DIFFERENTIAL EXPRESSION OF IMMUNE GENES IN LARVAE, NYMPHS AND ADULTS OF RHIPICEPHALUS MICROPLUS IN RESPONSE TO THEILERIA EQUI INFECTION.

Carla Alves Rabello; Karina Cristina Paes Galdino; Nelson Meireles da Silva; Pamella Pryscila De Alvarenga Bissoli Maciel De Lima; Patrícia Gonzaga Paulino; Cristiane De Medeiros Campanha; Laís Feliciano de Souza; Huarrisson Azevedo Santos

Título do Trabalho

Autores

Carla Alves Rabello
Karina Cristina Paes Galdino
Nelson Meireles da Silva
Pamella Pryscila De Alvarenga Bissoli Maciel De Lima
Patrícia Gonzaga Paulino
Cristiane De Medeiros Campanha
Laís Feliciano de Souza
Huarrisson Azevedo Santos

Modalidade

Resumo

Área temática

Protozoários e Agentes transmitidos por vetores de importância em Saúde

Data de Publicação

10/03/2025

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/ix-simposio-internacional-em-ciencias-veterinarias-sincvet-ix-international-symposium-in-veterinary-sciences-isvs-499466/1031244-differential-expression-of-immune-genes-in-larvae-nymphs-and-adults-of-rhipicephalus-microplus-in-response-to-t

ISBN

978-65-272-1246-1

Palavras-Chave

piroplasmosis; Theileria equi; Rhipicephalus microplus; redox metabolism; innate immune pathways

Resumo

Equine piroplasmosis is a disease that affects horses in general and one of its causative agents is the protozoan Theileria equi. The pathogen is transmitted through the blood of infected ticks on susceptible hosts. In Brazil, Rhipicephalus (Boophilus) microplus is the only tick species with experimentally proven vectorial capacity; however, information on the T. equi-R. microplus interaction is still scarce. It is known that the vectorial competence of ticks is closely related to the ability of pathogens to escape the tick's defense mechanisms. As a result, the acquired pathogen needs to overcome various tissue barriers present in the tick's body in order to complete its cycle, such as the midgut, salivary gland and ovary. The innate immune response present in these tissues corresponds to the tick's main form of defense against infection, including the metabolism of reduced oxide, the complement system and the activity of phagocytic cells, which are mechanisms that need to be circumvented for the pathogen to survive in the vector's organism. Thus, knowledge about the molecular and cellular interaction at the tick-pathogen interface can provide new targets for blocking metabolic pathways that are fundamental for the infection, multiplication and persistence of T. equi in the tick, thus making it possible to block the transmission of this pathogen. Thus, The aim of this study is to use gene expression assays to evaluate the differential expression of genes involved in the main immune signaling pathways and in the oxidoreduction metabolism of R. microplus in response to infection by T. equi. To this end, pathogen-free ticks will be fed to a chronically infected horse and another uninfected horse, forming the infected and control groups. The larvae, nymphs and engorged females of each group will be collected directly from the animals' bodies and then sanitized in an alcohol and hypochlorite solution. The larvae and nymphs will be stored whole in RNAlater solution, while the females will be dissected in order to collect the intestine, salivary gland and ovary, which will also be stored individually in RNAlater and stored at -80º. RNA will then be extracted from each sample individually using a commercial kit, followed by the differential gene expression assay, which will analyze 14 genes related to redox metabolism and 8 related to immune signaling pathways, with subsequent statistical analysis to assess relevance. As piroplasmosis is a disease responsible for causing major damage to the health and economy of the equestrian sector in Brazil, and current treatment has proved ineffective in eliminating the parasite, understanding the interaction between T. equi-R. microplus provides support for the development of alternative measures to combat the disease and promote the health of brazilian's equine herd.

Título do Evento: IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS)
Título dos Anais do Evento: Anais do IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS)
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

RABELLO, Carla Alves et al.. DIFFERENTIAL EXPRESSION OF IMMUNE GENES IN LARVAE, NYMPHS AND ADULTS OF RHIPICEPHALUS MICROPLUS IN RESPONSE TO THEILERIA EQUI INFECTION... In: Anais do IX Simpósio Internacional em Ciências Veterinárias (SINCVET) / IX International Symposium in Veterinary Sciences (ISVS). Anais...Seropédica(RJ) UFRRJ, 2025. Disponível em: https//www.even3.com.br/anais/ix-simposio-internacional-em-ciencias-veterinarias-sincvet-ix-international-symposium-in-veterinary-sciences-isvs-499466/1031244-DIFFERENTIAL-EXPRESSION-OF-IMMUNE-GENES-IN-LARVAE-NYMPHS-AND-ADULTS-OF-RHIPICEPHALUS-MICROPLUS-IN-RESPONSE-TO-T. Acesso em: 14/07/2025