MOBILOME ANALYSIS OF A NEW BRAZILIAN MIMIVIRUS ISOLATE REVEALS A PROVIROPHAGE FULLY INTEGRATED IN ITS GENOME.

Bruna Luiza de Azevedo; Rodrigo Araújo Lima Rodrigues; Erna Geessien Kroon; Jônatas Santos Abrahão

All Papers

Submission

DOI

10.29327/cbv.282462

Paper Title

MOBILOME ANALYSIS OF A NEW BRAZILIAN MIMIVIRUS ISOLATE REVEALS A PROVIROPHAGE FULLY INTEGRATED IN ITS GENOME.

Authors

Bruna Luiza de Azevedo
Rodrigo Araújo Lima Rodrigues
Erna Geessien Kroon
Jônatas Santos Abrahão

Modality

Resumo

Subject area

Básica

Publishing Date

04/01/2021

Country of Publishing

Brasil

Language of Publishing

Inglês

Paper Page

https://www.even3.com.br/anais/cbv/282462-mobilome-analysis-of-a-new-brazilian-mimivirus-isolate-reveals-a-provirophage-fully-integrated-in-its-genome

ISBN

978-65-5941-175-7

Keywords

mimivirus, virophage, mobilome, transpoviron, provirophage

Summary

The discovery of mimiviruses raised interest for researches involving biological and molecular characterization of these giant viruses. It was proposed that mimiviruses have its own mobilome, a set of mobile genetic elements containing transpovirons and provirophages. Transpovirons are linear DNA sequences that encode proteins homologous to those of virophages and giant viruses. They can move within the same genome or between different genomes which makes them important to horizontal gene transfer and thus evolution. Virophages are smaller viruses that require, besides the amoeba host, a coinfection with a giant virus to establish its progeny. Previous studies described virophages putatively integrated in mimiviruses genomes, which were named provirophages. There are evidences suggesting a small common nucleotide region present in both viruses’ genes that encode for collagen-like repeat-containing proteins as responsible for giant virus-virophage interaction. The aim of this work was to perform genomic and phylogenetic characterization of a new mimivirus isolated by our group, which is associated to a transpoviron and a provirophage. Such characterization was made through viral genome sequencing using Illumina MiSeq platform and through functional annotation of its predicted genes by BLASTp and InterProScan analysis. Phylogenetic trees were reconstructed with the maximum likelihood method using the IQTree software. The analysis allowed the identification of the new isolate as a lineage A mimivirus, having a 1.2 Mb genome. The presence of a transpoviron sequence (6.624 bp and 6 genes) was confirmed, as well as a virophage (18.415 bp and 20 genes) integrated into the giant virus genome. The detected transpoviron is phylogenetically related to other previously described transpovirons found in lineage A mimiviruses. The isolated virophage is phylogenetically related to isolates of the Sputnik group. To our knowledge this is the first description of fully integration of a virophage sequence in a mimivirus genome. Analyzing the mimivirus genes that compose the integration region neighborhood we observed the presence of collagen-like repeat-containing genes both in the end and the start of the insertion, which suggests that these proteins may be important to the relationship between the virophage and its viral host. Thus, the genomic characterization performed in this work increases the knowledge about the mobilome of mimiviruses. Financial support: CNPq, CAPES, FAPEMIG, PRPq-UFMG, Ministério da Saúde-Decit.

Title of the Event: XXXI Congresso Brasileiro de Virologia & XV Encontro de Virologia do Mercosul
Title of the Proceedings of the event: Anais do Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul
Name of the Publisher: Even3
Means of Dissemination: Meio Digital
DOI

How to cite

AZEVEDO, Bruna Luiza de et al.. MOBILOME ANALYSIS OF A NEW BRAZILIAN MIMIVIRUS ISOLATE REVEALS A PROVIROPHAGE FULLY INTEGRATED IN ITS GENOME... In: Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul. Anais...Porto Alegre(RS) Online, 2020. Available in: https//www.even3.com.br/anais/cbv/282462-MOBILOME-ANALYSIS-OF-A-NEW-BRAZILIAN-MIMIVIRUS-ISOLATE-REVEALS-A-PROVIROPHAGE-FULLY-INTEGRATED-IN-ITS-GENOME. Access in: 04/06/2025