INTEGRATIVE TAXONOMY OF CRUZIA TENTACULATA, RUDOLPHI,1819 (NEMATODA: KATHLANIIDAE), IN WHITE-EARED OPOSSUMS (DIDELPHIS ALBIVENTRIS), IN URBAN AREAS OF CAMPO GRANDE, MATO GROSSO DO SUL

Ana Clara da Costa Teixeira; Emilly Rebeca Antunes Gonçalves; Isabela Caroline Oliveira da Silva; Carina Elisei de Oliveira

Título do Trabalho

Autores

Ana Clara da Costa Teixeira
Emilly Rebeca Antunes Gonçalves
Isabela Caroline Oliveira da Silva
Carina Elisei de Oliveira

Modalidade

Resumo

Área temática

Genética de Microrganismos

Data de Publicação

03/09/2025

País da Publicação

Brasil

Idioma da Publicação

Português

Página do Trabalho

https://www.even3.com.br/anais/vicgco/1221834-integrative-taxonomy-of-cruzia-tentaculata-rudolphi1819-(nematoda--kathlaniidae)-in-white-eared-opossums-(did

ISBN

978-65-272-1473-1

Palavras-Chave

DNA Barcoding, cox-1 gene, helminth

Resumo

Cruzia tentaculata is a nematode frequently found in marsupials, exhibiting high prevalence and abundance, and is considered an endemic parasite of South America. The identification of helminths in urban wildlife is essential for understanding parasite biodiversity and its ecological implications. This study aimed to perform the morphological and molecular identification, as well as to infer the phylogenetic position of C. tentaculata parasitizing Didelphis albiventris in urban areas of Campo Grande, MS. Twenty-three specimens of white-eared opossums (D. albiventris) were necropsied, with inspection of the small and large intestines for parasite collection. The helminths were fixed in 70% ethanol, cleared with diluted glycerin, mounted on slides, and identified based on specialized literature. Genomic DNA was extracted using the QIAamp Blood DNA Mini Kit (Qiagen), and the mitochondrial cox-1 gene was amplified by PCR with the COX1A primer. The sequences obtained via Sanger sequencing were edited in BioEdit, assembled in CAP3, and compared by BLAST, showing 97–98% identity and 98% coverage with sequences deposited in GenBank® (OM670378.1, OM670305.1, OR713897.1, OM670352.1). The phylogenetic analysis by maximum likelihood (500 bootstraps), conducted in MEGA11, placed C. tentaculata in a monophyletic clade, distinct from the outgroups (T. cati and A. lumbricoides), confirming its molecular identity. The observed prevalence was 74%, with a mean abundance of 617.22 and a mean intensity of 831.88. It is concluded that the cox-1 gene is an efficient marker for the molecular identification of helminths, and that DNA barcoding proves to be a precise tool for studies of taxonomy and genetic diversity in wildlife parasites.

Título do Evento: VI Congresso de Genética do Centro-Oeste
Cidade do Evento: Campo Grande
Título dos Anais do Evento: Congresso de Genética do Centro-Oeste
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

TEIXEIRA, Ana Clara da Costa et al.. INTEGRATIVE TAXONOMY OF CRUZIA TENTACULATA, RUDOLPHI,1819 (NEMATODA: KATHLANIIDAE), IN WHITE-EARED OPOSSUMS (DIDELPHIS ALBIVENTRIS), IN URBAN AREAS OF CAMPO GRANDE, MATO GROSSO DO SUL.. In: Congresso de Genética do Centro-Oeste. Anais...Campo Grande(MS) UFMS, 2025. Disponível em: https//www.even3.com.br/anais/vicgco/1221834-INTEGRATIVE-TAXONOMY-OF-CRUZIA-TENTACULATA-RUDOLPHI1819-(NEMATODA--KATHLANIIDAE)-IN-WHITE-EARED-OPOSSUMS-(DID. Acesso em: 15/10/2025