MULTI-OMICS ANALYSIS OF MICROALGAE UNDER NITROGEN DEPRIVATION REVEALED NOVEL ARGINASE-LIKE FUNCTION ASSOCIATED TO THE CONTROL OF GROWTH AND LIPIDS OVERACCUMULATION

Lais Albuquerque Giraldi; Lucca de Filipe Rebocho Monteiro; Diego Mauricio Riaño Pachón; Alexandre Victor Fassio; Ivan Alberto Sandoval Salazar; Aline Cristina de Camargo; Valéria Lopes Ferreira Vianna; Camila Caldana; Prof. Dra. Flavia Vischi Winck

Paper Title

Authors

Lais Albuquerque Giraldi
Lucca de Filipe Rebocho Monteiro
Diego Mauricio Riaño Pachón
Alexandre Victor Fassio
Ivan Alberto Sandoval Salazar
Aline Cristina de Camargo
Valéria Lopes Ferreira Vianna
Camila Caldana
Prof. Dra. Flavia Vischi Winck

Modality

Abstract

Subject area

Plant Physiology

Publishing Date

26/09/2022

Country of Publishing

Brasil

Language of Publishing

Inglês

Paper Page

https://www.even3.com.br/anais/cbfv2022_registration/516205-multi-omics-analysis-of-microalgae-under-nitrogen-deprivation-revealed-novel-arginase-like-function-associated-to

ISBN

978-65-5941-830-5

Keywords

proteomics, metabolomics, amino acids, signaling, systems biology

Summary

The cellular metabolism of microalgae species is known to be highly complex, due to their evolutionary history and the genetic background of their ancestral prokaryotic donor and eukaryotic host species, which allow many of these organisms to survive in photoautotrophic, mixotrophic or even heterotrophic metabolic modes. Microalgae from the class of Chlorophyceae under photoautotrophic and mixotrophic conditions, have metabolic pathways highly conserved in land plants. Nevertheless, several microalgae species can over accumulate neutral lipids, specially triacylglycerols, under nitrogen deprivation conditions. This phenotype is associated to reduced cell growth, protein synthesis, Photosystem II and chlorophyll content, which impacts microalgae biotechnological applications. Therefore, we have investigated time-resolved changes of the proteome, lipidome and metabolome profiles of Chlamydomonas reinhardtii cells using high-resolution Mass Spectrometry and integrative data analysis using Manifold clustering methods. We observed the modulation of abundance of the amino acids Arginine and Ornithine and alterations in the protein abundance of enzymes from their catabolism and anabolism, respectively. The data analysis indicated that this metabolic shift may be part of a signaling cascade and it is likely controlled by an enzyme with an Arginase function, even though Arginase enzyme is not conserved at the genomic level in Chlamydomonas. We tested Arginase inhibitors (L-Valine, L-Ornithine and Norvaline) in non-toxic levels in the cells under mixotrophic conditions and confirmed the enhanced lipids accumulation without affecting cell growth performance. Further studies on this novel Arginase function are on progress and may reveal novel routes for amino acids metabolism in microalgae.

Title of the Event: XVIII Brazilian Congress of Plant Physiology and I Ibero-latinoamerican Congress of Plant Biology
City of the Event: Porto Alegre
Title of the Proceedings of the event: Annals of the Brazilian Congress of Plant Physiology and Ibero-latinamerican Congress of Plant Biology
Name of the Publisher: Even3
Means of Dissemination: Meio Digital

How to cite

GIRALDI, Lais Albuquerque et al.. MULTI-OMICS ANALYSIS OF MICROALGAE UNDER NITROGEN DEPRIVATION REVEALED NOVEL ARGINASE-LIKE FUNCTION ASSOCIATED TO THE CONTROL OF GROWTH AND LIPIDS OVERACCUMULATION.. In: Annals of the Brazilian Congress of Plant Physiology and Ibero-latinamerican Congress of Plant Biology. Anais...Porto Alegre(RS) CEPUCRS, 2022. Available in: https//www.even3.com.br/anais/cbfv2022_registration/516205-MULTI-OMICS-ANALYSIS-OF-MICROALGAE-UNDER-NITROGEN-DEPRIVATION-REVEALED-NOVEL-ARGINASE-LIKE-FUNCTION-ASSOCIATED-TO. Access in: 28/06/2025