PHYLOGENETIC STUDY OF THE ENDOPHYTIC FUNGUS DIAPORTHE VOCHYSIAE

Published in 08/11/2023 - ISBN: 978-65-272-0061-1

Paper Title
PHYLOGENETIC STUDY OF THE ENDOPHYTIC FUNGUS DIAPORTHE VOCHYSIAE
Authors
  • Alicia Sofia Tempesta Brasil Silka
  • Desirrê Alexia Lourenço Petters Vandresen
  • CHIRLEI GLIENKE
  • Amanda Inça
Modality
Poster
Subject area
Phylogeny and Evolution
Publishing Date
08/11/2023
Country of Publishing
Brazil | Brasil
Language of Publishing
Inglês
Paper Page
https://www.even3.com.br/anais/xmeeting2023/643265-phylogenetic-study-of-the-endophytic-fungus-diaporthe-vochysiae
ISBN
978-65-272-0061-1
Keywords
Diaporthe vochysiae; phylogeny
Summary
The recently described fungus Diaporthe vochysiae was originally isolated as an endophyte from the medicinal plants Vochysia divergens and Styphnodendron adstringens of the Pantanal and Cerrado biomes, respectively. In bioprospecting studies, D. vochysiae isolates likely represent promising sources of novel and bioactive compounds, since the type-strain of this species (LGMF1583 / CMRP3911) was described to produce two novel carboxamides (vochysiamides A and B). D. vochysiae is a genetically and macro-morphologically diverse species with 61 known isolates housed at the NAPI CMRP/Taxonline collection, and this genetic and morphological diversity could be correlated with a potential diversity in the bioactivity profile of the compounds produced by the isolates. In this sense, a detailed investigation of the genetic diversity and proper delimitation of species boundaries is an important step to better understand D. vochysiae species and guide further bioprospecting approaches. Multigene phylogenetic analyses were performed using a concatenated alignment including TEF (translation elongation factor), TUB (?? tubulin) CAL (calmodulin) and HIS (histone 3) sequences,, under maximum likelihood and Bayesian inference methods, so that the hypothesis could have the robustness of Bayesian inference (with posterior probability values) and maximum likelihood (with bootstrap values), delimiting the Diaporthe species. Analyses included representative strains from the closest sister species to D. vochysiae, which all belong to the recently proposed monophyletic Diaporthe sojae clade, with Diaporthe ganjae as outgroup. As Diaporthe is described a highly diverse genus, working with species complexes or ingroup clades (such as Diaporthe sojae clade) is usually recommended for better species delimitation, as analyses including only closer taxa are thought to facilitate the resolution of intraspecific relations and relationships among very closely related taxa. The results so far indicate that there are likely two groups within the larger monophyletic D. vochysiae sensu lato group, which could indicate the existence of more than one species. The first group, which contains the type strain (LGMF1583 /CMRP3911) is apparently polytomic and not very diverse, while the second group appears to be genetically diverse. In order to assess if the status of such groups as different species or subspecies, further analyses combining haplotype evaluation, coalescent approaches, metabolite profiling, and bioactivity assays will be performed. Moreover, representative isolates from both ingroups observed in D. vochysiae sensu lato will have their genome sequenced for the characterization of biosynthetic gene clusters, which will enable further comparisons and a better evaluation of their bioactive potential.
Title of the Event
X-Meeting / BSB 2023
City of the Event
Curitiba
Title of the Proceedings of the event
X-Meeting presentations
Name of the Publisher
Even3
Means of Dissemination
Meio Digital

How to cite

SILKA, Alicia Sofia Tempesta Brasil et al.. PHYLOGENETIC STUDY OF THE ENDOPHYTIC FUNGUS DIAPORTHE VOCHYSIAE.. In: X-Meeting presentations. Anais...Curitiba(PR) Campus da indústria, 2023. Available in: https//www.even3.com.br/anais/xmeeting2023/643265-PHYLOGENETIC-STUDY-OF-THE-ENDOPHYTIC-FUNGUS-DIAPORTHE-VOCHYSIAE. Access in: 20/06/2025

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