INCREASED LEVELS OF CIRCULATING HIGH MOBILITY GROUP BOX 1 (HMGB1) PROTEIN IN CHIKUNGUNYA VIRUS INFECTION

Daniele Cristina Passos da Rocha; Ronaldo Mohana-Borges; Marciano Viana Paes; Diego Allonso

All Papers

Submission

DOI

10.29327/cbv.291215

Paper Title

INCREASED LEVELS OF CIRCULATING HIGH MOBILITY GROUP BOX 1 (HMGB1) PROTEIN IN CHIKUNGUNYA VIRUS INFECTION

Authors

Daniele Cristina Passos da Rocha
Ronaldo Mohana-Borges
Marciano Viana Paes
Diego Allonso

Modality

Resumo

Subject area

Humana

Publishing Date

04/01/2021

Country of Publishing

Brasil

Language of Publishing

Inglês

Paper Page

https://www.even3.com.br/anais/cbv/291215-increased-levels-of-circulating-high-mobility-group-box-1-(hmgb1)-protein-in-chikungunya-virus-infection

ISBN

978-65-5941-175-7

Keywords

Chikv, HMGB1, arthralgia

Summary

Chikungunya virus (CHIKV) is the causative agent of one of the most debilitating disease, named Chikungunya fever. Its most common clinical manifestations are fever, body pains, and arthralgia, which may persist in some individuals several months or years after complete virus clearance. Although rarely fatal, CHIKV infection is an important social and economic burden worldwide. After the acute phase, which lasts around 15 days, at least 40% of patients progress to the chronic phase, with prolonged inflammatory disorders, with rheumatic and systemic symptoms. The High Mobility Group Box 1 (HMGB1) protein is a non-classical pro-inflammatory mediator usually overexpressed in rheumatoid arthritis. It can be secreted by activated monocytes in response to exogenous or endogenous inflammatory stimuli (such as how LPS, TNF-a, IL-1, and IFN-?) or be passively released by necrotic cells. HMGB1 is responsible for signaling immune cells to the site of inflammation as well as for tissue healing after damage, being characterized as a damage-associated molecular pattern (DAMP). In this study, we aim to identify whether HMGB1 was increased during the acute phase of CHIKV infection. To quantify HMGB1, it was used a plasma panel with samples from patients infected by CHIKV or co-infected with dengue and ZIKA, and plasma of healthy individuals were used as negative control. Circulating levels of HMGB1 was measured by a quantitative capture ELISA developed by our group. CHIKV, ZIKV and DENV infection were confirmed by qPCR and CHIKV viral load was calculated. HMGB1 levels were significantly higher in CHIKV-positive samples compared to the negative control, with an average serum concentration of 516.12 ng / mL (SEM ± 48.83 ng / mL) versus 132.33 ng / mL (SEM ± 69.81). The mean plasma concentration of HMGB1 for DENV4 / CHIKV was 605.93 ng / mL (SEM ± 121.46 ng / mL) and for ZIKV / CHIKV was 688.83 ng / mL (SEM ± 85.61 ng / mL). Samples collected during the disease's convalescence phase revealed a low plasma level of HMGB1, similar to the values observed in the negative control group (p = 0.3329, 63.18 ng /mL, SEM ± 26.27 ng / mL). These data show that HMGB1 levels are increased in patients infected with CHIKV and remain elevated throughout the acute phase of the disease but not during convalescent phase. The HMGB1 levels were plotted against viral charge and the result indicated that they are positively correlated, suggesting that the higher is viral charge, the higher is HMGB1 release to circulation. Overal these results show that HMGB1 is an important inflammatory mediator produced during CHIKV infection that can be involved in the severity of disease. Financial Support: CAPES, CNPq e FAPERJ

Title of the Event: XXXI Congresso Brasileiro de Virologia & XV Encontro de Virologia do Mercosul
Title of the Proceedings of the event: Anais do Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul
Name of the Publisher: Even3
Means of Dissemination: Meio Digital
DOI

How to cite

ROCHA, Daniele Cristina Passos da et al.. INCREASED LEVELS OF CIRCULATING HIGH MOBILITY GROUP BOX 1 (HMGB1) PROTEIN IN CHIKUNGUNYA VIRUS INFECTION.. In: Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul. Anais...Porto Alegre(RS) Online, 2020. Available in: https//www.even3.com.br/anais/cbv/291215-INCREASED-LEVELS-OF-CIRCULATING-HIGH-MOBILITY-GROUP-BOX-1-(HMGB1)-PROTEIN-IN-CHIKUNGUNYA-VIRUS-INFECTION. Access in: 04/05/2025