Annals of the Brazilian Congress of Plant Physiology and Ibero-latinamerican Congress of Plant Biology

INTERACTION OF FREE AND NANOENCAPSULATED NITRIC OXIDE DONORS WITH THE ROOTS OF SOYBEAN PLANTS GROWN IN HYDROPONICS

Published in 26/09/2022 - ISBN: 978-65-5941-830-5

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Paper Title
INTERACTION OF FREE AND NANOENCAPSULATED NITRIC OXIDE DONORS WITH THE ROOTS OF SOYBEAN PLANTS GROWN IN HYDROPONICS
Authors
  • Ana Cristina Preisler
  • Telma S. Santos
  • Joana Claudio Pieretti
  • Amedea Barozzi Seabra
  • Waldiceu Verri Jr
  • Halley Caixeta de Oliveira
Modality
Abstract
Subject area
Plant Physiology
Publishing Date
26/09/2022
Country of Publishing
Brasil
Language of Publishing
Inglês
Paper Page
https://www.even3.com.br/anais/cbfv2022_registration/515011-interaction-of-free-and-nanoencapsulated-nitric-oxide-donors-with-the-roots-of-soybean-plants-grown-in-hydroponic
ISBN
978-65-5941-830-5
Keywords
Nanoparticle, S-nitrosoglutathione, Soybean
Summary
Nitric oxide (NO) is a molecule that participates in several processes of plant growth and development, in addition to acting in defense against stress. The exogenous application of NO in plants is limited, requiring the use of NO donors, such as S-nitrosoglutathione (GSNO). However, these compounds present relative instability. Thus, the use of nanocarriers appears as a promising alternative for the application of NO donors in agriculture. However, there is still a gap related to the interaction mechanisms between nanomaterials and plants. Therefore, the objective of this study was to evaluate the effects of free and nanoencapsulated GSNO in altering the bioavailability of NO in the roots of soybean plants. The experiment was conducted in hydroponics using Glycine max L. Merr. (BRS 257). The treatments were control (distilled water), chitosan/tripolyphosphate nanoparticles (CS/TPP NPs) containing GSNO, CS/TPP NPs without GSNO, and free GSNO for each time interval (30 min and 24 h). The nanoparticles were labeled with rhodamine B to allow their detection in the roots by confocal spectral microscopy. The NO levels in the roots were measured simultaneously with the use of the fluorescent probe DAF-2DA. The treatment with GSNO-loaded NPs induced higher NO levels in the roots compared to the other treatments, particularly in the 30 min time-point. This fluorescence was quenched by the NO scavenger cPTIO, which corroborates that it was related to NO. It was possible to detect the fluorescence from rhodamine inside the roots treated with nanoparticles in both time-points, although it increased after 24 h. There was a high percentage of colocalization between the NO and rhodamine fluorescence in the roots. Overall, these results suggest that the CS/TPP NPs were absorbed by the roots, resulting in a more efficient NO delivery to the tissues than the free GSNO.
Title of the Event
XVIII Brazilian Congress of Plant Physiology and I Ibero-latinoamerican Congress of Plant Biology
City of the Event
Porto Alegre
Title of the Proceedings of the event
Annals of the Brazilian Congress of Plant Physiology and Ibero-latinamerican Congress of Plant Biology
Name of the Publisher
Even3
Means of Dissemination
Meio Digital

How to cite

PREISLER, Ana Cristina et al.. INTERACTION OF FREE AND NANOENCAPSULATED NITRIC OXIDE DONORS WITH THE ROOTS OF SOYBEAN PLANTS GROWN IN HYDROPONICS.. In: Annals of the Brazilian Congress of Plant Physiology and Ibero-latinamerican Congress of Plant Biology. Anais...Porto Alegre(RS) CEPUCRS, 2022. Available in: https//www.even3.com.br/anais/cbfv2022_registration/515011-INTERACTION-OF-FREE-AND-NANOENCAPSULATED-NITRIC-OXIDE-DONORS-WITH-THE-ROOTS-OF-SOYBEAN-PLANTS-GROWN-IN-HYDROPONIC. Access in: 08/05/2025

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