Anais do Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul

MOLECULAR DETECTION OF FLAVIVIRUS AND ALPHAVIRUS AS A DIAGNOSTIC TOOL FOR ARBOVIRUSES SURVEILLANCE IN AN ENDEMIC AREA

Published in 04/01/2021 - ISBN: 978-65-5941-175-7

Link All Papers
LinkSubmission
DOI
10.29327/cbv.297577  
Paper Title
MOLECULAR DETECTION OF FLAVIVIRUS AND ALPHAVIRUS AS A DIAGNOSTIC TOOL FOR ARBOVIRUSES SURVEILLANCE IN AN ENDEMIC AREA
Authors
  • Izabela Batista Melo
  • SULEIMY MARINHO FERNANDES
  • Esther Ferreira Leão Neto
  • Valéria Christina de Rezende Féres
  • MENIRA BORGES DE LIMA DIAS E SOUZA
  • Fernanda Craveiro Franco
  • Fabíola Fiaccadori
Modality
Resumo
Subject area
Humana
Publishing Date
04/01/2021
Country of Publishing
Brasil
Language of Publishing
Inglês
Paper Page
https://www.even3.com.br/anais/cbv/297577-molecular-detection-of-flavivirus-and-alphavirus-as-a-diagnostic-tool-for-arboviruses-surveillance-in-an-endemic-
ISBN
978-65-5941-175-7
Keywords
Arboviruses, Flavivirus, Alphavirus, molecular, surveillance
Summary
Arboviruses (or arthropod-borne viruses) are known for their mode of transmission through the bite of blood-sucking arthropod vectors. Flavivirus and Alphavirus are the genera comprising the most relevant arboviruses related to disease in humans, with particular emphasis for the Flavivirus dengue (DENV), yellow fever (YFV) and zika (ZIKV), and the Alphavirus chikungunya (CHIKV) and mayaro (MAYV) in Brazil. The Midwest region of Brazil is considered endemic for these viruses. The state of Goiás, especially the city of Goiânia, is an area constantly affected by DENV, presenting with widely populated urban centers infested by main vector, Aedes aegypti. Recently, regional studies have described the occurrence of febrile patients infected with MAYV in Goiânia and nearby rural areas. Also, the co-circulation of ZIKV and CHIKV since 2016 has been of great concern. In this context, a molecular investigation for Flavivirus and Alphavirus was carried out in Goiânia. Serum samples were collected from patients with clinical suspicion for arboviruses at different public health facilities between April and June of 2018, and April and October of 2019. Viral RNA extraction was performed using a commercial kit, according to the manufacturer’s instructions. Molecular investigation by RT-qPCR for Flavivirus and RT-qPCR for Alphavirus was performed. Positive samples were subsequently submitted to specific protocols for DENV, ZIKV, YFV, CHIKV and MAYV. DENV positive samples were additionally submitted to a RT-PCR protocol for serotyping. Positivity was detected only for DENV. In 2018, from a total of 60 samples, a general positivity index of 26.7% was observed, being that 50.0% were female patients, 37.5% included in the age group of 40 to 49 years and 68.8% from patients with 1 to 3 days of symptoms. As for 2019, a total of 48 samples were included. A positivity of 16.7% was observed, 62.5% for female patients, 50.0% included in the age group greater than or equal to 50 years and 87.5% collected when the patients presented with 4 to 6 days of symptoms. DENV-2 was the only serotype identified. Epidemiological data provided by the Municipal Health Department indicates DENV-2 as the main serotype in circulation during the same sample period, followed by serotype 1 and 4. The identification of circulating serotypes and strains is crucial for elucidation of dispersion and virulence patterns of disease, thereby, the detection of viral RNA provides a quick and sensitive diagnosis, highlighting the importance of using molecular techniques for better monitoring and surveillance of arboviruses. In this sense, we established a protocol for molecular detection of Flavivirus and Alphavirus, contributing for the surveillance system, in a way that can accurately define and diagnose infections by arboviruses that are classified in such genera and considered the most abundant in this endemic region. Financial Support: CAPES and FAPEG
Title of the Event
XXXI Congresso Brasileiro de Virologia & XV Encontro de Virologia do Mercosul
Title of the Proceedings of the event
Anais do Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul
Name of the Publisher
Even3
Means of Dissemination
Meio Digital
DOI

How to cite

MELO, Izabela Batista et al.. MOLECULAR DETECTION OF FLAVIVIRUS AND ALPHAVIRUS AS A DIAGNOSTIC TOOL FOR ARBOVIRUSES SURVEILLANCE IN AN ENDEMIC AREA.. In: Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul. Anais...Porto Alegre(RS) Online, 2020. Available in: https//www.even3.com.br/anais/cbv/297577-MOLECULAR-DETECTION-OF-FLAVIVIRUS-AND-ALPHAVIRUS-AS-A-DIAGNOSTIC-TOOL-FOR-ARBOVIRUSES-SURVEILLANCE-IN-AN-ENDEMIC-. Access in: 29/05/2025

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