Proceedings of the 8th Brazilian Congress of Biotechnology

ANTIGLYCANT POTENTIAL AND PROTECTIVE EFFECTS OF PICEATANNOL ON HUMAN HEMOGLOBIN: STRUCTURAL AND THERMODYNAMIC INSIGHTS

Published in 21/11/2025 - ISBN: 978-65-272-1865-4

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Paper Title
ANTIGLYCANT POTENTIAL AND PROTECTIVE EFFECTS OF PICEATANNOL ON HUMAN HEMOGLOBIN: STRUCTURAL AND THERMODYNAMIC INSIGHTS
Authors
  • Tauane dos Santos Rocha
  • Rosylene P. C. Lopes
  • Wallison dos Santos Dias
  • Iara Barros Valentim
  • Julio Cosme Santos da Silva
  • Josué Carinhanha Caldas Santos
  • Marília Goulart
  • Jadriane de Almeida Xavier
Modality
Abstract
Subject area
Applications of Biotechnology in Human Health (vaccine and drug development);
Publishing Date
21/11/2025
Country of Publishing
Brazil | Brasil
Language of Publishing
en-US
Paper Page
https://www.even3.com.br/anais/biotecnologia2025/1253635-antiglycant-potential-and-protective-effects-of-piceatannol-on-human-hemoglobin--structural-and-thermodynamic-in
ISBN
978-65-272-1865-4
Keywords
Hemoglobin, Antiglycation, Piceatannol, Soret, ThT, AGEs
Summary
Piceatannol (PIC) is a stilbene compound structurally analogous to resveratrol and belongs to a broad group of natural defense polyphenols found in various plant species, such as grapes, blueberries, and sugarcane. It is a major bioactive constituent of yellow passion fruit (Passiflora edulis) seeds, exhibiting antioxidant, antiglycation, and fibrillation-inhibitory activities, particularly relevant against degenerative and metabolic disorders such as Alzheimer’s disease and diabetes. One of the key biochemical pathways leading to these conditions is the formation of advanced glycation end products (AGEs) through non-enzymatic reactions, such as the Maillard reaction, between reducing sugars and nucleophilic groups of biomolecules. As such, the objective of this work was to obtain the progression or decrease of Hb’s glycation, through the measurement of AGEs fluorescent, by analysis of the structural changes in the Soret band of Hb, by the formation of Hb aggregates, in the presence of PIC (10 – 300 µM) and using glucose and glucose-fructose mixture as reducing sugars in the process, performed over a period of 5 weeks. Additionally, Hb–PIC interactions were characterized by fluorescence spectroscopy at different temperatures (298, 304, and 310 K), and molecular docking simulations were performed to predict binding modes and affinities. As a result, PIC inhibited Hb glycation by 88% and 81% when Hb was incubated with glucose and with a glucose–fructose mixture, respectively, at a concentration of 300 µM. Analysis of the Soret band and ThT fluorescence revealed clear protective effects of PIC against alterations in the Hb heme group and the formation of amyloid fibrils. PIC prevented heme alterations by up to 23% and reduced amyloid fibril formation by up to 52%, underscoring its ability to counteract glycation-induced conformational damage. Thermodynamic analysis revealed a spontaneous interaction between PIC and Hb (ΔG = –24.04 kJ.mol⁻¹), supported by docking predictions indicating a favorable binding free energy (–27 kJ.mol⁻¹) primarily driven by electrostatic interactions. According to these results, indicate that PIC effectively inhibits protein glycation, preserves hemoglobin structure, and limits amyloid fibrillation, suggesting its potential as a therapeutic agent for preventing glycation-associated complications, especially those related to diabetes and neurodegenerative diseases. Acknowledgments and funding sources: RENORBIO, LEEO, UFAL, CAPES, FAPEAL.
Title of the Event
8th Brazilian Biotechnology Congress.
City of the Event
Natal
Title of the Proceedings of the event
Proceedings of the 8th Brazilian Congress of Biotechnology
Name of the Publisher
Even3
Means of Dissemination
Meio Digital

How to cite

ROCHA, Tauane dos Santos et al.. ANTIGLYCANT POTENTIAL AND PROTECTIVE EFFECTS OF PICEATANNOL ON HUMAN HEMOGLOBIN: STRUCTURAL AND THERMODYNAMIC INSIGHTS.. In: Proceedings of the 8th Brazilian Congress of Biotechnology. Anais...Natal(RN) Hotel Praia Mar, 2025. Available in: https//www.even3.com.br/anais/biotecnologia2025/1253635-ANTIGLYCANT-POTENTIAL-AND-PROTECTIVE-EFFECTS-OF-PICEATANNOL-ON-HUMAN-HEMOGLOBIN--STRUCTURAL-AND-THERMODYNAMIC-IN. Access in: 04/04/2026

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