Anais do Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul

SURVEY ON MYCOVIRUSES OF THE SOIL PATHOGENS: SCLEROTINIA SCLEROTIORUM AND VERTICILLIUM DAHLIAE

Published in 04/01/2021 - ISBN: 978-65-5941-175-7

Link All Papers
LinkSubmission
DOI
10.29327/cbv.297136  
Paper Title
SURVEY ON MYCOVIRUSES OF THE SOIL PATHOGENS: SCLEROTINIA SCLEROTIORUM AND VERTICILLIUM DAHLIAE
Authors
  • Alba Suaste Dzul
  • Erich Yukio Tempel Nakasu
  • VALDIR LOURENÇO JUNIOR
  • Eduardo Seiti Gomide Mizubuti
  • Alice Kazuko Inoue Nagata
  • AILTON REIS
Modality
Resumo
Subject area
Vegetal e Invertebrados
Publishing Date
04/01/2021
Country of Publishing
Brasil
Language of Publishing
Inglês
Paper Page
https://www.even3.com.br/anais/cbv/297136-survey-on-mycoviruses-of-the-soil-pathogens--sclerotinia-sclerotiorum-and-verticillium-dahliae
ISBN
978-65-5941-175-7
Keywords
RNA-seq, mycovirus, soil-borne pathogens
Summary
Mycoviruses are viruses that can infect and replicate in fungi, including plant pathogenic fungi. Their hypovirulent property has attracted attention because of its potential use in the biological control strategy. Sclerotinia sclerotiorum and Verticillium dahliae are two notorious soil-borne pathogens with a broad host range, being both of them difficult to control by conventional chemical application. In this study, we used a metagenomics approach to discover mycoviruses that could induce a hypovirulence infection on these fungi. We selected 44 and 42 field-collected isolates of V. dahliae and S. sclerotiorum, respectively. Total RNA was extracted from mycelia of each isolate, pooled, and prepared for high-throughput sequencing after Ribo-Zero rRNA H/M/R treatment. cDNA libraries were constructed using TruSeq Stranded Total RNA sample preparation kit (Illumina) and sequenced on an Illumina NovaSeq 6000 platform. Paired-end reads with lengths of 100 nucleotides (nt) were used for de novo assembly with the Trinity software. The obtained contigs were analyzed by the BLASTn tool to detect sequences homologous to previously described mycoviruses. From the S. sclerotiorum sample pool, we identified three potential mycovirus contigs: (1) the first of 3,461 nt shares 84.0 % nt identity with Sclerotinia sclerotinia ourmia-like virus and consists of a putative ORF which encodes a protein of 688 amino acid (aa) of an RNA-dependent RNA polymerase (RdRp)-like protein; (2) the second of 3,427 nt shares 96.4 % nt identity with Sclerotinia sclerotiorum mitovirus 6, and the putative ORF encodes a protein of 584 aa homologous to an RdRp; and (3) the third of 9,656 nt sharing 97.0 % identity with Sclerotinia sclerotiorum negative stranded RNA virus, which encodes an RdRp-like protein of 2010 aa. From the V. dahliae pooled sample, we detected three contigs similar to an unclassified RNA virus. One of these sequences shares 74.9 % nt identity to a soil mycovirus sequence. Our results show that mycoviruses are present in S. sclerotiorum and V. dahliae isolates and are potential candidates as biocontrol agents of these plant pathogens. Financial support: This research was financially supported by FAP-DF (Processo: 0193.001197/2016), Embrapa Hortaliças, and CAPES.
Title of the Event
XXXI Congresso Brasileiro de Virologia & XV Encontro de Virologia do Mercosul
Title of the Proceedings of the event
Anais do Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul
Name of the Publisher
Even3
Means of Dissemination
Meio Digital
DOI

How to cite

DZUL, Alba Suaste et al.. SURVEY ON MYCOVIRUSES OF THE SOIL PATHOGENS: SCLEROTINIA SCLEROTIORUM AND VERTICILLIUM DAHLIAE.. In: Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul. Anais...Porto Alegre(RS) Online, 2020. Available in: https//www.even3.com.br/anais/cbv/297136-SURVEY-ON-MYCOVIRUSES-OF-THE-SOIL-PATHOGENS--SCLEROTINIA-SCLEROTIORUM-AND-VERTICILLIUM-DAHLIAE. Access in: 05/08/2025

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