PREVENTIVE AND THERAPEUTIC VASCULAR PHOTOBIOMODULATION (VPBM) ON ACUTE MUSCLE INJURY: EFFECTS ON VASCULAR ASPECTS DURING THE MUSCLE REPAIR IN AN ANIMAL MODEL

Aline Souza Silva; Tainá Caroline dos Santos Malavazzi; Maria Fernanda Setubal Destro Rodrigues; Sandra Kalil; Kristianne Porta Santos Fernandes; Fabio D. Nunes; Raquel Agnelli Mesquita-Ferrari

Título del Trabajo

Autores

Aline Souza Silva
Tainá Caroline dos Santos Malavazzi
Maria Fernanda Setubal Destro Rodrigues
Sandra Kalil
Kristianne Porta Santos Fernandes
Fabio D. Nunes
Raquel Agnelli Mesquita-Ferrari

Modalidad

Investigación

Área temática

Fisioterapia

Fecha de publicación

23/09/2024

País de publicación

Argentina | Argentina

Idioma de la Publicación

Inglês

Página del Trabajo

https://www.even3.com.br/anais/i-encuentro-latinoamericano-de-investigacion-y-practica-en-biofotonica-465848/893019-preventive-and-therapeutic-vascular-photobiomodulation-(vpbm)-on-acute-muscle-injury--effects-on-vascular-aspects

ISBN

978-65-272-0672-9

Palabras Clave

vascular photobiomodulation, cryoinjury, muscle repair

Resumen

Muscle injuries are common, and local photobiomodulation therapy (PBM) has been shown to be beneficial for repair. PBM has demonstrated positive effects on the regeneration of muscle tissue when applied before or after injury. However, vascular photobiomodulation (VPBM) has shown promising effects on tissue repair and has therefore been investigated . To evaluate the effects of vascular photobiomodulation (VPBM) applied preventively or therapeutically on acute muscle injury induced in Wistar rats, with a focus on the vascular aspects of tissue remodeling. Wistar rats (n = 85) were evaluated into five experimental groups (control, injury, non-injury+VPBM, Prior VPBM + injury and injury + post VPBM). Animals were transcutaneously irradiated over the tail artery/vein using a low-level AlGaAs diode laser (780 nm, 40 mW, 0.04 cm2, 3.2 J, 80 s) and applied prior to and after cryoinjury induction on the anterior tibialis muscle. Muscle samples were collected 1, 2, 5 and 7 days after the injury, processed, subjected to histological hematoxylin and eosin (HE) staining and evaluated by light microscopy to evaluate blood vessels. To evaluate hemoglobin and erythrocytes, blood samples were collected via intracardiac puncture and analyzed using an automatic quantitative hematological analyzer. An increase in erythrocyte count and hemoglobin concentration was observed in the Non-Injury + VPBM group on day 1 compared to the Injury and Control groups. The Prior VPBM + Injury and Injury + VPBM Post groups exhibited an increase in the presence of blood vessels after 1, 2, and 7 days compared to the Injury group. Additionally, on day 7, all evaluated groups showed increased vascularization, with the greatest intensity observed in the Prior VPBM + Injury group. VPBM treatment effectively modulated erythrocyte and hemoglobin levels and enhanced tissue vascularization at different experimental times, particularly when VPBM was applied prior to muscle injury.

Título del Evento: I ENCUENTRO LATINOAMERICANO DE INVESTIGACIÓN Y PRÁCTICA EN BIOFOTÓNICA.
Ciudad del Evento: Buenos Aires
Título de las Actas del Evento: Anais do Encuentro Latinoamericano de Investigación Y Práctica En Biofotónica.
Nombre de la Editora: Even3
Medio de Divulgación: Meio Digital

Como citar

SILVA, Aline Souza et al.. PREVENTIVE AND THERAPEUTIC VASCULAR PHOTOBIOMODULATION (VPBM) ON ACUTE MUSCLE INJURY: EFFECTS ON VASCULAR ASPECTS DURING THE MUSCLE REPAIR IN AN ANIMAL MODEL.. In: Anais do Encuentro Latinoamericano de Investigación Y Práctica En Biofotónica.. Anais...Sao Paulo(SP) UCU, 2024. Disponible en: https//www.even3.com.br/anais/i-encuentro-latinoamericano-de-investigacion-y-practica-en-biofotonica-465848/893019-PREVENTIVE-AND-THERAPEUTIC-VASCULAR-PHOTOBIOMODULATION-(VPBM)-ON-ACUTE-MUSCLE-INJURY--EFFECTS-ON-VASCULAR-ASPECTS. Acceso en: 16/07/2025