Anais do Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul

TERM PLACENTAL EXTRAVILLOUS CYTOTROPHOBLAST CELLS ARE INFECTED BY ZIKA VIRUS

Published in 04/01/2021 - ISBN: 978-65-5941-175-7

Link All Papers
LinkSubmission
DOI
10.29327/cbv.291192  
Paper Title
TERM PLACENTAL EXTRAVILLOUS CYTOTROPHOBLAST CELLS ARE INFECTED BY ZIKA VIRUS
Authors
  • Karen Steponavicius Cruz Borbely
  • Keyla Silva Nobre Pires
  • Aldilane Lays Xavier Marques
  • Maria Juliana Calado Ramalho Dos Santos
  • Eloiza Tanabe
  • Elaine Cristina Oliveira Silva
  • SAMUEL TEIXEIRA DE SOUZA
  • Desconhecido2127
  • Alexandre Urban Borbely
Modality
Resumo
Subject area
Humana
Publishing Date
04/01/2021
Country of Publishing
Brasil
Language of Publishing
Inglês
Paper Page
https://www.even3.com.br/anais/cbv/291192-term-placental-extravillous-cytotrophoblast-cells-are-infected-by-zika-virus
ISBN
978-65-5941-175-7
Keywords
PLACENTA, ZIKA VIRUS, TROPHOBLAST
Summary
Introduction: Zika virus (ZIKV) infection during late pregnancy was associated with infant immune-mediated neurological complications, which were fairly shadowed by the severe Congenital Zika virus Syndrome derived by first trimester infections. At term, although some studies were conducted using term chorionic villi explants, none whatsoever was conducted on extravillous cytotrohoblast (EVT) cells. These cells are crucial components of human placenta, participating since the corrrect placentation to a plethora of functions during the entire pregnancy that are responsible for local maintenance of the maternal-fetal interface. Therefore, we aimed to establish if the ZIKV is capable to infect term EVT cells, as well as to show expression differences on known viral receptors expression that could be linked to ZIKV entrance. Material and Methods: Term placentas were collected from the Obstetrics service from HUPAA/UFAL under informed consent, and EVT cells were isolated and cultured. Cell purity was accessed by HLA-G expression by flow cytometry. Cells were infected with 1 MOI of ZIKV strain PE243 for 24 h, and after media renewal, for further 24 h in culture. The supernatants viral load were detected by qRT-PCR, and cellular content of NS1 ZIKV proteins by immunofluorescence and flow cytometry. Phalloidin staining was also employed to show F-actin polymerization, and atomic force microscopy was employed for cell topography. Receptors FcRn, TLR-7 and TLR-8 expression, as well as p-STAT-2 expression were detected by flow cytometry. Results: Cell preparations had a purity of 75.25% HLA-G positive cells, and they showed 53.3% positivity for ZIKV NS1. Immunofluorescence confirmed ZIKV NS1 expression in EVT cell cytoplasm, and the supernatant viral load after 24 h was 0.545 x 105 PFU/µL. Phalloidin staining depicted reduced F-actin polymerization and loss of stress fiber. The AFM analysis showed smaller cells, with less prolongations, reduced cell height, increased rugosity and increased amount of macrovesicles release with the size of apoptotic bodies. The expression of p-STAT-2, FcRN and TLR-7 were unchanged, but TLR-8 expression was slightly reduced in the infected group. Conclusion: We effectively showed that EVT cells can be infected by ZIKV in late pregnancy, inducing morphological alterations that are suggestive of cell death. Nevertheless, more studies are needed to unveil which receptors are associated with viral entry, as well as the signaling induced by ZIKV infection in EVT cells. Financial support: Decit/MS/CNPq/SESAU-AL/FAPEAL n.06/2016 - PPSUS: 60030 000819/2016.
Title of the Event
XXXI Congresso Brasileiro de Virologia & XV Encontro de Virologia do Mercosul
Title of the Proceedings of the event
Anais do Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul
Name of the Publisher
Even3
Means of Dissemination
Meio Digital
DOI

How to cite

BORBELY, Karen Steponavicius Cruz et al.. TERM PLACENTAL EXTRAVILLOUS CYTOTROPHOBLAST CELLS ARE INFECTED BY ZIKA VIRUS.. In: Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul. Anais...Porto Alegre(RS) Online, 2020. Available in: https//www.even3.com.br/anais/cbv/291192-TERM-PLACENTAL-EXTRAVILLOUS-CYTOTROPHOBLAST-CELLS-ARE-INFECTED-BY-ZIKA-VIRUS. Access in: 28/06/2025

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