NOVEL PILZN-PILZ FUSION FAMILY AND SPIROCHAETAL PILZ CENSUS

Rodolfo Alvarenga Ribeiro; Aline Biazola Visnardi; Cristiane Rodrigues Guzzo Carvalho; Robson Francisco de Souza

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Submission

Paper Title

NOVEL PILZN-PILZ FUSION FAMILY AND SPIROCHAETAL PILZ CENSUS

Authors

Rodolfo Alvarenga Ribeiro
Aline Biazola Visnardi
Cristiane Rodrigues Guzzo Carvalho
Robson Francisco de Souza

Modality

Poster

Subject area

Phylogeny and Evolution

Publishing Date

08/11/2023

Country of Publishing

Brazil | Brasil

Language of Publishing

Inglês

Paper Page

https://www.even3.com.br/anais/xmeeting2023/638516-novel-pilzn-pilz-fusion-family-and-spirochaetal-pilz-census

ISBN

978-65-272-0061-1

Keywords

PilZ, Lineage Specific Expansion, Census

Summary

Leptospirales family has a major expansion in PilZ family members, in comparison to other Spirochetes, which contain up to 3 orthologs of PilZ fusions, whilst Leptospirales such as Leptospira, Turneriella and Leptonema have up to 7 orthologs of PilZ fusions and maintain the high of other PilZ family members as all Spirochetes have. DUF1577 is a member of PilZ fusion member, that includes PilZN+GAF+PilZ architecture, in which the GAF portion is interrupted by the first beta-sheet of PilZ, what could indicate a insertion from known PilZN-PilZ( flagellar regulator, MrkH), which controls motility of the flagella modulated by cytosolic c-di-GMP concentration. Spirochetales present several PilZ fusions such as MrkH-like, NpzN (N-terminal helical region PilZ transmembrane protein), and PilZ+PilZ proteins. In Leptospira interrogans there are at least 6 paralogs of DUF1577, which is found only in Spirochete phylum. In order to retrieve every PilZ family member, a search containing pfam PilZ models and our group PilZ models were used as seed, also considering the whole DUF1577 proteins from Leptospira as seed to jackhmmer iterative searches against a 1256 Spirochetal genome database. The resulting sequences were clustered with MMseqs2 to remove redundancy, and groups of 80 % coverage and 10e-3 evalue were aligned to contain their 80 % coverage 70 % identity representative proteins. Analysing those clusters, we were able to expand the detection of DUF1577, by splitting into its subdomains, characterized as PilZN, PilZ and GAF also building a wider model for PilZ including the divergent GAF insertion. Some MrkH fusions presented a unknown N-terminal extension, here named NpzN, that is predicted structurally to interact with the membrane. Dali searches were made in order to establish the fold of FMN-binding from DUF1577 model, showing that searches for the whole protein only gather proteins similar to MrkH, that have a conserved PilZN adjacent to a PilZ domain, but with no matches for the GAF insertion, except when only the GAF insertion was the search query, although the N-terminal of the insertion is not detected, only the last 2 a-helices were overall retrieved from the GAF family. Further iterative searches showed a connection between PilZN and PilZ, but none retrieved the GAF(PAS-like) insertion. DUF1577 orthologs were thoroughly annotated ranging only from 3 to 8 paralogs in Spirochetes, presuming it’s origin occurred in the root of spirochetal lineages, and recent duplications are shown in most Leptospirales, mainly on pathologic strains, which accumulate up to 3 fold PilZ family member proteins as of Spirochetales other lineages.

Title of the Event: X-Meeting / BSB 2023
City of the Event: Curitiba
Title of the Proceedings of the event: X-Meeting presentations
Name of the Publisher: Even3
Means of Dissemination: Meio Digital

How to cite

RIBEIRO, Rodolfo Alvarenga et al.. NOVEL PILZN-PILZ FUSION FAMILY AND SPIROCHAETAL PILZ CENSUS.. In: X-Meeting presentations. Anais...Curitiba(PR) Campus da indústria, 2023. Available in: https//www.even3.com.br/anais/xmeeting2023/638516-NOVEL-PILZN-PILZ-FUSION-FAMILY-AND-SPIROCHAETAL-PILZ-CENSUS. Access in: 22/06/2025