DEVELOPMENT OF A BIOASSAY TO SCREENING ANTIVIRAL CANDIDATES COMPOUNDS FOR ZIKA VIRUS INHIBITION

Rhubia Silveira Martins Rosa; Desconhecido4718

All Papers

Submission

DOI

10.29327/cbv.285279

Paper Title

DEVELOPMENT OF A BIOASSAY TO SCREENING ANTIVIRAL CANDIDATES COMPOUNDS FOR ZIKA VIRUS INHIBITION

Authors

Rhubia Silveira Martins Rosa
Desconhecido4718

Modality

Resumo

Subject area

Básica

Publishing Date

04/01/2021

Country of Publishing

Brasil

Language of Publishing

Inglês

Paper Page

https://www.even3.com.br/anais/cbv/285279-development-of-a-bioassay-to-screening-antiviral-candidates-compounds-for-zika-virus-inhibition

ISBN

978-65-5941-175-7

Keywords

biosensor, bioassay, cytoprotection, cytotoxicity, screening, flavivirus

Summary

The Zika virus is an arbovirus that has triggered a worldwide epidemiology concern in the past years due to development of severe clinical conditions, especially in newborns of women infected during pregnancy. These newborns have shown the development of neuropathies such as microcephaly, brain calcifications, ophthalmological, hearing and motor problems, grouped in the so-called congenital Zika virus syndrome. The spread of the virus in more than 80 countries during the 2015 epidemic has attracted attention to this viral species and raised the urge of development of therapeutics strategies to fight this infection. In order to develop a new strategy capable of evaluating compounds that had antiviral activity and cytoprotective effect using a faster and more dynamic model, we established a bioassay based on the use of reporter cells. These reporter cells are genetically modified for cytoplasmic expression of GFP. The cytoplasmic GFP works as a biosensor for viral infection. The infection progression is associated to a decrease in the reporter's signal that may be monitored by flow cytometry. The antiviral or cytoprotective compounds may rescue the GFP biosensor signal. Using the GFP-biosensor based assay, we could evaluate the antiviral potential of candidate compounds. The biosensor results were similar to gold-standard tests as plaque forming assay and LDH quantification. The GFP bioassay may be adapted to a high-throughput screening enabling the evaluation of compound collections for the development of drugs, to prevent and treat Zika virus infections, as well to be used for other emerging viruses that a little is known about its biology and pathogenic mechanisms. Financial Support: We thank National Council for Scientific and Technological Development (CNPq), grant 440379/2016-4, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), grant 88881 – 130767/2016-01. We also thank Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for PhD fellowship (RSMR).

Title of the Event: XXXI Congresso Brasileiro de Virologia & XV Encontro de Virologia do Mercosul
Title of the Proceedings of the event: Anais do Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul
Name of the Publisher: Even3
Means of Dissemination: Meio Digital
DOI

How to cite

ROSA, Rhubia Silveira Martins; , Desconhecido4718. DEVELOPMENT OF A BIOASSAY TO SCREENING ANTIVIRAL CANDIDATES COMPOUNDS FOR ZIKA VIRUS INHIBITION.. In: Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul. Anais...Porto Alegre(RS) Online, 2020. Available in: https//www.even3.com.br/anais/cbv/285279-DEVELOPMENT-OF-A-BIOASSAY-TO-SCREENING-ANTIVIRAL-CANDIDATES-COMPOUNDS-FOR-ZIKA-VIRUS-INHIBITION. Access in: 10/05/2025