Anais do Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul

GENOMIC CHARACTERIZATION OF AN ALPHABACULOVIRUS INFECTIOUS TO THE COTTON ARMYWORM, ALABAMA ARGILLACEA

Published in 04/01/2021 - ISBN: 978-65-5941-175-7

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LinkSubmission
DOI
10.29327/cbv.290728  
Paper Title
GENOMIC CHARACTERIZATION OF AN ALPHABACULOVIRUS INFECTIOUS TO THE COTTON ARMYWORM, ALABAMA ARGILLACEA
Authors
  • Hugo de Paula Oliveira
  • Daniel Polita
  • MIGUEL DE SOUZA ANDRADE
  • Daniel Ricardo Sosa Gomez
  • Bergmann Morais Ribeiro
  • Daniel Ardisson-Araujo
Modality
Resumo
Subject area
Vegetal e Invertebrados
Publishing Date
04/01/2021
Country of Publishing
Brasil
Language of Publishing
Inglês
Paper Page
https://www.even3.com.br/anais/cbv/290728-genomic-characterization-of-an-alphabaculovirus-infectious-to-the-cotton-armyworm-alabama-argillacea
ISBN
978-65-5941-175-7
Keywords
Baculovirus, Alphabaculovirus, Alabama argillacea
Summary
Cotton crops (Gossypium spp.) provide the raw material for various uses and play an important role in the agricultural sphere, especially in Brazil. Therefore, losses in production significantly affect the country's economy. One of the most severe cotton pests in Neotropics is the cotton armyworm, Alabama argillacea (Hübner) (Lepidoptera: Noctuidae), which is a monophagous defoliator during larval stages. Unfortunately in a measure of pest control, Brazilian cotton culture has a history of intensive pesticide abuse. Thus, biological control strategies such as that based on the use of baculovirus may represent an alternative and safe strategy to protect cotton crops. However, no baculovirus was described so far infecting this insect. In this work, we describe the complete genome sequence of a novel baculovirus isolated from the cotton armyworm. The virus was obtained from dead subjects of A. argillacea with symptoms of nuclear polyhedrosis from the EMBRAPA-Soja virus collection. No information of place and date of collection was available. The sequencing was performed using the 454 pyrosequencing method. The virus was named by Alabama argillacea nucleopolyhedrovirus isolate CNPSo-94 (AlarNPV-CNPSo-94). Comparative genomic analyses were carried out in the software Geneious R9. A total of 24,745 initial reads were processed and used for de novo assembly with a Geneious algorithm. Genes were annotated with the same program and characterized in silico by the BLASTx tool. The Kimura-2 parameter-based criterion for baculovirus species demarcation was used with the partial sequence of some selected genes. The phylogeny was generated with the FasTree method based on the 38 baculovirus core genes. Single nucleotide polymorphisms (SNPs) counts were performed for each paralogous gene in related isolates. The relative position of each gene was identified and illustrated on a synteny map against other baculovirus genomes using the CIRCOS software. Moreover, the selection pressure for each gene was analyzed using the PAMLx software. We found that the AlarNPV-CNPSo-94 genome has 44.5% G+C content with a size of 129.743 bp. We annotated 152 ORFs and identified AlarNPV-CNPSo-94 as a member of species Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV), as a sister lineage of the more recent common ancestor shared with the isolates AgMNPV-30 and AgMNPV-38. Isolates 30 and 38 were found in Uruguay. In addition, there is a variability of SNPs among genes considering all related isolates. We found only 12 genes of the AlarNPV-CNPSo-94 genome under diversifying selection pressure when compared to the other isolates of the same species. Regarding genome structure, the AgMNPV-2D genome was found to be strictly collinear with AlarNPV-CNPSo-94 and some punctual translocations were found when compared to the CfDEFMNPV and CoveMNPV genomes. The AcMNPV-E2 genome presented an inversion, except for a collinear band at the end of the genome. Overall, the molecular characterization of the new A. argillacea infectious baculovirus and the unexpected discovery that this virus belongs to species AgMNPV gives us scope for further studies of agricultural interest since the here described NPV can infect the cotton armyworm, and possibly the soybean caterpillar, Anticarsia gemmatalis.
Title of the Event
XXXI Congresso Brasileiro de Virologia & XV Encontro de Virologia do Mercosul
Title of the Proceedings of the event
Anais do Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul
Name of the Publisher
Even3
Means of Dissemination
Meio Digital
DOI

How to cite

OLIVEIRA, Hugo de Paula et al.. GENOMIC CHARACTERIZATION OF AN ALPHABACULOVIRUS INFECTIOUS TO THE COTTON ARMYWORM, ALABAMA ARGILLACEA.. In: Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul. Anais...Porto Alegre(RS) Online, 2020. Available in: https//www.even3.com.br/anais/cbv/290728-GENOMIC-CHARACTERIZATION-OF-AN-ALPHABACULOVIRUS-INFECTIOUS-TO-THE-COTTON-ARMYWORM-ALABAMA-ARGILLACEA. Access in: 08/05/2025

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