Annals of the Brazilian Congress of Plant Physiology and Ibero-latinamerican Congress of Plant Biology

THE INNARDS OF THE CELL: STUDIES OF WATER DIPOLAR RELAXATION USING ACDAN AND PRODAN FLUORESCENT PROBES.

Published in 26/09/2022 - ISBN: 978-65-5941-830-5

Link All Papers
LinkSubmission
Paper Title
THE INNARDS OF THE CELL: STUDIES OF WATER DIPOLAR RELAXATION USING ACDAN AND PRODAN FLUORESCENT PROBES.
Authors
  • Manuel Cabadas
  • Ramiro Hernán Lascano
  • Leonel Malacrida
  • Santiago Nicolas Otaiza Gonzales
  • Germán Robert
Modality
Abstract
Subject area
Plant Physiology
Publishing Date
26/09/2022
Country of Publishing
Brasil
Language of Publishing
Inglês
Paper Page
https://www.even3.com.br/anais/cbfv2022_registration/516284-the-innards-of-the-cell--studies-of-water-dipolar-relaxation-using-acdan-and-prodan-fluorescent-probes
ISBN
978-65-5941-830-5
Keywords
Association Induction Hypothesis, Arabidopsis, DAN probes, Dipolar relaxation, Water activity
Summary
Water is the major and principal component of all living cells. In plant cells, it is known to account for approximately 90% of their total weight. The role assigned to water in living systems has changed over time. Many of the properties of water change drastically when measured in living systems compared to in vitro solutions. In our experiments, we have used the fluorophores ACDAN and PRODAN (2 of the 3 members of the DAN probes series) to measure changes in the dipolar relaxation of water in root cells (elongation zone, meristematic zone and root cap) and leaves (stomata) of Arabidopsis thaliana. These molecules, widely characterized in biomimetic systems, respond to changes in the polarity of the environment. What is observed are changes in the degree of intracellular water dipolar relaxation between different tissues, and between different subcellular structures, evidence that supports the existence of heterogeneities in the degree of intracellular water dipolar relaxation in plant cells. In addition, we observed a dose-dependent effect on the degree of intracellular water dipolar relaxation under oxidative stress conditions, being able to identify lethal and sublethal doses of ROS. These results are interpreted considering an alternative view of cellular physiology: The Association-Induction Hypothesis (AIH). This theory is a theoretical framework that emphasizes the structure of intracellular water in the maintenance of the living state in cells.
Title of the Event
XVIII Brazilian Congress of Plant Physiology and I Ibero-latinoamerican Congress of Plant Biology
City of the Event
Porto Alegre
Title of the Proceedings of the event
Annals of the Brazilian Congress of Plant Physiology and Ibero-latinamerican Congress of Plant Biology
Name of the Publisher
Even3
Means of Dissemination
Meio Digital

How to cite

CABADAS, Manuel et al.. THE INNARDS OF THE CELL: STUDIES OF WATER DIPOLAR RELAXATION USING ACDAN AND PRODAN FLUORESCENT PROBES... In: Annals of the Brazilian Congress of Plant Physiology and Ibero-latinamerican Congress of Plant Biology. Anais...Porto Alegre(RS) CEPUCRS, 2022. Available in: https//www.even3.com.br/anais/cbfv2022_registration/516284-THE-INNARDS-OF-THE-CELL--STUDIES-OF-WATER-DIPOLAR-RELAXATION-USING-ACDAN-AND-PRODAN-FLUORESCENT-PROBES. Access in: 07/05/2025

Paper

Even3 Publicacoes