Anais do Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul

ANALYSIS OF A MARSEILLEVIRUS TRANSCRIPTOME REVEALS TEMPORAL GENE EXPRESSION PROFILE AND HOST TRANSCRIPTIONAL SHIFT

Published in 04/01/2021 - ISBN: 978-65-5941-175-7

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LinkSubmission
DOI
10.29327/cbv.282152  
Paper Title
ANALYSIS OF A MARSEILLEVIRUS TRANSCRIPTOME REVEALS TEMPORAL GENE EXPRESSION PROFILE AND HOST TRANSCRIPTIONAL SHIFT
Authors
  • Rodrigo Araújo Lima Rodrigues
  • Amina Cherif Louazani
  • Agnello Picorelli
  • Graziele P. Oliveira
  • Francisco Pereira Lobo
  • Philippe Colson
  • Bernard La Scola
  • Jônatas Santos Abrahão
Modality
Resumo
Subject area
Básica
Publishing Date
04/01/2021
Country of Publishing
Brasil
Language of Publishing
Inglês
Paper Page
https://www.even3.com.br/anais/cbv/282152-analysis-of-a-marseillevirus-transcriptome-reveals-temporal-gene-expression-profile-and-host-transcriptional-shif
ISBN
978-65-5941-175-7
Keywords
Giant virus, marseillevirus, transcriptome, RNA-seq
Summary
Marseilleviruses comprise a family of large double-stranded DNA viruses belonging to the proposed order “Megavirales”. These viruses have a circular genome of ~370 kbp, coding hundreds of genes. Over a half of their genes are associated with AT-rich putative promoter motifs, which have been demonstrated to be important for gene regulation. However, the transcriptional profile of Marseilleviruses is currently unknown. Here we used RNA sequencing technology to get a general transcriptional profile of Marseilleviruses. Eight million 75-bp-long nucleotide sequences were robustly mapped to all 457 genes initially predicted for Marseillevirus isolate T19, the prototype strain of the family, and we were able to assemble 359 viral contigs using a genome-guided approach with stringent parameters. These reads were differentially mapped to the genes according to the replicative cycle time point from which they were obtained. Cluster analysis indicated the existence of three main temporal categories of gene expression, early, intermediate and late, which were validated by quantitative reverse transcription polymerase chain reaction assays targeting several genes. Genes belonging to different functional groups exhibited distinct expression levels throughout the infection cycle. We observed that the previously predicted promoter motif, AAATATTT, as well as new predicted motifs, were not specifically related to any of the temporal or functional classes of genes, suggesting that other components are involved in temporally regulating virus transcription. Moreover, the host transcription machinery is heavily altered, and many genes are down regulated, including those related to translation process. This study provides an overview of the transcriptional landscape of Marseilleviruses.
Title of the Event
XXXI Congresso Brasileiro de Virologia & XV Encontro de Virologia do Mercosul
Title of the Proceedings of the event
Anais do Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul
Name of the Publisher
Even3
Means of Dissemination
Meio Digital
DOI

How to cite

RODRIGUES, Rodrigo Araújo Lima et al.. ANALYSIS OF A MARSEILLEVIRUS TRANSCRIPTOME REVEALS TEMPORAL GENE EXPRESSION PROFILE AND HOST TRANSCRIPTIONAL SHIFT.. In: Congresso Brasileiro de Virologia & Encontro de Virologia do Mercosul. Anais...Porto Alegre(RS) Online, 2020. Available in: https//www.even3.com.br/anais/cbv/282152-ANALYSIS-OF-A-MARSEILLEVIRUS-TRANSCRIPTOME-REVEALS-TEMPORAL-GENE-EXPRESSION-PROFILE-AND-HOST-TRANSCRIPTIONAL-SHIF. Access in: 08/08/2025

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