PHOSPHOPROTEOME ANALYSIS OF EUCALYPTUS EXPOSED TO DIFFERENT CO2 CONCENTRATIONS: FROM STIMULUS TO STRESS.

Marina de Lima Nogueira; Cassio Flávio Fonseca Lima; Naiara Scarabeli Zancanari; Guilherme Pansani Duarte; Ive De Smet; Tiago Santana Balbuena

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Trabalho

Título do Trabalho

PHOSPHOPROTEOME ANALYSIS OF EUCALYPTUS EXPOSED TO DIFFERENT CO2 CONCENTRATIONS: FROM STIMULUS TO STRESS.

Autores

Marina de Lima Nogueira
Cassio Flávio Fonseca Lima
Naiara Scarabeli Zancanari
Guilherme Pansani Duarte
Ive De Smet
Tiago Santana Balbuena

Modalidade

Resumo

Área temática

Biotecnologia e Genômica

Data de Publicação

21/09/2023

País da Publicação

Brasil

Idioma da Publicação

Português

Página do Trabalho

https://www.even3.com.br/anais/12cbmp/661676-phosphoproteome-analysis-of-eucalyptus-exposed-to-different-co2-concentrations--from-stimulus-to-stress

ISBN

978-85-5722-993-8

Palavras-Chave

Eucalyptus; CO2; mass spectrometry

Resumo

Eucalyptus plants exhibit rapid growth rates and possess a remarkable capacity for assimilating and sequestering substantial amounts of atmospheric carbon. It is predicted that the atmospheric CO2 concentration will increase substantially in the near future, providing a valuable source of carbon skeletons for fast-growing plants. However, surpassing a certain threshold of CO2 concentration can induce stress in plants and hamper their development. To gain a deeper understanding of this interplay between stimulation and stress, we conducted an experiment involving Eucalyptus hybrids seedlings: E. urophylla x E. grandis subjected to four distinct CO2 concentrations (420, 680, 980, and 1200 ppm) over a 12-day period in a growth chamber with light intensity varying between 3793-7196 lux by 12h light:12h dark. We evaluated plant growth (stem diameter, leaf count, shoot count, leaf area, plant height) and carried out a phosphoproteome analysis in leaf samples. Plants revealed a higher growth when cultivated at 680 and 980 ppm in relation to the control condition (420 ppm). Growth at 1200 ppm induced a smaller growth change in relation to 680 and 980 ppm, but a higher growth in relation to the control condition though. The phosphoproteome dataset identified a total of 3610 phosphorylated sites, from which a total of 2520 sites were confidently assigned. Pairwise comparisons against the control resulted in the identification of 59, 61 and 30 differentially regulated phosphorylated sites in the 680, 980 and 1200 ppm treatments, respectively. Proteins containing the differentially regulated sites in plants grown at 680 ppm were mostly involved in primary metabolic process (45.9%), response to stimulus (35.1%), and stress response (16.2%). Proteins involved in primary metabolic (28.2%) process also comprised most of the differentially regulated phosphosites in plants cultivated at 980 ppm, followed by proteins responsive to stimulus (16.2%) and stress (17.9%). The dataset from plants cultivated at 1200 ppm revealed that proteins involved in primary metabolic process did not present quantitative changes in the phosphosites, though 58.8% and 23.5% of the variable proteins were involved in response to stimulus and stress response. This preliminary data suggest a higher CO2-induced growth when plants are cultivated at 680 and 980 ppm. Conversely, cultivation at 1200 ppm of CO2 induced growth at a lower extent and higher stress responses in relation to the other CO2 concentrations.

Título do Evento: 12º Congresso Brasileiro de Melhoramento de Plantas 2023
Cidade do Evento: Caxambu
Título dos Anais do Evento: Anais do 12º Congresso Brasileiro de Melhoramento de Plantas
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

NOGUEIRA, Marina de Lima et al.. PHOSPHOPROTEOME ANALYSIS OF EUCALYPTUS EXPOSED TO DIFFERENT CO2 CONCENTRATIONS: FROM STIMULUS TO STRESS... In: Anais do 12º Congresso Brasileiro de Melhoramento de Plantas. Anais...Caxambu(MG) Hotel Glória, 2023. Disponível em: https//www.even3.com.br/anais/12cbmp/661676-PHOSPHOPROTEOME-ANALYSIS-OF-EUCALYPTUS-EXPOSED-TO-DIFFERENT-CO2-CONCENTRATIONS--FROM-STIMULUS-TO-STRESS. Acesso em: 16/07/2025