A NEW MONOPARTITE BEGOMOVIRUS CAUSING SEVERE LEAF DISTORTION AND MOTTLING IN TOMATO IN BRAZIL

Thais pereira martins; Dr. Tadeu Araújo de Souza; Erich Yukio Tempel Nakasu; Tatsuya Nagata; Alice Kazuko Inoue Nagata

All Papers

Submission

DOI

10.29327/cbv.297740

Paper Title

A NEW MONOPARTITE BEGOMOVIRUS CAUSING SEVERE LEAF DISTORTION AND MOTTLING IN TOMATO IN BRAZIL

Authors

Thais pereira martins
Dr. Tadeu Araújo de Souza
Erich Yukio Tempel Nakasu
Tatsuya Nagata
Alice Kazuko Inoue Nagata

Modality

Resumo

Subject area

Vegetal e Invertebrados

Publishing Date

04/01/2021

Country of Publishing

Brasil

Language of Publishing

Inglês

Paper Page

https://www.even3.com.br/anais/cbv/297740-a-new-monopartite-begomovirus-causing-severe-leaf-distortion-and-mottling-in-tomato-in-brazil

ISBN

978-65-5941-175-7

Keywords

tomato, begomovirus, Geminiviridae

Summary

Viruses of the genus Begomovirus have emerged as important plant pathogens in tropical and subtropical regions worldwide. It is the largest genus in the family Geminiviridae, with more than 400 described species. Most begomoviruses present in Brazil have a bipartite genome composed of DNA-A and DNA-B components. However, some viruses with a monopartite genome were described, such as tomato mottle leaf curl virus (ToMoLCV) and tomato leaf curl purple vein virus (ToLCPVV). Tomato plants are infected by many begomoviruses, more than any other crop, likely attributed to the high population of whiteflies (its vector), the wide range of hosts, the high susceptibility of tomatoes, and the high genetic variability of these viruses. During a survey on viral diseases, on April 2020, a tomato plant (cv. AP533) with strong symptoms of leaf distortion, chlorotic spots, vein clearing, mosaic and mottling was observed in a processing tomato field in Luziânia, Goiás state Brazil. The sample was named #22682. Therefore, aiming to identify the virus that was causing these symptoms, total DNA was extracted, the viral circular DNA was amplified by rolling-circle amplification (RCA), and sequenced. The obtained sequence shared <91% nucleotide identity with other known begomoviruses. Thus, a dimeric form of the genome was cloned in a binary vector to produce an infectious clone. The DNA-A-like sequence was 2.596 nucleotides-long. The BLASTn analysis of the genome showed that tomato golden leaf distortion virus (ToGLDV, HM357456) is the closest begomovirus, sharing 83% of nucleotide identity with 90% of query cover (E-value 0). The genome encodes one protein in the virion-sense strand, the coat protein (CP), and four proteins in the complementary-sense: replication-associated protein (Rep), transcriptional activator protein (TrAP), replication enhancer protein (Ren) and C4. In a phylogenetic analysis, the #22682 genome was grouped with ToGLDV (monopartite), pepper blistering leaf virus (bipartite), and tomato vein clearing leaf virus (bipartite). Agroinoculation of the dimeric clone resulted in the infection of tomato and tobacco plants, confirming the infectivity. These results suggest the monopartite nature of the virus. Based on the genome characteristics, and on the current ICTV species demarcation criteria, we propose that sample #22682 contained a putative new begomovirus species. The complete molecular and biological characterization of this virus is being conducted.

Title of the Event: XXXI Congresso Brasileiro de Virologia & XV Encontro de Virologia do Mercosul
Title of the Proceedings of the event: Anais do Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul
Name of the Publisher: Even3
Means of Dissemination: Meio Digital
DOI

How to cite

MARTINS, Thais pereira et al.. A NEW MONOPARTITE BEGOMOVIRUS CAUSING SEVERE LEAF DISTORTION AND MOTTLING IN TOMATO IN BRAZIL.. In: Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul. Anais...Porto Alegre(RS) Online, 2020. Available in: https//www.even3.com.br/anais/cbv/297740-A-NEW-MONOPARTITE-BEGOMOVIRUS-CAUSING-SEVERE-LEAF-DISTORTION-AND-MOTTLING-IN-TOMATO-IN-BRAZIL. Access in: 12/05/2025