Annals of the Brazilian Congress of Plant Physiology and Ibero-latinamerican Congress of Plant Biology

MIR156/SQUAMOSA PROTEIN LIKE (SPL) HUB IN ANNATTO (BIXA ORELLANA L.): PETER PAN PLANTS LESSENING BIXIN.

Published in 26/09/2022 - ISBN: 978-65-5941-830-5

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Paper Title
MIR156/SQUAMOSA PROTEIN LIKE (SPL) HUB IN ANNATTO (BIXA ORELLANA L.): PETER PAN PLANTS LESSENING BIXIN.
Authors
  • Kleiton Lima de Godoy Machado
  • Marcos Bruno Silva Duarte
  • Lázara Aline Simões Silva
  • Daniele V. Faria
  • Fábio T. S. Nogueira
  • Wagner C. Otoni
Modality
Abstract
Subject area
Plant Physiology
Publishing Date
26/09/2022
Country of Publishing
Brasil
Language of Publishing
Inglês
Paper Page
https://www.even3.com.br/anais/cbfv2022_registration/511771-mir156squamosa-protein-like-(spl)-hub-in-annatto-(bixa-orellana-l)--peter-pan-plants-lessening-bixin
ISBN
978-65-5941-830-5
Keywords
microRNA; Phase change; Plant development; Secondary metabolism.
Summary
Annatto [Bixa orellana L. (Bixaceae)] is a woody plant species with a fast growth pattern. Annatto produces a main apocaterotenoid namely bixin, a unique natural dye. This compound is highly used in diverse industries. Despite Annatto relevance, there are several gaps for the understanding of its developmental processes. The most interesting is the role of miR156/Squamosa Protein Like module in its physiology. The SPL proteins are responsible for integrating plant physiology development through modulation of plant morphology, anatomy, and secondary compounds biosynthesis. Therefore, we aimed to assess phenotypic changes, bixin and ABA production, expression of bixin-linked genes, and proteome portray in SPL proteins defective lines of B. orellana plants through overexpression of miR156 (OE::156). Consequently, these plants are not able to change vegetative phases. We observed new patterns in plant architecture and leaf blade features such as number and length of leaves, diameter, and plant height. In addition, higher ABA levels were found in OE::156 plants compared to not-transformed plants (Nt). Moreover, OE::156 plants displayed lower bixin content and lesser bixin channels than Nt. Furthermore, OE::156 lines showed lower levels of SABBATH and ALDH3H1, which are key genes in the bixin biosynthesis. Moreover, OE::156 lines displayed lower CCD4 expression in contrast to higher CCD1 transcriptional levels. Besides that, the proteome showed ZEP upaccumulation in OE::156 plants, which indicates that carbons are being translocated from bixin to ABA production. In addition, the proteome revealed that OE::156 plants showed downaccumulation of proteins related to secondary metabolites biosynthesis, indicating a dependent SPL protein route. Therefore, we suggested that bixin production is dependent of Annatto plant age since juvenilized plants allocated carbon in ABA biosynthesis instead of bixin biogenesis.
Title of the Event
XVIII Brazilian Congress of Plant Physiology and I Ibero-latinoamerican Congress of Plant Biology
City of the Event
Porto Alegre
Title of the Proceedings of the event
Annals of the Brazilian Congress of Plant Physiology and Ibero-latinamerican Congress of Plant Biology
Name of the Publisher
Even3
Means of Dissemination
Meio Digital

How to cite

MACHADO, Kleiton Lima de Godoy et al.. MIR156/SQUAMOSA PROTEIN LIKE (SPL) HUB IN ANNATTO (BIXA ORELLANA L.): PETER PAN PLANTS LESSENING BIXIN... In: Annals of the Brazilian Congress of Plant Physiology and Ibero-latinamerican Congress of Plant Biology. Anais...Porto Alegre(RS) CEPUCRS, 2022. Available in: https//www.even3.com.br/anais/cbfv2022_registration/511771-MIR156SQUAMOSA-PROTEIN-LIKE-(SPL)-HUB-IN-ANNATTO-(BIXA-ORELLANA-L)--PETER-PAN-PLANTS-LESSENING-BIXIN. Access in: 20/06/2025

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