Annals of the International Symposium on Fungal Stress – ISFUS

APPLICATION OF PROBIOTICS FORMULATIONS TO PREVENT ORAL CANDIDIASIS: IN VITRO AND IN VIVO STUDY

Published in 15/05/2019 - ISBN: 978-85-5722-210-6

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Paper Title
APPLICATION OF PROBIOTICS FORMULATIONS TO PREVENT ORAL CANDIDIASIS: IN VITRO AND IN VIVO STUDY
Authors
  • Felipe de Camargo Ribeiro
  • Jéssica Diane dos Santos
  • Rodnei Dennis Rossoni
  • Patrícia Pimentel de Barros
  • Shashank Shukla
  • Anita Shukla
  • Helen Elizabeth Burgwyn Fuchs
  • Eleftherios E. Mylonakis
  • Juliana Campos Junqueira
Modality
Abstract
Subject area
Stress in fungal pathogenesis
Publishing Date
15/05/2019
Country of Publishing
Brasil
Language of Publishing
Inglês
Paper Page
https://www.even3.com.br/anais/isfus/159431-application-of-probiotics-formulations-to-prevent-oral-candidiasis--in-vitro-and-in-vivo-study
ISBN
978-85-5722-210-6
Keywords
Lactobacillus, C. albicans, Gellan Gum, Oral candidiasis, Probiotic formulations
Summary
Probiotic encapsulation technology is an emerging field that has the challenges of maintaining the viability of probiotic cells in commercial products, as well as providing controlled release that result in the inhibition of pathogens, such as Candida spp. Candidiasis is a major fungal infection of the oral cavity and the increased antifungal resistance has led to the search for alternative antimicrobial treatments. In this context, the objective of this work was to develop probiotic formulations by encapsulating Lactobacillus paracasei 28.4 in gellan gum, aiming its application in the control of oral candidosis. After preparation of the probiotic formulations at various concentrations of gellan gum (0.5-1% w / v), cell viability monitoring under different storage conditions (4°C and room temperature) and release systems (PBS or saliva) for 7 days was evaluated. The ability of formulations to inhibit C. albicans was analyzed in vitro and in the oral candida model in mice. After analyzing the data (ANOVA and Tukey's test), it was verified that L. paracasei 28.4 remained viable in all gellan gum formulations, showing a 1.1-1.4-fold decrease of L. paracasei 28.4 viability. In addition, there was greater release of Lactobacillus when the formulations were in contact with artificial saliva (2.53x106 CFU/mL) in relation to PBS (5.33x103 CFU/mL, p<0.001). All formulations were able to inhibit in vitro the growth, biofilm and filamentation of C. albicans. In the in vivo study, only the formulation of 1% can promote colonization of L. paracasei 28.4 in the oral cavity of the mice. Consequently, only this concentration was tested in oral candidiasis, reducing approximately 3 logs of C. albicans CFU/mL when compared with the control group (C. albicans). Therefore, gellan gum presents potential as a biomaterial for probiotics encapsulation in the development of products for oral candidosis control, and the formulation of 1% w/v showed promising results, since this formulation was able to promote the colonization of L. paracasei 28.4 in the mice oral cavity, reducing C. albicans in oral infection.
Title of the Event
III International Symposium on Fungal Stress – ISFUS
City of the Event
São José dos Campos
Title of the Proceedings of the event
Annals of the International Symposium on Fungal Stress – ISFUS
Name of the Publisher
Even3
Means of Dissemination
Meio Digital

How to cite

RIBEIRO, Felipe de Camargo et al.. APPLICATION OF PROBIOTICS FORMULATIONS TO PREVENT ORAL CANDIDIASIS: IN VITRO AND IN VIVO STUDY.. In: Annals of the International Symposium on Fungal Stress – ISFUS. Anais...São José dos Campos(SP) Hotel Nacional Inn, 2019. Available in: https//www.even3.com.br/anais/ISFUS/159431-APPLICATION-OF-PROBIOTICS-FORMULATIONS-TO-PREVENT-ORAL-CANDIDIASIS--IN-VITRO-AND-IN-VIVO-STUDY. Access in: 15/12/2025

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