributing to the generation of energy in the form of methane gas. However, it is essential that biogas and biomethane production systems exhibit minimal variations due to substrate characteristics. Due to Brazil's continental size, beef and dairy cattle management practices vary throughout the year, especially due to diverse climatic conditions. In this context, this project aimed to evaluate the Biochemical Methane Potential (BMP) of dairy cattle manure at different times of the year. Four cattle manure samples from dairy cattle were collected in the winter, spring, and summer of 2023 and fall of 2024. The substrates (lactation and non-lactation manure samples) were characterized for solids content according to Standard Methods for the Examination of Water and Wastewater (APHA, 2012). Analyses of crude protein, ether extract, crude fiber, mineral salts, and moisture were performed by a certified laboratory. The inoculum (anaerobic granular sludge) came from an upflow reactor. Anaerobic Sludge Blanket (UASB) treating poultry slaughterhouse effluent (Avícola Ideal, Pereiras-SP), following the same substrate solids characterization method. The chemical analyses of cattle feces, corn silage, which represents the roughage fraction of the L diet, and forage ( Brachiaria brizantha ), the latter representing the NL diet, were also analyzed by a certified laboratory. Fecal samples were analyzed in their original physical state, called "wet sample," and after drying in an oven at 60°C for 16 to 24 hours or until they reached a constant weight (variation of less than 0.1% for two successive weighings), called a dry sample. The experiments were performed in mono-digestion systems using the inoculum and one of the bovine manure substrates in each climatic season. A total of 125 ml of useful volume was added to each test, with a headspace volume of 125 ml intended for biogas accumulation. The biomass concentration inside each flask was defined according to the VDI4630 methodology, which recommends performing biochemical potential of methane (BMP) tests in a 2:1 ratio in terms of total volatile solids (VS) for the inoculum and substrate, respectively, according to Deutscher (2006). The BMP tests were operated at 35°C with 20 rpm agitation using a refrigerated shaker incubator . The trials carried out with substrates from the collections lasted 130, 147, 133 and 113 days, in summer, autumn, winter and spring, with an average accumulated biogas volume of 2150 ml, 1185 ml, 1350 ml and 2585 ml, for the trials with manure from L cows and 1650 ml, 1335 ml, 860 ml, 1812 ml, for manure from non-NL cows, respectively. The specific methane production (PEM) was 407, 122, 218, 219 ml NCH 4 /g SV, for the L diets and 389, 145, 185, 208 ml NCH 4 /g SV for the NL diets in summer, autumn, winter and spring, respectively. These results suggest a significant influence of diet and seasonality on PBM assays. Regarding diet, the influence occurs primarily from the difference in digestibility between feeds. Regarding seasonality , the influence arises from changes in metabolism and behavior, particularly ingestive behavior , in response to seasonal climate variations in order to maintain homeothermy . Both dietary and metabolic variations, along with changes in digestive behavior, will define the physical/chemical composition of excreted manure and its qualitative and quantitative potential for biogas and methane production. Therefore, it is imperative to evaluate the interactions between seasonal variations and management practices and their consequences for biogas production from cattle manure, aiming to optimize biogas and biomethane production systems in Brazil.
Title of the Event
XV Latin American Workshop and Symposium on Anaerobic Digestion: Contributing to the energy transition and decarbonization
City of the Event
Fortaleza
Title of the Proceedings of the event
Proceedings of the XV Latin American Workshop and Symposium on Anaerobic Digestion: Contributing to the Energy Transition and Decarbonization
Name of the Publisher
Even3
Means of Dissemination
Meio Digital

How to cite

CARLIN, Marcus Lívio; SILVA, Jéssica Jacinta; RODRIGUEZ, Renata Piacentini. INFLUENCE OF CLIMATE AND DIET ON METHANE POTENTIAL FROM DAIRY MANURE IN AND OUT OF LACTATION.. In: Proceedings of the XV Latin American Workshop and Symposium on Anaerobic Digestion: Contributing to the Energy Transition and Decarbonization. Anais...Fortaleza (CE) Even3, UFC, Embrapa, 2025. Available in: https//www.even3.com.br/anais/workshop-simposio-digestao-anaerobia/1180151-INFLUENCE-OF-CLIMATE-AND-DIET-ON-METHANE-POTENTIAL-FROM-DAIRY-MANURE-IN-AND-OUT-OF-LACTATION. Access in: 07/05/2026

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