Annals of the 1st MCAA Brazil-Europe Workshop

TITYUS SERRULATUS (SCORPION) VENOM INDUCES REACTIVE OXYGEN SPECIES PRODUCTION BY RAW MACROPHAGES

Published in 15/05/2018 - ISBN: 978-85-5722-068-3

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Paper Title
TITYUS SERRULATUS (SCORPION) VENOM INDUCES REACTIVE OXYGEN SPECIES PRODUCTION BY RAW MACROPHAGES
Authors
  • Kelly Portela Sousa
  • Lillian Nunes Gomes
  • ANDRÉ ALVARES MARQUES VALE
  • Carla Regina Feitosa Grosse de Andrade
  • Lais Couto e Lima Alencar
  • Eder Fialho
  • Ângela Falcai
  • Paulo Vítor Soeiro Pereira
Modality
resumo
Subject area
Biological Sciences
Publishing Date
15/05/2018
Country of Publishing
Brasil
Language of Publishing
Português
Paper Page
https://www.even3.com.br/anais/mcaaworkshop/61214-tityus-serrulatus-(scorpion)-venom-induces-reactive-oxygen-species-production-by-raw-macrophages
ISBN
978-85-5722-068-3
Keywords
Venon, Inflamation, Macrophage, Oxide Burst
Summary
Tityus serrulatus (scorpion) venom induces Reactive Oxygen Species production by RAW macrophages Introduction: Macrophages present an essential role against pathogens. Reactive oxygen species (ROS) produced by those cells controls several infections, since hydrogen peroxide, for example, has an important microbicidal and inflammatory function. In this work, we evaluated the effect of Tityus serrulatus venom (Tsv) on H2O2 production. Material and Methods: RAW 264.7 cells (1 x 106 / mL) were incubated with T. serrulatus venom (Tsv - 0.1, 1.0, 10.0 µg / mL) and / or LPS (10 µg / mL), for 24 or 48 hours. After treatments, cells were incubated with DHR (375 ng / mL) for 10 minutes to proceed H2O2 production assay on the flow cytometer. The experiments were performed in triplicate, and the one-way ANOVA test was applied, (p < 0.05). Results and Discussion: An increase in oxidative burst is an important feature of activated macrophages. The treatment with Tsv was able to induce H2O2 production, presenting significant values for all tested concentrations, both in 24 and 48 hours. However, Tsv / LPS associated treatment presented lower oxidative response, when compared to Tsv-stimulated groups, even this reduction was not statistically significant. Conclusion: T. serrulatus venom was able to activate macrophages by increasing ROS production. Thus, this work opens new perspectives for the use of T. serrulatus venom, which may lead to the development of new therapies for infections control. Acknowledgments: The Federal University of Maranhão, the Immunophysiology Laboratory and the development agencies CAPES and FAPEMA.
Title of the Event
1st MCAA Brazil-Europe Workshop (BREUW): Building a sustainable future based on cooperative science, technology and education
City of the Event
São Luís
Title of the Proceedings of the event
Annals of the 1st MCAA Brazil-Europe Workshop
Name of the Publisher
Even3
Means of Dissemination
Meio Digital

How to cite

SOUSA, Kelly Portela et al.. TITYUS SERRULATUS (SCORPION) VENOM INDUCES REACTIVE OXYGEN SPECIES PRODUCTION BY RAW MACROPHAGES.. In: Annals of the 1st MCAA Brazil-Europe Workshop. Anais...São Luís(MA) UFMA, 2018. Available in: https//www.even3.com.br/anais/mcaaworkshop/61214-TITYUS-SERRULATUS-(SCORPION)-VENOM-INDUCES-REACTIVE-OXYGEN-SPECIES-PRODUCTION-BY-RAW-MACROPHAGES. Access in: 08/09/2025

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