A GENOME-WIDE ASSOCIATION STUDY FOR RESISTANCE TO PSEUDOMONAS SYRINGAE PV. GARCAE IN COFFEA ARABICA PROVIDES A SUITABLE MARKER FOR ASSISTED SELECTION

Caroline Ariyoshi; Gustavo César Sant'ana; Mariane Silva Felicio; Gustavo Hiroshi Sera; Lucas Mateus Rivero Rodrigues; Filipe Gimenez Carvalho; Livia mAria nOgueira BRito; Luciana Harumi Shigueoka; Suzete Aparecida Lanza Destéfano; Luiz Filipe Protasio Pereira

Título do Trabalho

Autores

Caroline Ariyoshi
Gustavo César Sant'ana
Mariane Silva Felicio
Gustavo Hiroshi Sera
Lucas Mateus Rivero Rodrigues
Filipe Gimenez Carvalho
Livia mAria nOgueira BRito
Luciana Harumi Shigueoka
Suzete Aparecida Lanza Destéfano
Luiz Filipe Protasio Pereira

Modalidade

Prêmio

Área temática

Biotecnologia e Genômica

Data de Publicação

16/02/2022

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/cbmp2021/436847-a-genome-wide-association-study-for-resistance-to-pseudomonas-syringae-pv-garcae-in-coffea-arabica-provides-a-su

ISBN

978-65-5941-579-3

Palavras-Chave

GWAS, plant disease, Marker Assisted Selection, coffee breeding

Resumo

Bacteria Halo Blight (BHB), a coffee plant disease caused by Pseudomonas syringae pv. garcae, has been gaining importance in producing mountain regions and in mild temperatures areas as well in coffee nurseries. Its control, by the application of copper/antibiotics, can cause environmental problems and increase production costs. The development and use of BHB resistant cultivars is the most economical and sustainable alternative. However, most of C. arabica cultivars are susceptible to this disease. In contrast, a greater resistance source is found in C. arabica wild type plants from Ethiopia, center of origin of species. Thus, exploring the genetic diversity of a wild C. arabica collection we performed a Genome-Wide Association Study (GWAS) for identify alleles associated with resistance to BHB. Further, from the GWAS results, we developed and validate primers for Marker Assisted Selection (MAS) using Allele Specific-Polymerase Chain Reaction (AS-PCR). For the GWAS, we used genotyping by sequencing (GBS) data of 120 C. arabica wild type accessions, 11 C. arabica cultivars and the BA-10 access. And for the primer AS-PCR validation, we used the DNA samples of a backcross (BC) (n = 38) and of a F2 (n = 138) C. arabica breeding populations segregating for resistance to BHB. All accessions used in this work were phenotyped for resistance to BHB, based on a scale ranging from 0 to 5, where 0 corresponds to qualitative resistance. From the GBS data, aligned to the C. arabica genome, 11.290 SNPs were selected. Through the GWAS, using single-locus and multi-locus association models, 13 alleles associated with resistance to BHB were identified. In linkage disequilibrium with these alleles, 14 candidate genes related to response to biotic stress were found. Among them, one is predicted to encoding a serine/threonine-kinase and another one a CC-NBS-LRR. These two genes were validated through gene expression and are probably involved in specific recognition of pathogen by the plant and in the activation of hypersensitivity response (HR). The primers AS-PCR for the allele with the highest associative value and linked to the CC-NBS-LRR gene was able to identify accessions, in the breeding populations, with qualitative resistance. The linkage between the amplification corresponding to those primers AS-PCR and qualitative resistance showed 93.75 % of accuracy. In addition, it was possible to identify that the genetic control of qualitative resistance to BHB is probably conferred by a dominant locus. Through the GWAS was possible to characterize a genomic region involved in HR response which increase our knowledge about C. arabica and P. syringae pv. garcae interaction and highlights a potential target for gene editing. Last, this work provides a molecular marker based in a simple PCR. It is a low-cost method that can be easily implemented supporting C. arabica breeding programs aiming resistance to this disease.

Título do Evento: 11º Congresso Brasileiro de Melhoramento de Plantas
Título dos Anais do Evento: Anais 11º Congresso Brasileiro de Melhoramento de Plantas.
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

ARIYOSHI, Caroline et al.. A GENOME-WIDE ASSOCIATION STUDY FOR RESISTANCE TO PSEUDOMONAS SYRINGAE PV. GARCAE IN COFFEA ARABICA PROVIDES A SUITABLE MARKER FOR ASSISTED SELECTION.. In: Anais 11º Congresso Brasileiro de Melhoramento de Plantas.. Anais...Pelotas(RS) UFPel, 2021. Disponível em: https//www.even3.com.br/anais/cbmp2021/436847-A-GENOME-WIDE-ASSOCIATION-STUDY-FOR-RESISTANCE-TO-PSEUDOMONAS-SYRINGAE-PV-GARCAE-IN-COFFEA-ARABICA-PROVIDES-A-SU. Acesso em: 25/06/2025