X-Meeting presentations

GENOMICS OF MARINE BACTERIA FROM EXTREME ENVIRONMENTS OF ANTARCTICA

Published in 08/11/2023 - ISBN: 978-65-272-0061-1

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Paper Title
GENOMICS OF MARINE BACTERIA FROM EXTREME ENVIRONMENTS OF ANTARCTICA
Authors
  • Rayana dos Santos Feltrin
  • Alan M. Durham
  • Enrique Rozas
  • Claudio Augusto Oller do Nascimento
  • Renatta Santos de Oliveira
  • Rodrigo da Silva Galhardo
  • Carlos Frederico Martins Menck
Modality
Poster
Subject area
DNA and Genomics
Publishing Date
08/11/2023
Country of Publishing
Brazil | Brasil
Language of Publishing
en-US
Paper Page
https://www.even3.com.br/anais/xmeeting2023/641440-genomics-of-marine-bacteria-from-extreme-environments-of-antarctica
ISBN
978-65-272-0061-1
Keywords
extreme environments, Antarctica, DNA repair
Summary
The Antarctic continent keeps several extreme environmental conditions and specific isolation from the effects of human society. In that place, the genetic material of the organisms is under constant aggression, with various agents that damage the DNA molecule, including the ultraviolet radiation (UV) from sunlight. Other environmental conditions can also challenge the maintenance of DNA stability, such as the very low temperatures, making Antarctica an interesting place to study how organisms maintain genome integrity. In this work, we have sequenced the genomes of three Antarctic marine bacteria, two isolated from sediment samples from a depth of 280 m and the third from 1500 m (samples obtained at the Bransfield Strait). The greater the depth conditions in the sea, the bacteria endure higher concentrations of CO2 under higher atmospheric pressure. The DNA from these isolates was extracted and sequenced by the Illumina platform. De novo genome assembly was performed with SPAdes v3.15.4. Assembly metrics were assessed with QUAST v5.2.0 and genome completeness was analyzed by BUSCO v5.3.1. Additionally, annotation was conducted using the Prokaryotic Genome Annotation Pipeline (PGAP) and species identification was done based on 16S ribosomal RNA (rRNA) sequence and using the software GTDB-Tk 2.1.0. Functional annotation was assessed by eggNOG. Also, we have analyzed the presence of DNA repair genes of these organisms using tblastn. Species identification has revealed that the species are Bacillus siamensis, Rossellomorea vietnamensis, and Ralstonia pickettii. Preliminary results indicate that most of the protein functions of these bacteria are unknown. Moreover, we have found that most of the DNA repair genes are present in these bacteria, pointing out that there are many ways for them to deal with DNA damage. As a perspective, we intend to do a transcriptome of R. picketti, the 1500 m-bacteria, in two conditions: one with atmospheric pressure and no saturation of CO2 and other with 100 atm and 100% of CO2, to know which genes are more expressed in the last condition.
Title of the Event
X-Meeting / BSB 2023
City of the Event
Curitiba
Title of the Proceedings of the event
X-Meeting presentations
Name of the Publisher
Even3
Means of Dissemination
Meio Digital

How to cite

FELTRIN, Rayana dos Santos et al.. GENOMICS OF MARINE BACTERIA FROM EXTREME ENVIRONMENTS OF ANTARCTICA.. In: X-Meeting presentations. Anais...Curitiba(PR) Campus da indústria, 2023. Available in: https//www.even3.com.br/anais/xmeeting2023/641440-GENOMICS-OF-MARINE-BACTERIA-FROM-EXTREME-ENVIRONMENTS-OF-ANTARCTICA. Access in: 04/04/2026

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