INTERACTION OF GINGKO BILOBA EXTRACT WITH AMYLOID BETA PROTEIN IN POPC MONOLAYERS

Juliana Picariello Gava; Lucineia F Ceridorio

Todos os Trabalhos

Trabalho

Título do Trabalho

INTERACTION OF GINGKO BILOBA EXTRACT WITH AMYLOID BETA PROTEIN IN POPC MONOLAYERS

Autores

Juliana Picariello Gava
Lucineia F Ceridorio

Modalidade

Pôster

Área temática

3) Design, Synthesis, and Characterization of Electronic/Optoelectronic Materials and Devices

Data de Publicação

17/05/2024

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/workshop-do-ineo-2024/796777-interaction-of-gingko-biloba-extract-with-amyloid-beta-protein-in-popc-monolayers

ISBN

978-65-272-0462-6

Palavras-Chave

Alzheimer's disease, beta-amyloid, Ginkgo Biloba, Langmuir monolayers

Resumo

Juliana Gava (IC) e Lucineia F. Ceridorio (Pq) Universidade Federal de São Paulo -Campus Diadema Departamento de Química Alzheimer's disease (AD) is a dementia syndrome characterized by functional and cognitive decline. It has a high prevalence in the elderly. One of the brain changes associated with this neurodegenerative disease is the clustering of Amyloid beta (Aß), a peptide produced by abnormal cleavage of the amyloid precursor protein (APP). Amyloid precursor protein is found in small quantities in lipid membranes in the brain. The continuous synthesis of the protein causes an accumulation in the organism, which can result in intraneural neurofibrillary tangles and senile plaques distributed in the cerebral cortex. The result of this accumulation is the death of neurons due to a lack of vascularization and activation of the immune system, which begins to attack healthy cells, causing memory loss and mental confusion. Studies aimed at reversing or preventing the accumulation of amyloid- ß have been carried out using the extract of the Ginkgo Biloba plant (EGB 761) and have shown that it prevents the production and toxicity of Amyloid-ß , thus providing benefits in the treatment of Alzheimer's disease. In this context, the aim of this study is to analyze the adsorption of Amyloid- ß protein on Langmuir monolayers of POPC, phospholipid 1-palmitoyl-2-oleoyl phosphatidylcholine (POPC) in the presence of ginkgo biloba extract (EGB 761) at the interface of phosphate-buffered saline (PBS). For this analysis, surface pressure isotherms, kinetics, hysteresis and Brewster's angle micrographs were carried out. The results showed that different volumes of EGB at the interface (from 100 to 500 uL) had similar changes in the isotherms, indicating that the interaction is not volume dependent. In the POPC with Aß monolayer isotherm, there is a decrease in the surface pressure in the larger molecular areas, such as relaxation of the monolayer, starting at 10 mN/m. The presence of the Ginkgo biloba extract causes the expansion of the liquid region. When the surface pressure reaches about 15 mN/m, the molecular area decreases, resulting in the condensation of the solid area of the POPC/Aß- monolayer, indicating that the EGB is interacting with the Amyloid- ß protein. Analysis of the isotherms shows that EGB only has a significant effect only on the mixed monolayer (POPC and AB), indicating that EGB interacts directly with the Aß protein. In addition, Brewster's angle microscopy (BAM) images show the formation of EGB conglomerates in the POPC monolayer, This conglomerates disappear with the Aß protein in the subphase, due to the interaction between EGB and Aß, which causes the EGB molecules to move apart and become more uniformly distributed in the interface. The results of this study corroborate those in vivo studies related to the improvement of Alzheimer's disease with the use of GBE by demonstrating the effect of EGB in the POPC- Aß monolayer.

Título do Evento: Workshop do INEO 2024
Cidade do Evento: Nazaré Paulista
Título dos Anais do Evento: Anais do Workshop INEO 2024
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

GAVA, Juliana Picariello; CERIDORIO, Lucineia F. INTERACTION OF GINGKO BILOBA EXTRACT WITH AMYLOID BETA PROTEIN IN POPC MONOLAYERS.. In: Anais do Workshop INEO 2024. Anais...Nazaré Paulista(SP) Hotel Estância Atibainha, 2024. Disponível em: https//www.even3.com.br/anais/workshop-do-ineo-2024/796777-INTERACTION-OF-GINGKO-BILOBA-EXTRACT-WITH-AMYLOID-BETA-PROTEIN-IN-POPC-MONOLAYERS. Acesso em: 16/05/2025