Anais do Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul

BRAZILIAN COMMERCIAL VACCINES AGAINST CPV-2 GENETICALLY DIFFER FROM FIELD STRAINS IN THE ANTIGENIC PROTEIN VP2

Published in 04/01/2021 - ISBN: 978-65-5941-175-7

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LinkSubmission
DOI
10.29327/cbv.297037  
Paper Title
BRAZILIAN COMMERCIAL VACCINES AGAINST CPV-2 GENETICALLY DIFFER FROM FIELD STRAINS IN THE ANTIGENIC PROTEIN VP2
Authors
  • Laura Morais Nascimento Silva
  • Marcus Rebouças Santos
  • Viviane Sisdelli Assao
  • Jordano Alexandre Carvalho
  • Larissa Lana de Paula Leber
  • Nívia Carolina Lopes Rosado
  • Lidiany Lopes Gomes
  • Junio Cesar Santos
  • Ana Alice Pimenta Pereira
  • Silvia de Oliveira Hubner
  • Abelardo Silva Júnior
Modality
Resumo
Subject area
Veterinária
Publishing Date
04/01/2021
Country of Publishing
Brasil
Language of Publishing
Inglês
Paper Page
https://www.even3.com.br/anais/cbv/297037-brazilian-commercial-vaccines-against-cpv-2-genetically-differ-from-field-strains-in-the-antigenic-protein-vp2
ISBN
978-65-5941-175-7
Keywords
Carnivore protoparvovirus 1, genetic discrepancy, non-syninimous mutations.
Summary
Carnivore protoparvovirus 1 (CPPV-1) causes enteritis in carnivores worldwide. Canine parvovirus 2 (CPV-2) is a strain belonging to the CPPV-1. CPV-2 strain is frequent in dogs and eventually found in cats. CPV-2 is divided into CPV-2a, CPV-2b, and CPV-2c variants, which were established due to mutations in the protein VP2. Such mutations are related to the viral evade from the immune system. Accordingly, this study aimed to evaluate mutations of amino acid residues of CPV-2 VP2 protein obtained from domestic carnivores presenting enteritis and from vaccine strains. Sixteen feces samples of dogs and one blood sample of a cat (S1 to S17) were obtained in Zona da Mata and Belo Horizonte Metropolitan region, State of Minas Gerais. Seven commercial vaccines against CPV-2 were further analyzed (Vac1 to Vac7). All field strains were isolated in CRFK cell culture. The PCR assay amplified the C-terminus VP2 region of 583bp, which characterizes CPPV-1 variants. Amino acid sequences were aligned and submitted to phylogenetic analysis with other CPPV-1 sequences from other Brazilian regions and countries. Sixteen CPV-2 variants obtained in this study were CPV-2b (Asn426Asp) (16/17) and one was CPV-2-like (Asn426 and Ile555Val) (1/17). Commercial vaccines Vac1 to Vac5 (5/7) presented a not current strain CPV-2 (Asn426 and Val555), isolated in 1978. Vac3 presented an additional substitution Tyr573Phe. Vac6 strain was CPV-2a (Asn426 and Val555Ile) and Vac7 strain was CPV2-b (Asn426Asp and Val555). Notably, Vac7 showed other three amino acid residue substitutions: the non-synonymous Asp413Asn and Lys570Glu, and the synonymous Lys575Arg. Sample S2, characterized as CPV-2b, was obtained from a puppy that had a complete vaccination record with the vaccine Vac2 (CPV-2 not current strain). By comparing the phylogenetic relationship between the samples obtained in this study and other CPPV-1 strains and CPV-2 variants from different countries, wild parvoviruses clustered in two different monophyletic clades in the phylogenetic tree. All CPV-2b variants grouped in the same clade, however, were found not to cluster with other Brazilian isolates. The wild variant CPV-2-like presented higher phylogenetic similarity with CPV-2 from Vac4 and with a CPV-2a from China. Furthermore, this variant had phylogenetic similarity with all the vaccine strains CPV-2 and CPV-2a. Importantly, CPVs isolated in this study clustered distant from the commercial vaccine strains available to the immunization of the Brazilian canine population. The vaccine strain CPV-2b from Vac7 was the single vaccine presenting a higher relationship with CPV-2b variants obtained in this study. This study represents the first attempt to the molecular characterization of CPV-2 variants causing gastroenteritis domestic carnivores from the State of Minas Gerais, Brazil. Five strains from commercial vaccines appeared to be distantly phylogenetically related to CPV-2 Brazilian strains. Additionally, due to synonymous and non-synonymous mutations of amino acid residues in the antigenic protein VP2, two commercial vaccines presented remarkable dissimilarities compared to the wild variants obtained in this study. The genetic diversity among CPV-2 field strains should be a concern of some vaccine companies, and novel studies should be conducted to explore new vaccine candidates. Financial support: FAPEMIG, CAPES and CNPq
Title of the Event
XXXI Congresso Brasileiro de Virologia & XV Encontro de Virologia do Mercosul
Title of the Proceedings of the event
Anais do Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul
Name of the Publisher
Even3
Means of Dissemination
Meio Digital
DOI

How to cite

SILVA, Laura Morais Nascimento et al.. BRAZILIAN COMMERCIAL VACCINES AGAINST CPV-2 GENETICALLY DIFFER FROM FIELD STRAINS IN THE ANTIGENIC PROTEIN VP2.. In: Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul. Anais...Porto Alegre(RS) Online, 2020. Available in: https//www.even3.com.br/anais/cbv/297037-BRAZILIAN-COMMERCIAL-VACCINES-AGAINST-CPV-2-GENETICALLY-DIFFER-FROM-FIELD-STRAINS-IN-THE-ANTIGENIC-PROTEIN-VP2. Access in: 09/05/2025

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