EFFECTS OF AN IMPROVED TOLUIDINE BLUE O PHOTOSENSITIZER FOR ANTIMICROBIAL PHOTODYNAMIC THERAPY ON GROWTH AND VIABILITY OF THE FIBROBLASTS L929

CLAUDIO TERUO KASSA; Aline Souza Silva; Valherya Silva Rodriguez; Raquel Agnelli Mesquita-Ferrari; Mark Wainwright; Christiane Pavani; Renato Prates

Título del Trabajo

Autores

CLAUDIO TERUO KASSA
Aline Souza Silva
Valherya Silva Rodriguez
Raquel Agnelli Mesquita-Ferrari
Mark Wainwright
Christiane Pavani
Renato Prates

Modalidad

Protocolos de investigación

Área temática

Odontología

Fecha de publicación

23/09/2024

País de publicación

Uruguai | Uruguay

Idioma de la Publicación

Inglês

Página del Trabajo

https://www.even3.com.br/anais/i-encuentro-latinoamericano-de-investigacion-y-practica-en-biofotonica-465848/892469-effects-of-an-improved-toluidine-blue-o-photosensitizer-for-antimicrobial-photodynamic-therapy-on-growth-and-viab

ISBN

978-65-272-0672-9

Palabras Clave

antimicrobial photodynamic therapy, photosensitizer, cytotoxicity

Resumen

INTRODUCTION The phenothiazinium derivative toluidine blue O is widely employed as a photosensitizer in Dentistry clinical trials. However, its activity against the pathogenic microorganisms is not significantly different to the most used methylene blue. This work will provide more information about the improved toluidine blue as known as butyl toluidine blue O – BuTBO, which was demonstrated to be better than others photosensitizers: increased in visible absorption wavelength and lipophilicity; decreased aggregation; higher production of singlet oxygen; remarkable increases in activity against Gram-negative Pseudomonas aeruginosa; and greater activity against conventional antibacterial and antifungal drugs as well as biocides commonly used for local disinfection.. OBJECTIVES Evaluate the cellular viability and growth in presence of methylene blue, toluidine blue O and butyl toluidine blue O in dark. Compare the effects among the three photosensitizers. Determine which concentration induce minimal cellular alterations. METHODOLOGY Cell culture will be cultured in Dulbecco´s modified Eagle´s medium supplement with 10% fetal bovine serum and antibiotics and antimycotics at 37°C in a humidified atmosphere of 5% CO2. They will be enzymatically dissociated with trypsin and will be counted under Neubauer chamber using trypan blue staining protocol and seeded in a 96-well plate, afterwards they will be stained for 1, 24 and 48h. Methylene blue, toluidine blue O and butyl toluidine blue O will be diluted on purified water at 100µM final concentration (approx. 0,003 mg/mL each) and will be serial diluted at 1:2 – started at 100µM and finish at 0,78µM. Afterwards, the cells will be assessed by MTT assay, Crystal violet assay and Flow cytometry. RESULTS Up to now, data have demonstrated no differences between photosensitizers in cellular growth and viability. CONCLUSION The photosensitizers used in this work had the same effects on the tested cells.

Título del Evento: I ENCUENTRO LATINOAMERICANO DE INVESTIGACIÓN Y PRÁCTICA EN BIOFOTÓNICA.
Ciudad del Evento: Montevideo
Título de las Actas del Evento: Anais do Encuentro Latinoamericano de Investigación Y Práctica En Biofotónica.
Nombre de la Editora: Even3
Medio de Divulgación: Meio Digital

Como citar

KASSA, CLAUDIO TERUO et al.. EFFECTS OF AN IMPROVED TOLUIDINE BLUE O PHOTOSENSITIZER FOR ANTIMICROBIAL PHOTODYNAMIC THERAPY ON GROWTH AND VIABILITY OF THE FIBROBLASTS L929.. In: Anais do Encuentro Latinoamericano de Investigación Y Práctica En Biofotónica.. Anais...Sao Paulo(SP) UCU, 2024. Disponible en: https//www.even3.com.br/anais/i-encuentro-latinoamericano-de-investigacion-y-practica-en-biofotonica-465848/892469-EFFECTS-OF-AN-IMPROVED-TOLUIDINE-BLUE-O-PHOTOSENSITIZER-FOR-ANTIMICROBIAL-PHOTODYNAMIC-THERAPY-ON-GROWTH-AND-VIAB. Acceso en: 02/05/2025